Simultaneous Determination of Fifteen Heterocyclic Aromatic Amines in the Urineof Smokers and Nonsmokers Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
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- 英文论文题名：Simultaneous Determination of Fifteen Heterocyclic Aromatic Amines in the Urineof Smokers and Nonsmokers Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
- 论文作者：付瑜锋 ; Ge Zhao ; Sheng Wang ; Jingjing Yu ; Fuwei Xie ; Hui Wang ; Jianping Xie
- 英文论文作者：Yufeng Fu ; Ge Zhao ; Sheng Wang ; Jingjing Yu ; Fuwei Xie ; Hui Wang ; Jianping Xie
- 年：2016
- 作者机构：河南中烟技术中心;
- 英文论文关键词：heterocyclic aromatic amines ; urine ; smoker and nonsmoker ; UHPLC-MS/MS
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
- 语种：英文
- 分类号：R446.12
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：23
- 文件大小：2040k
- 原文格式：D
摘要
A sensitive UHPLC-MS/MS method to simultaneously determine fifteen heterocyclic aromatic amines(HAAs) was developed and applied analyze of human urine. The detection limit of the fifteen HAAs was 0.80-6.06 pg/m L and the quantitation limit was 2.65-20.2pg/m L. The intra-day and inter-day precisions of all HAAs were ≤10%. Based on the high sensitivity and good precision, the method was successively applied to analyzethe urine of smokers and nonsmokers. Ten HAAs were detected, analyzed and compared between the two groups,and the analytical results showed that cigarette smoke could increase the exposures to 2-amino-9H-pyrido[2,3-b]indole(AαC) and 2-amino-1,6-dimethylimidazo[4,5-b]-pyridine(DMIP). This work is the first report that ten HAAs were simultaneously detected, and is the first comprehensive study of HAA exposure induced by cigarette smoking.
A sensitive UHPLC-MS/MS method to simultaneously determine fifteen heterocyclic aromatic amines(HAAs) was developed and applied analyze of human urine. The detection limit of the fifteen HAAs was 0.80-6.06 pg/m L and the quantitation limit was 2.65-20.2pg/m L. The intra-day and inter-day precisions of all HAAs were ≤10%. Based on the high sensitivity and good precision, the method was successively applied to analyzethe urine of smokers and nonsmokers. Ten HAAs were detected, analyzed and compared between the two groups,and the analytical results showed that cigarette smoke could increase the exposures to 2-amino-9H-pyrido[2,3-b]indole(AαC) and 2-amino-1,6-dimethylimidazo[4,5-b]-pyridine(DMIP). This work is the first report that ten HAAs were simultaneously detected, and is the first comprehensive study of HAA exposure induced by cigarette smoking.
A sensitive UHPLC-MS/MS method to simultaneously determine fifteen heterocyclic aromatic amines(HAAs) was developed and applied analyze of human urine. The detection limit of the fifteen HAAs was 0.80-6.06 pg/m L and the quantitation limit was 2.65-20.2pg/m L. The intra-day and inter-day precisions of all HAAs were ≤10%. Based on the high sensitivity and good precision, the method was successively applied to analyzethe urine of smokers and nonsmokers. Ten HAAs were detected, analyzed and compared between the two groups,and the analytical results showed that cigarette smoke could increase the exposures to 2-amino-9H-pyrido[2,3-b]indole(AαC) and 2-amino-1,6-dimethylimidazo[4,5-b]-pyridine(DMIP). This work is the first report that ten HAAs were simultaneously detected, and is the first comprehensive study of HAA exposure induced by cigarette smoking.
引文
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[37]F.Zhong,L.Huimin,X.Fuwei,X.Qiaoling,S.Wang,Tob.Sci.Technol.250(2008)39-44.
[2]H.Frederiksen,Mol.Nutr.Food Res.49(2005)263-273.
[3]T.Sugimura,K.Wakabayashi,H.Nakagama,M.Nagao,Cancer Sci.95(2004)290-299.
[4]R.J.Turesky,L.Le Marchand,Chem.Res.Toxicol.24(2011)1169-1214.
[5]M.Sanz Alaejos,A.M.Afonso,Compr.Rev.Food Sci.F.10(2011)52-108.
[6]H.Frederiksen,H.Frandsen,W.Pfau,Carcinogenesis 25(2004)1525-1533.
[7]W.Pfau,K.Skog,J.Chromatogr.B 802(2004)115-126.
[8]G.Nauwelaers,E.E.Bessette,D.Gu,Y.Tang,J.Rageul,V.Fessard,J.M.Yuan,M.C.Yu,S.Langouet,R.J.Turesky,Chem.Res.Toxicol.24(2011)913-925.
