Mating type gene organization in the mushroom forming fungus Flammulinavelutipes
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- 英文论文题名：Mating type gene organization in the mushroom forming fungus Flammulinavelutipes
- 论文作者：Wei Wang ; Lingdan Lian ; Ping Xu ; Tiansheng Chou ; Irum Mukhtar ; Aron Osakina ; Muhammad Waqas ; Bingzhi Chen ; Xinrui Liu ; Fang Liu ; Baogui Xie ; Arend F.van Peer
- 英文论文作者：Wei Wang ; Lingdan Lian ; Ping Xu ; Tiansheng Chou ; Irum Mukhtar ; Aron Osakina ; Muhammad Waqas ; Bingzhi Chen ; Xinrui Liu ; Fang Liu ; Baogui Xie ; Arend F.van Peer ; Mycological Research Center ; College of Life Sciences ; Fujian Agriculture and Forestry University ; College of Crop Sciences ; Fujian Agriculture and Forestry University ; College of Food Sciences ; Fujian Agriculture and Forestry University
- 年：2016
- 作者机构：Mycological Research Center,College of Life Sciences,Fujian Agriculture and Forestry University;College of Crop Sciences,Fujian Agriculture and Forestry University;College of Food Sciences,Fujian Agriculture and Forestry University;
- 会议召开时间：2016-08-19
- 会议录名称：中国菌物学会2016年学术年会论文摘要集
- 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
- 语种：英文
- 分类号：S646
- 学会代码：ZGVL
- 会议名称：中国菌物学会2016年学术年会
- 会议地点：中国福建福州
- 主办单位：中国菌物学会
- 学会名称：中国菌物学会
- 页数：1
- 文件大小：717k
- 原文格式：D
- 会议级别：全国
摘要
Mating involvesthe transition from a self-sterileinto a fertile mycelium.As a prerequisite for the production of fruiting bodies,this is a fundamental step in the life cycle of mushroom forming fungi.During mating,two genetically distinct monokaryons called mating types,will fuse and establish a dikaryon.The compatibilityof mating types as well as the development of a dikaryonare controlled by independent gene clusters atthe MAT-A and the MAT-B loci.These MAT loci have been studied poorly for the majority of mushroom forming fungi.To deepen our understanding of mating,we identified and mapped the structures of a series of MAT loci in the important edible mushroom Flammulinavelutipes.The results showed that the MAT-A and the MAT-B loci of F.velutipes each contained two separate,functionally redundantsubloci(Aa/Ab and Ba/Bb).Depending on the interacting mating types,different MAT-A and MAT-Bsublociare predicted to control the mating process.Segregation analysis demonstrated that new combinations of MAT-Aa and MAT-Absubloci could be generated at a low frequency during meiosis.Similarly,we demonstrated different combinations of MAT-Ba and MATBbsubloci.This means that F.velutipes can generate new mating types not only by recombining MAT-A with MAT-B loci(i.e.a typical tetrapolar mating type system),but also can occasionally create new MAT-A and MAT-B mating types.The resultsof our study provide important directions for further research on mating.Mating type genes of loci that were indicated to control mating in specific combinations are currently subjected to transformation assays that will confirm(or reject) their control over their respective A or B pathway.The resulting mutants will be used to explore thedownstreamgenetic mechanisms of mating in F.velutipes.
Mating involvesthe transition from a self-sterileinto a fertile mycelium.As a prerequisite for the production of fruiting bodies,this is a fundamental step in the life cycle of mushroom forming fungi.During mating,two genetically distinct monokaryons called mating types,will fuse and establish a dikaryon.The compatibilityof mating types as well as the development of a dikaryonare controlled by independent gene clusters atthe MAT-A and the MAT-B loci.These MAT loci have been studied poorly for the majority of mushroom forming fungi.To deepen our understanding of mating,we identified and mapped the structures of a series of MAT loci in the important edible mushroom Flammulinavelutipes.The results showed that the MAT-A and the MAT-B loci of F.velutipes each contained two separate,functionally redundantsubloci(Aa/Ab and Ba/Bb).Depending on the interacting mating types,different MAT-A and MAT-Bsublociare predicted to control the mating process.Segregation analysis demonstrated that new combinations of MAT-Aa and MAT-Absubloci could be generated at a low frequency during meiosis.Similarly,we demonstrated different combinations of MAT-Ba and MATBbsubloci.This means that F.velutipes can generate new mating types not only by recombining MAT-A with MAT-B loci(i.e.a typical tetrapolar mating type system),but also can occasionally create new MAT-A and MAT-B mating types.The resultsof our study provide important directions for further research on mating.Mating type genes of loci that were indicated to control mating in specific combinations are currently subjected to transformation assays that will confirm(or reject) their control over their respective A or B pathway.The resulting mutants will be used to explore thedownstreamgenetic mechanisms of mating in F.velutipes.
Mating involvesthe transition from a self-sterileinto a fertile mycelium.As a prerequisite for the production of fruiting bodies,this is a fundamental step in the life cycle of mushroom forming fungi.During mating,two genetically distinct monokaryons called mating types,will fuse and establish a dikaryon.The compatibilityof mating types as well as the development of a dikaryonare controlled by independent gene clusters atthe MAT-A and the MAT-B loci.These MAT loci have been studied poorly for the majority of mushroom forming fungi.To deepen our understanding of mating,we identified and mapped the structures of a series of MAT loci in the important edible mushroom Flammulinavelutipes.The results showed that the MAT-A and the MAT-B loci of F.velutipes each contained two separate,functionally redundantsubloci(Aa/Ab and Ba/Bb).Depending on the interacting mating types,different MAT-A and MAT-Bsublociare predicted to control the mating process.Segregation analysis demonstrated that new combinations of MAT-Aa and MAT-Absubloci could be generated at a low frequency during meiosis.Similarly,we demonstrated different combinations of MAT-Ba and MATBbsubloci.This means that F.velutipes can generate new mating types not only by recombining MAT-A with MAT-B loci(i.e.a typical tetrapolar mating type system),but also can occasionally create new MAT-A and MAT-B mating types.The resultsof our study provide important directions for further research on mating.Mating type genes of loci that were indicated to control mating in specific combinations are currently subjected to transformation assays that will confirm(or reject) their control over their respective A or B pathway.The resulting mutants will be used to explore thedownstreamgenetic mechanisms of mating in F.velutipes.
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