早期梅毒血清固定患者治疗前后外周血淋巴细胞及血清IL-2、IL-10检测
摘要
目的研究早期梅毒血清固定患者外周血淋巴细胞亚群及血清IL-2、IL-10水平的变化,探讨苄星青霉素G联合乌体林斯治疗对早期梅毒血清固定患者外周血淋巴细胞亚群、血清IL-2、IL-10水平的影响。
方法1.采用酶联免疫吸附试验(ELISA)检测正常对照组及早期梅毒血清固定患者血清IL-2、IL-10水平。2.采用甲苯胺红不加热血清试验(TRUST)检测经苄星青霉素G联合乌体林斯治疗前后非梅毒螺旋体抗体滴度的变化。3.早期梅毒血清固定患者经苄星青霉素G联合乌体林斯治疗前后,分别采用流式细胞仪(FCM)、ELISA法检测外周血淋巴细胞亚群、血清IL-2及IL-10水平。
结果1.治疗前4例患者TRUST滴度为1:2,15例患者TRUST滴度为1:4,1例患者TRUST滴度为1:8;治疗后3个月9例患者TRUST滴度转阴,3例患者TRUST滴度为1:1,4例患者TRUST滴度为1:2,4例患者TRUST滴度为1:4;治疗后6个月10例患者TRUST滴度转阴,5例患者TRUST滴度为1:1,3例患TRUST滴度为1:2;2例患者TRUST滴度为1:4。2.早期梅毒血清固定患者血清IL-2、IL-10水平变化:早期梅毒血清固定患者血清IL-2水平显著低于正常对照组(p=0.018);IL-10水平显著高于正常对照组(p=0.03)。3.经苄星青霉素G联合乌体林斯治疗后外周血淋巴细胞亚群变化:治疗后3个月CD3+T淋巴细胞显著高于治疗前
Objective: To study the changes of peripheral blood lymphocyte subsets and levels of serum Interleukin-2 and Interleukin-10 in early syphilis serofast patients and to investigate the effects of Benzathine penicillin G and wutilinsi on peripheral blood lymphocyte subsets and levels of serum Interleukin-2 and Interleukin-10.
Methods:①. The levels of serum IL-2 and IL-10 were detected by ELISA in early syphilis serofast patients and in normal persons.②. The level of antibody titre was detected by tolulized red unheated serum test (TRUST) before and after treatment.③. Peripheral blood lymphocyte subsets were assessed with flow cytometry (FCM) and the levels of serum IL-2 and IL-10 were detected by ELISA before and after treatment.
Results:①.Before treatment, the TRUST titre was 1:2 in 4 patients, 1:4 in 15 patients, 1:8 in 1 patient; after treatment 3 months later, the TRUST titre turned negative in 9 patients, 1:1 in 3 patients, 1:2 in 4 patients, 1:4 in 4 patients; after treatment 6 months later, the TRUST titre turned negative in 10 patients,1:1 in 5 patients, 1:2 in 3 patients, 1:4 in 2
方法1.采用酶联免疫吸附试验(ELISA)检测正常对照组及早期梅毒血清固定患者血清IL-2、IL-10水平。2.采用甲苯胺红不加热血清试验(TRUST)检测经苄星青霉素G联合乌体林斯治疗前后非梅毒螺旋体抗体滴度的变化。3.早期梅毒血清固定患者经苄星青霉素G联合乌体林斯治疗前后,分别采用流式细胞仪(FCM)、ELISA法检测外周血淋巴细胞亚群、血清IL-2及IL-10水平。
结果1.治疗前4例患者TRUST滴度为1:2,15例患者TRUST滴度为1:4,1例患者TRUST滴度为1:8;治疗后3个月9例患者TRUST滴度转阴,3例患者TRUST滴度为1:1,4例患者TRUST滴度为1:2,4例患者TRUST滴度为1:4;治疗后6个月10例患者TRUST滴度转阴,5例患者TRUST滴度为1:1,3例患TRUST滴度为1:2;2例患者TRUST滴度为1:4。2.早期梅毒血清固定患者血清IL-2、IL-10水平变化:早期梅毒血清固定患者血清IL-2水平显著低于正常对照组(p=0.018);IL-10水平显著高于正常对照组(p=0.03)。3.经苄星青霉素G联合乌体林斯治疗后外周血淋巴细胞亚群变化:治疗后3个月CD3+T淋巴细胞显著高于治疗前
Objective: To study the changes of peripheral blood lymphocyte subsets and levels of serum Interleukin-2 and Interleukin-10 in early syphilis serofast patients and to investigate the effects of Benzathine penicillin G and wutilinsi on peripheral blood lymphocyte subsets and levels of serum Interleukin-2 and Interleukin-10.