[9]J.Alexander,R.Reistada,S.Hegstada,H.Frandsenc,K.Ingebrigtsenb,J.E.Paulsena,G.Bechera,Food Chem.Toxicol.40(2002)1131-1137.
[10]E.Richling,C.Decker,D.Haring,M.Herderich,P.Schreier,J.Chromatogr.A 791(1997)71-77.
[11]J.Lezamiz,T.Barri,J.A.Jonsson,K.Skog,Anal.Bioanal.Chem.390(2008)689-696.
[12]H.Frandsen,Food Chem.Toxicol.46(2008)3200-3205.
[13]F.U.Shah,T.Barri,J.A.Jonsson,K.Skog,J.Chromatogr.B 870(2008)203-208.
[14]R.Busquets,J.A.Jonsson,H.Frandsen,L.Puignou,M.T.Galceran,K.Skog,Mol.Nutr.Food Res.53(2009)1496-1504.
[15]R.Reistad,S.Nyholm,L.Haug,G.Becher,J.Alexander,Biomarkers 4(1999)263-271.
[16]S.Hegstad,E.Lundanes,R.Reistad,L.S.Haug,G.Becher,J.Alexander,Chromatographia52(2000)499-504.
[17]F.De Andres,M.Zougagh,G.Castaneda,J.L.Sanchez-Rojas,A.Rios,Talanta 83(2011)1562-1567.
[18]F.De Andres,M.Zougagh,G.Castaneda,A.Rios,Anal.Bioanal.Chem.397(2010)223-231.
[19]H.-J.Cha,N.-H.Kim,E.-K.Jeong,Y.-C.Na,Bull.Korean Chem.Soc.31(2010)2322-2328.
[20]X.-Q.Fei,C.Li,X.-D.Yu,H.-Y.Chen,J.Chromatogr.B 854(2007)224-229.
[21]S.Sentellas,E.Moyano,L.Puignou,M.T.Galceran,J.Chromatogr.A 1032(2004)193-201.
[22]R.Reistad,O.J.Rossland,K.J.Latva-Kala,T.Rasmussen,R.Vikse,G.Becher,J.Alexander,Food Chem.Toxicol.35(1997)945-955.
[23]L.C.R.Kidd,W.G.Stillwell,M.C.Yu,J.S.Wishnok,P.L.Skipper,R.K.Ross,B.E.Henderson,S.R.Tannenbaum,Cancer Epidem.Biomar.8(1999)439-445.
[24]R.D.Holland,J.Taylor,L.Schoenbachler,R.C.Jones,J.P.Freeman,D.W.Miller,B.G.Lake,N.J.Gooderham,R.J.Turesky,Chem.Res.Toxicol.17(2004)1121-1136.
[25]R.J.Turesky,J.M.Yuan,R.Wang,S.Peterson,M.C.Yu,Cancer Epidem.Biomar.16(2007)1554-1560.
[26]W.Haiyan,G.Zhao,X.Fuwei,S.Wang,Tob.Sci.Technol.271(2010)28-34.
[27]L.Q.Zhang,D.L.Ashley,C.H.Watson,Nicotine.Tob.Res.13(2011)120-126.
[28]S.Liu,L.T.Taylor,M.F.Borgerding,W.M.Coleman,III,B.R.Bombick,Beitraege zur Tabakforschung International 25(2013)550-562.
[29]M.Peluso,M.Castegnaro,C.Malaveille,M.Friesen,L.Garren,A.Hautefeuille,P.Vineis,F.Kadlubar,H.Bartsch,Carcinogenesis 12(1991)713-717.
[30]D.Gu,M.M.Raymundo,F.F.Kadlubar,R.J.Turesky,Anal.Chem.83(2011)1093-1101.
[31]L.Cardenes,J.H.Ayala,A.M.Afonso,V.Gonzalez,J.Chromatogr.A 1030(2004)87-93.
[32]J.Bang,H.Frandsen,K.Skog,Chromatographia 60(2004)651-655.
[33]S.H.Sun,J.P.Xie,F.W.Xie,Y.L.Zong,J.Chromatogr.A 1179(2008)89-95.
[34]S.Saha,R.Mistri,B.C.Ray,J.Chromatogr.A 1216(2009)3059-3063.
[35]H.Ushiyama,A.Oguri,Y.Totsuka,H.Itoh,T.Sugimura,K.Wakabayashi,P.Jpn.Acad.B-Phys.71(1995)57-60.
[36]R.Nishigaki,Y.Totsuka,H.Kataoka,H.Ushiyama,S.Goto,T.Akasu,T.Watanabe,T.Sugimura,K.Wakabayashi,Cancer Epidem.Biomar.16(2007)151-156.
[37]F.Zhong,L.Huimin,X.Fuwei,X.Qiaoling,S.Wang,Tob.Sci.Technol.250(2008)39-44.