小刺猴头多糖对大鼠慢性萎缩性胃炎的实验研究
摘要
小刺猴头多糖(Hericium Caput medusae Polysaccharide,HP)是以小刺猴头子实体包括菌丝体为原料采用现代方法提取而成。小刺猴头别名针猴头菌,是猴头菌科真菌小刺猴头的子实体和发酵菌丝体。民间以子实体入药,有利肠胃、助消化的作用,可用于治疗慢性萎缩性胃炎、浅表性胃炎、消化性胃溃疡和十二指肠球部溃疡及其它原因引起的胃痛、胃酸过多等疾病。为探讨小刺猴头多糖对慢性萎缩性胃炎的治疗作用及其机制,本文进行了如下几方面的研究:
1.采用2%水杨酸钠和30%酒精混合溶液灌胃,并结合劳累、饥饱失常等多因素方法复制大鼠慢性萎缩性胃炎模型,观察小刺猴头多糖对慢性萎缩性胃炎的影响。结果表明:小刺猴头多糖能使胃液中游离酸度和蛋白酶活性增强,血清胃泌素含量降低,血浆中cAMP含量升高而cGMP含量有所降低。病理检查结果表明,小刺猴头多糖能明显抑制胃窦及胃体粘膜萎缩变薄。
2.采用碳末法进行小鼠小肠推进运动实验,观察小刺猴头多糖对小鼠胃肠推进功能的影响。结果表明小刺猴头多糖能明显增强小鼠胃肠的推进功能。
3.采用耳肿胀法和扭体法观察小刺猴头多糖的抗炎镇痛作用。结果表明:小刺猴头多糖能明显的抑制二甲苯引起的急性炎症及冰醋酸导致的扭体反应,有良好的抗炎镇痛作用。
实验结果表明:小刺猴头多糖能够改善慢性萎缩性胃炎的胃内环境;降低过高的血清胃泌素含量,升高血浆中的cAMP的含量,对胃粘膜有很好的保护作用;并且小刺猴头多糖能明显增强胃肠推进功能,有明显的抗炎镇痛作用。以上可能是小刺猴头多糖治疗慢性萎缩性胃炎,缓解其临床症状的理论依据。
Chronic atrophic gastritis (CAG) is a common disease of digestive system, which demonstrates pathological changes in focal and global gastric mucosa, with shrinkage and functional decrease in different level for gastric secrete. At present, CAG, especially with intestinal metaplasia (IM) and/or atypical hyperplasia, is considered to be precancerous lesions of gastric cancer (PLGC). Researchers have been focusing on CAG to pursuit for corresponding pathogenesis and pathological mechanism, which is not clearly defined up to today. CAG, a high occurrence and canceration disease, is considered as a kind of disorder resulted from multiple factors. No therapeutical strategy has been successfully developed with modern medical protocals. However, in traditional Chinese medicine fields, many exciting practices have been conducted in treating spleen and stomach disorders, which indicate a meaningful potential for CAG therapy.
Hericium caput medusae is a kind of precious bacterium, which can eat and be used as medicine. In private, Hericium caput medusae’s fruiting body is always used as a drug to treat CAG, superficial gastritis (SG), gastric ulcer (GU), duodenal ulcer (DU) and other gastropathies. The main components of the extracts from fruiting body after fermentation are polysaccharide, monosaccharide, protein, amino acids and so on. It has been proved that Hericium caput medusae is of great value for
1.采用2%水杨酸钠和30%酒精混合溶液灌胃,并结合劳累、饥饱失常等多因素方法复制大鼠慢性萎缩性胃炎模型,观察小刺猴头多糖对慢性萎缩性胃炎的影响。结果表明:小刺猴头多糖能使胃液中游离酸度和蛋白酶活性增强,血清胃泌素含量降低,血浆中cAMP含量升高而cGMP含量有所降低。病理检查结果表明,小刺猴头多糖能明显抑制胃窦及胃体粘膜萎缩变薄。
2.采用碳末法进行小鼠小肠推进运动实验,观察小刺猴头多糖对小鼠胃肠推进功能的影响。结果表明小刺猴头多糖能明显增强小鼠胃肠的推进功能。
3.采用耳肿胀法和扭体法观察小刺猴头多糖的抗炎镇痛作用。结果表明:小刺猴头多糖能明显的抑制二甲苯引起的急性炎症及冰醋酸导致的扭体反应,有良好的抗炎镇痛作用。
实验结果表明:小刺猴头多糖能够改善慢性萎缩性胃炎的胃内环境;降低过高的血清胃泌素含量,升高血浆中的cAMP的含量,对胃粘膜有很好的保护作用;并且小刺猴头多糖能明显增强胃肠推进功能,有明显的抗炎镇痛作用。以上可能是小刺猴头多糖治疗慢性萎缩性胃炎,缓解其临床症状的理论依据。
Chronic atrophic gastritis (CAG) is a common disease of digestive system, which demonstrates pathological changes in focal and global gastric mucosa, with shrinkage and functional decrease in different level for gastric secrete. At present, CAG, especially with intestinal metaplasia (IM) and/or atypical hyperplasia, is considered to be precancerous lesions of gastric cancer (PLGC). Researchers have been focusing on CAG to pursuit for corresponding pathogenesis and pathological mechanism, which is not clearly defined up to today. CAG, a high occurrence and canceration disease, is considered as a kind of disorder resulted from multiple factors. No therapeutical strategy has been successfully developed with modern medical protocals. However, in traditional Chinese medicine fields, many exciting practices have been conducted in treating spleen and stomach disorders, which indicate a meaningful potential for CAG therapy.