Methods:①. The levels of serum IL-2 and IL-10 were detected by ELISA in early syphilis serofast patients and in normal persons.②. The level of antibody titre was detected by tolulized red unheated serum test (TRUST) before and after treatment.③. Peripheral blood lymphocyte subsets were assessed with flow cytometry (FCM) and the levels of serum IL-2 and IL-10 were detected by ELISA before and after treatment.
Results:①.Before treatment, the TRUST titre was 1:2 in 4 patients, 1:4 in 15 patients, 1:8 in 1 patient; after treatment 3 months later, the TRUST titre turned negative in 9 patients, 1:1 in 3 patients, 1:2 in 4 patients, 1:4 in 4 patients; after treatment 6 months later, the TRUST titre turned negative in 10 patients,1:1 in 5 patients, 1:2 in 3 patients, 1:4 in 2
引文
[1] Fraser CM, Norris SJ, Weinstock GM, et al. Complete genome sequence of treponema pallidum, the syphilis spirochete[J]. Science, 1998,281(17):375～388
[2] Wicher V, Zhao J, Dilwith R, et al. Immune abnormalities in guinea pigs with asymptomatic congenital syphilis[J]. J Pediatr Res, 1997,42(6):794～798
[3] Pope V, Sandro AL, Larsen SA, et al. Flow cytometry of peripheral lymphocyte immunophenotypes in persons infected with treponema pallidum[J]. Clin Diagn Lab Immunol, 1994,1(1):121～124
[4] 李碧清,阮光萍.梅毒病人外周血T细胞亚群特点的探讨[J].免疫学杂志, 1999,15(4):264～265
[5] 朱慧兰,曾志荣,叶兴东,等.梅毒患者细胞免疫的研究[J].中国性病艾滋病防治,2002,8(4):139
[6] 李秋涛,樊曼云,张秋堂,等.梅毒患者细胞免疫功能的检测[J].中华皮肤科杂志,2002,5(3):183～184
[7] Van Voorhis WC, Barrett LK, Koelle DM, et al. Primary and secondary syphilis lesions contain mRNA for Th1 cytokine[J]. J Infect Dis, 1996,173(2):491～495
[8] 周玉平,熊思东,阎春林,等.梅毒患者皮损中Th1/Th2细胞因子表达 与RPR试验阴转的关系[J].中华皮肤科杂志,2004,37(12):712～714
[9] Podwinska J, Eaba R, Chomiknl et al. The ability of peripheral blood mononuclear cells (PBMC) of syphilitic patients to produce IL-2[J]. FEMS Immunol Med Microbiol, 1995,12(1):12～27
[10] 曹双林,傅玲玲,符梅,等.二期梅毒患者血清中IL-6、IL-8及肿瘤坏死因子-α测定[J].中华皮肤科杂志,2001,34(6):423～424
[11] 谢文,宋继权,周自刚.一期和二期梅毒患者血清IL-2、IL-4含量的测定及意义[J].上海医学检验杂志,2003,18(3):192
[12] 黄小雄,邹循辉,钟金城.二期梅毒患者血清IL-10和12水平的检测[J].中华皮肤科杂志,2005,38(2):111
[13] 周晓明,周礼义,陈兴平.梅毒患者单一核细胞体外培养观察白介素-4,γ-干扰素的变化[J].中华皮肤科杂志,2002,35(3):185～186
[14] Podwinska J, Lusiak M, Eaba R, et, al. The pattern and level of cytokines secreted by Th1 and Th2 lymphocytes of syphilitic patients correlate to the progression of the disease[J]. FEMS Immunol Med Microbiol, 2000,28(1):1～4
[15] 陈星,翁瑞全,李惠.梅毒血清固定型研究进展[J].医学综述,2006,12(7):432～433
[16] Shetsiruli,-L-T, Gogebashvili,-N-V, Toniia,-K-S, et al, immunologic aspects of the pathogenesis of latent and sero-resistant forms of syphilis[J]. Vestn-Dermatol- Venero, 1989,(5):55～58
[17] K.A. Yuldashev, K.M. Abzoirov, some immunological indicators in sero-resistant syphilis[J]. J Eur Acad Dermatol Venerol, 2004,18(s2):486
[18] 杨日东,蔡川川,田广南,等.血清固定梅毒患者细胞免疫研究[J].中国实验诊断学,2004,8(5):477～479
[19] 杨文林,杨健,黄新宇,等.梅毒血清固定病人的脑脊液及免疫功能检测[J].中国医师杂志,2005,7(5):644～646
[20] 龚向东,姜文华,王全佩,等.我国 1979～1989 年梅毒流行病学分析[J].中国公共卫生杂志,2000,16(11):1020～1022
[21] 马聪,王芳,徐武夷.梅毒螺旋体实验室检测技术的研究进展[J].中华医院感染学杂志,2002,12(12):958
[22] 金伯泉.细胞与分子免疫学[M].第二版.北京:科学出版社,2001.501
[23] 朱迅.免疫学新进展[M].北京:人民卫生出版社,2002.277～280
[24] 田志刚.NK细胞新理论对重大疾病的新解释[J].中国免疫学杂志,2002,18(2):75～78
[25] Monsmann TR, Sad S. The expanding universe of T cell subsets: Th1, Th2 and more[J]. Immunol Today, 1996,17(3):138～146
[26] Muraille E, Leo O. Revisiting the Th1/Th2 paradigm[J]. Scand J Immunol, 1998,47:1～9
[1] 李志文,现代性传播疾病[M].北京:人民卫生出版社,1991,174
[2] 施 辛,戚宝康.头孢曲松治疗早期梅毒血清固定近期疗效观察[J].中国麻风皮肤病杂志,2002,18(4):348-349
[3] 候 青,孔.立梅毒患者12例治疗后血清非梅毒螺旋体抗原试验长期未转阴的临床分析[J].职业与健康,2003,19(5):126-127
[4] Lukehart SA, Macrolide resistance in treponema pallidum in the United States and Ireland[J]. N Engl J Med, 2004, 351(2):154-8
[5] 杨立刚,张锡宝,钟 山,等.阿齐霉素对早期梅毒的治疗效果(附14例疗效观察)[J].中国热带医学,2004,4(5):771-772
[6] Fiumara NJ. Serologic responses to treatment of 128 patients with late latent syphilis[J]. Sex Transm Dis, 1979,6(4):243-246
[7] Fiumara NJ. treatment of primary and secondary syphilis: serologic response[J]. J Am Acad Dermatol, 1986,14(3):487-91
[8] Kastner,-R-J; Malone,-J-L; Decker,-C-F, Syphilitic osteitis in a patient with secondary syphilis and concurrent human immunodeficiency virus infection[J]. Clin-Infect-Dis, 1994,18(2):250-252
[9] Dowell,-M-E, Ross,-P-G, Musher,-D-M,Cate,et al.Response of latent syphilis or neurosyphilis to ceftriaxone therapy in persons infected with human immunodeficiency virus[J]. Am-J-Med, 1992,93(5):481-8
[10] 杨日东,李 季,梁艳华,等.RPR 持续阳性梅毒患者脑脊液梅毒检测的意义[J].中国皮肤性病学杂志,2004,18(3):155-156
[11] Romanowski B ,Sutherland R, Fick GH, et al. Serologic response to treatment of infectious syphilis[J]. Ann Intern Med, 1991,114:1005-1009
[12] 尹建奇,李晓娥,刘平英,等.2592 份血清中梅毒血清学生物学假阳性结果的临床分析[J].中国皮肤性病学杂志,2001,15(5):317-318
[13] Funnyé AS;Akhtar AJ. Syphilis and human immunodeficiency virus co-infection[J]. J Natl Med Assoc, 2003,95(5):363-382
[14] Smith NH, Musher DM, Huang DB, et al. response of HIV-infected patients with asymptomatic syphilis to intensive intramuscular therapy with ceftriaxone or procaine penicillin[J].Int J STD AIDS, 2004,15(5),328-332
[15] Shetsiruli,-L-T, Gogebashvili,-N-V, Toniia,-K-S, et al,immunologic aspects of the pathogenesis of latent and seroresistant forms of syphilis[J].Vestn-Dermatol-Venero, 1989,(5):55-58
[16] K.A. Yuldashev, K.M. Abzoirov, some immunological indicators in seroresistant syphilis[J].J Eur Acad Dermatol Venerol,2004,18(s2):486