Hericium caput medusae is a kind of precious bacterium, which can eat and be used as medicine. In private, Hericium caput medusae’s fruiting body is always used as a drug to treat CAG, superficial gastritis (SG), gastric ulcer (GU), duodenal ulcer (DU) and other gastropathies. The main components of the extracts from fruiting body after fermentation are polysaccharide, monosaccharide, protein, amino acids and so on. It has been proved that Hericium caput medusae is of great value for
引文
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[2] 张禹.慢性胃病的三联疗法.人民军医,2000,43(3):156
[3] 陈朝元,王岩.慢性萎缩性胃炎的中医药研究进展.福建中医学院学报,2002, 12(2):59-61
[4] 乔樵.慢性萎缩性胃炎癌前病变的中医证治[J].浙江中医学院学报,1997,21 (6):7
[5] 陈天锡.运用活血化瘀法治疗慢性萎缩性胃炎 61 例[J].浙江中医杂志, 1992, 27(3):49
[6] 鲁家法,马琼英,崔世镐,等.中医辨证治疗萎缩性胃炎 40 例[J].湖北中医杂志, 1997,19(4):29
[7] 侯树德.慢性萎缩性胃炎辨治体会[J]现代中西医结合杂志,2000,9(13):1262
[8] 林小武.中医药治疗慢性萎缩性胃炎临床研究进展[J].北京中医,1999,1:56- 57
[9] 李乾构.萎缩性胃炎辨证的证型探讨[J].北京中医,1992,3:9-10
[10] 陈瑜,吴丹明,兰春英.辨证治疗慢性胃炎癌前病变 45 例疗效分析[J].中医杂志,1994,35(7):418
[11] 杨炳奎,沈嘉美.中药治疗胃癌前期病变的临床观察[J].中西医结合脾胃杂志,1997,5(2):73
[12] 闫肃,李志琴.一贯煎治疗萎缩性胃炎 118 例[J].陕西中医,1999,20(4):161- 163
[13] 郝淑然,李培英,孙寒光.胃丹胶囊治疗慢性萎缩性胃炎临床观察[J].中国医药学报,1997,12(3):35
[14] 郭喜军.摩罗丹治疗萎缩性胃炎肠上皮化生 200 例疗效观察[J].北京中医药大学学报,1994,17(1):33
[15] Wantanabl H. Experimentally induced intestinal eletaplasha in wistar pasts by X-ray: radiation. Gastroenterology, 1978, 15:793
[16] 姒健敏,吴加国.鼠慢性萎缩性胃炎模型的建立及致萎缩因素探讨.中华消化杂志,2001,2(21):75
[17] 李秋娟,仲来福,叶建新,等.大鼠实验性慢性萎缩性胃炎与胃黏膜生长抑素、胃泌素关系的研究[J].大连医科大学学报,1999,21(2):89-91
[18] 张旭晨.消痞灵冲剂对胃癌前期病变大鼠胃粘膜 AgNOR 影响的定量研究.中国肿瘤,1994,3(4):32
[19] 陆为民.大鼠慢性萎缩性胃炎癌前病变气虚血瘀证动物模型的研制.南京中医药大学学报,2000,16(3):156
[20] 李兆申.综合法制作大鼠萎缩性胃炎模型的实验研究.中华医学杂志,1992, 72(2):81
[21] 张淑芹,赵林山,郑继奎.慢性萎缩性胃炎动物模型的复制[J].哈尔滨师范大学自然科学学报,2001,17(6):81-83
[22] Baskerville A, Newell DG. Naturally demurring chronic gastritis is C pylori infection in the monkey: A Potential Model for Gastritis in man. Gut, 1988, 29:465
[23] Fox JG, Correa P, Taylor NS, et al. Helicobacter mustelae-associated gastritis in ferrets: An animal model of helicobacter pylori gastritis in human. Gastroenterology, 1990, 99:352
[24] 李弼民.BALB/C 小鼠 Hp 感染胃炎动物模型的建立和免疫治疗实验研究.现代诊断与治疗,2001,12(3):131
[25] 张斌,高金亮.健脾化瘀对脾虚慢性萎缩性胃炎大鼠胃肠功能的影响[J].中国中西医结合脾胃杂志,1995,3(1):41-43
[26] 陈小野,邹世洁.大鼠 CAG 证病结合模型胃粘膜病理研究.实验动物科学与管理,2001,18(3):5
[27] 危北海,张占海,杨丽彩,等.胃安素治疗慢性萎缩性胃炎的临床与实验研究[J].华人消化杂志,1998,6(2):114-117
[28] 罗巅辉,王昭晶,梁忠岩.小刺猴头菌子实体多糖的分离、纯化及初步研究.福建中医学院学报,2005,15(4):32
[29] 李悦,陈岩,张新建.复方猴头冲剂治疗慢性胃炎 114 例疗效分析[J].山东医药,1994,34(9):62
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