[17] 杨日东,蔡川川,田广南,等.血清固定梅毒患者细胞免疫研究[J].中国实验诊断学,2004,8(5):477-479
[18] Kaminski,-A, Gede,-K, Owsinska,-H, et al. results of the treatment of neurosyphilis and seroresistant latent syphilis by intravenous administration of potassium salt of penicillin G[J]. Przegl-Dermatol,1989,76(3):256-261
[19] 昊建诚,林品东,陈天明,等。碘化钾治疗梅毒血清固定疗效观察[J].河北医学,2004,10(10):894-895
[20] 程喜平,雷公藤、强的松对 RPR、TPPA 滴度变化的影响[J].岭南皮肤性病科杂志,2002,9(4):241-244
[2] Wicher V, Zhao J, Dilwith R, et al. Immune abnormalities in guinea pigs with asymptomatic congenital syphilis[J]. J Pediatr Res, 1997,42(6):794～798
[3] Pope V, Sandro AL, Larsen SA, et al. Flow cytometry of peripheral lymphocyte immunophenotypes in persons infected with treponema pallidum[J]. Clin Diagn Lab Immunol, 1994,1(1):121～124
[4] 李碧清,阮光萍.梅毒病人外周血T细胞亚群特点的探讨[J].免疫学杂志, 1999,15(4):264～265
[5] 朱慧兰,曾志荣,叶兴东,等.梅毒患者细胞免疫的研究[J].中国性病艾滋病防治,2002,8(4):139
[6] 李秋涛,樊曼云,张秋堂,等.梅毒患者细胞免疫功能的检测[J].中华皮肤科杂志,2002,5(3):183～184
[7] Van Voorhis WC, Barrett LK, Koelle DM, et al. Primary and secondary syphilis lesions contain mRNA for Th1 cytokine[J]. J Infect Dis, 1996,173(2):491～495
[8] 周玉平,熊思东,阎春林,等.梅毒患者皮损中Th1/Th2细胞因子表达 与RPR试验阴转的关系[J].中华皮肤科杂志,2004,37(12):712～714
[9] Podwinska J, Eaba R, Chomiknl et al. The ability of peripheral blood mononuclear cells (PBMC) of syphilitic patients to produce IL-2[J]. FEMS Immunol Med Microbiol, 1995,12(1):12～27
[10] 曹双林,傅玲玲,符梅,等.二期梅毒患者血清中IL-6、IL-8及肿瘤坏死因子-α测定[J].中华皮肤科杂志,2001,34(6):423～424
[11] 谢文,宋继权,周自刚.一期和二期梅毒患者血清IL-2、IL-4含量的测定及意义[J].上海医学检验杂志,2003,18(3):192
[12] 黄小雄,邹循辉,钟金城.二期梅毒患者血清IL-10和12水平的检测[J].中华皮肤科杂志,2005,38(2):111
[13] 周晓明,周礼义,陈兴平.梅毒患者单一核细胞体外培养观察白介素-4,γ-干扰素的变化[J].中华皮肤科杂志,2002,35(3):185～186
[14] Podwinska J, Lusiak M, Eaba R, et, al. The pattern and level of cytokines secreted by Th1 and Th2 lymphocytes of syphilitic patients correlate to the progression of the disease[J]. FEMS Immunol Med Microbiol, 2000,28(1):1～4
[15] 陈星,翁瑞全,李惠.梅毒血清固定型研究进展[J].医学综述,2006,12(7):432～433
[16] Shetsiruli,-L-T, Gogebashvili,-N-V, Toniia,-K-S, et al, immunologic aspects of the pathogenesis of latent and sero-resistant forms of syphilis[J]. Vestn-Dermatol- Venero, 1989,(5):55～58
[17] K.A. Yuldashev, K.M. Abzoirov, some immunological indicators in sero-resistant syphilis[J]. J Eur Acad Dermatol Venerol, 2004,18(s2):486
[18] 杨日东,蔡川川,田广南,等.血清固定梅毒患者细胞免疫研究[J].中国实验诊断学,2004,8(5):477～479
[19] 杨文林,杨健,黄新宇,等.梅毒血清固定病人的脑脊液及免疫功能检测[J].中国医师杂志,2005,7(5):644～646
[20] 龚向东,姜文华,王全佩,等.我国 1979～1989 年梅毒流行病学分析[J].中国公共卫生杂志,2000,16(11):1020～1022
[21] 马聪,王芳,徐武夷.梅毒螺旋体实验室检测技术的研究进展[J].中华医院感染学杂志,2002,12(12):958
[22] 金伯泉.细胞与分子免疫学[M].第二版.北京:科学出版社,2001.501
[23] 朱迅.免疫学新进展[M].北京:人民卫生出版社,2002.277～280
[24] 田志刚.NK细胞新理论对重大疾病的新解释[J].中国免疫学杂志,2002,18(2):75～78
[25] Monsmann TR, Sad S. The expanding universe of T cell subsets: Th1, Th2 and more[J]. Immunol Today, 1996,17(3):138～146
[26] Muraille E, Leo O. Revisiting the Th1/Th2 paradigm[J]. Scand J Immunol, 1998,47:1～9
[1] 李志文,现代性传播疾病[M].北京:人民卫生出版社,1991,174
[2] 施 辛,戚宝康.头孢曲松治疗早期梅毒血清固定近期疗效观察[J].中国麻风皮肤病杂志,2002,18(4):348-349
[3] 候 青,孔.立梅毒患者12例治疗后血清非梅毒螺旋体抗原试验长期未转阴的临床分析[J].职业与健康,2003,19(5):126-127
[4] Lukehart SA, Macrolide resistance in treponema pallidum in the United States and Ireland[J]. N Engl J Med, 2004, 351(2):154-8
[5] 杨立刚,张锡宝,钟 山,等.阿齐霉素对早期梅毒的治疗效果(附14例疗效观察)[J].中国热带医学,2004,4(5):771-772
[6] Fiumara NJ. Serologic responses to treatment of 128 patients with late latent syphilis[J]. Sex Transm Dis, 1979,6(4):243-246
[7] Fiumara NJ. treatment of primary and secondary syphilis: serologic response[J]. J Am Acad Dermatol, 1986,14(3):487-91
[8] Kastner,-R-J; Malone,-J-L; Decker,-C-F, Syphilitic osteitis in a patient with secondary syphilis and concurrent human immunodeficiency virus infection[J]. Clin-Infect-Dis, 1994,18(2):250-252
[9] Dowell,-M-E, Ross,-P-G, Musher,-D-M,Cate,et al.Response of latent syphilis or neurosyphilis to ceftriaxone therapy in persons infected with human immunodeficiency virus[J]. Am-J-Med, 1992,93(5):481-8
[10] 杨日东,李 季,梁艳华,等.RPR 持续阳性梅毒患者脑脊液梅毒检测的意义[J].中国皮肤性病学杂志,2004,18(3):155-156
[11] Romanowski B ,Sutherland R, Fick GH, et al. Serologic response to treatment of infectious syphilis[J]. Ann Intern Med, 1991,114:1005-1009
[12] 尹建奇,李晓娥,刘平英,等.2592 份血清中梅毒血清学生物学假阳性结果的临床分析[J].中国皮肤性病学杂志,2001,15(5):317-318
[13] Funnyé AS;Akhtar AJ. Syphilis and human immunodeficiency virus co-infection[J]. J Natl Med Assoc, 2003,95(5):363-382
[14] Smith NH, Musher DM, Huang DB, et al. response of HIV-infected patients with asymptomatic syphilis to intensive intramuscular therapy with ceftriaxone or procaine penicillin[J].Int J STD AIDS, 2004,15(5),328-332
[15] Shetsiruli,-L-T, Gogebashvili,-N-V, Toniia,-K-S, et al,immunologic aspects of the pathogenesis of latent and seroresistant forms of syphilis[J].Vestn-Dermatol-Venero, 1989,(5):55-58
[16] K.A. Yuldashev, K.M. Abzoirov, some immunological indicators in seroresistant syphilis[J].J Eur Acad Dermatol Venerol,2004,18(s2):486
[17] 杨日东,蔡川川,田广南,等.血清固定梅毒患者细胞免疫研究[J].中国实验诊断学,2004,8(5):477-479
[18] Kaminski,-A, Gede,-K, Owsinska,-H, et al. results of the treatment of neurosyphilis and seroresistant latent syphilis by intravenous administration of potassium salt of penicillin G[J]. Przegl-Dermatol,1989,76(3):256-261
[19] 昊建诚,林品东,陈天明,等。碘化钾治疗梅毒血清固定疗效观察[J].河北医学,2004,10(10):894-895
[20] 程喜平,雷公藤、强的松对 RPR、TPPA 滴度变化的影响[J].岭南皮肤性病科杂志,2002,9(4):241-244
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