基于脉动消元原理的磷酸盐分光光度检测系统研制
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摘要
水体中磷酸盐检测在环境监测中有重要意义。分光光度法作为应用最广泛的磷酸盐实验室检测方法难以满足现场快速、批量、准确检测需求,自动分析技术的引入,使得经典分光光度法在自动批量检测技术上有了很大改进。但是在水体中磷酸盐长期在线或原位监测中,分光光度系统的光源和传感器漂移、透光窗口的雾气及有色络合物附着污染等干扰都将导致测量误差。虽然已经有学者针对上述问题提出了一些解决方案,但是这些方案并未涉及抗干扰检测原理的创新,因此也就无法从根本上改变仪器的抗干扰能力。因此随着环境监测等相关领域对长期在线或原位监测需求越来越大,传统经典分光光度法不能满足这些新的需求。
针对这种情况,论文根据传统经典分光光度检测方法,创新性的提出了脉动消元分光光度检测原理,并根据该原理自主设计了脉动消元检测装置,结合自动化分析技术,提出一种适用于水体中磷酸盐长期在线或原位监测,在保证检测精度和稳定性的基础上能消除干扰的检测系统。本论文的主要工作内容和创新点为：
1、首次提出了脉动消元分光光度检测原理。在全面分析影响传统经典分光光度检测方法在水体中磷酸盐长期在线或原位监测应用中的干扰因素的基础上,深入研究差分技术原理,通过建立磷酸盐分光光度检测的干扰模型和数学分析,得出了能从本质上解决上述干扰的脉动消元原理。
2、根据脉动消元原理设计了脉动消元检测装置。脉动消元检测装置的设计实现了模块化、小型化、在线化的特点。机械设计采用恒压变光程技术,该恒压变光程结构配合步进电机、机械丝杆、检测容腔、传感器等部件,配合软硬件控制,实现检测过程中自动实时调节检测容腔内样品溶液的光程。实验结果表明,该装置的吸光度最大变化值为2%,达到了光源的最佳稳定性。而在同一光程不同检测过程和同一检测过程不同光程这两种情况下,吸光度最大变化值为0.01,为光源和检测器本身的精度。
3、研制了基于脉动消元检测装置的磷酸盐检测系统,并针对实际样品进行了实验验证。与传统经典分光光度测量方法的对比实验结果表明两者的吸光度检测精度同为0.01,而长期监测对比实验结果表明脉动消元检测系统的稳定性比传统检测提高了10倍。
因此本文研制的基于脉动消元原理的磷酸盐监测系统与传统仪器相比,不仅实现了长期原位在线自动监测,而且在保证检测精度和稳定性的基础上,大大提升了系统在实际应用中的抗干扰能力,具有很高的实用价值和很好的应用前景。该检测系统同时能满足水体中其他基于分光光度法的营养盐等相关长期检测需要,因既能应用于海洋领域,又能推广到相关的环境监测、工业过程检测等领域,从而对光学分析仪器科学技术的发展做出较大的贡献。
The detection of phosphate in water is an important section in environmental monitoring. Though most widely used in laboratory, the spectrophotometry is difficult to meet the requirements of on site, on speed, batch and accuracy. The introduction of automatic analysis technology enables great improvement of traditional spectrophotometry in automated batch detection. Further, during the long-term online or in situ monitoring of phosphate in water, measurement errors are caused by various interferences, including drift of spectrophotometric light sources and sensors, mist on the light window or staining by colored complex. As the existing solutions to address the problem have not overcome the interference essentially, the practical application of spectrophotometry, especially in the long-term online or in situ monitoring of phosphate in water, still cannot meet the research or practical demands.
According to such a status, based on the classic spectrophotometric detection, this paper proposes an innovative pulsating variable optical path length spectrophotometric detection method, and designs a pulsating variation of optical path length spectrophotometric detection device accordingly. Combined with automated analysis technology, it is a measurement system that can be used in the long-term online or in situ monitoring of phosphate in water to eliminate interference while ensuring measuring accuracy and stability. The content and innovations of this paper mainly include:
1. A pulsating variable optical pathlength spectrophotometric detection method is proposed. Based on a comprehensive analysis of the interfering factors existing in the long-term online or in situ monitoring of phosphate in the water using traditional spectrophotometric detetion and an in-depth study of differential technology, we construct an interference model and conduct a mathematical analysis to put forward the pulse variable optical path length spectrophotometric detection method that is able to eliminate all the above-mentioned interferences.
2. A pulsating variable optical path length spectrophotometric detection device is designed. This modular and miniaturized device can provide on-site and online measurement. Using constant-pressure variable optical path method, the main structure, together with stepper motor, machine screw, detection chamber, sensors and other components, can automatically adjust the optical path of the solution to be tested in the detection chamber through software control during the measuring process.The experimental results show that the biggest change of the absorbance is2%which achieves the best stability of the light source.
3. A phosphate detection system is developed based on the pulsating variable optical path length spectrophotometric device and experimental verification is carried out using actual samples. Comparison of experimental results with the classical spectrophotometic method show that both the absorbance accuracy is the same ad0.01, while the long-term monitoring results show that the stability of the pulsating variable optical path length sepctrophotometric device is10times higher than traditional ones.
Compared with traditional devices, this phosphate monitoring system based on pulse variable optical path length spectrophotometric detection method not only realizes the long-term on-line or in situ automatic monitoring, but also greatly enhances the anti-interference ability while guaranteeing the detection accuracy and stability. In sum, it is a system that can be widely used. Due to its ability to measure any nutrients that can be detected using spectrophotometry, it can be used in marine field as well as relevant environmental monitoring or industrial process detection, thereby making a great contribution to the development of optical analysis technologies and instruments.
针对这种情况,论文根据传统经典分光光度检测方法,创新性的提出了脉动消元分光光度检测原理,并根据该原理自主设计了脉动消元检测装置,结合自动化分析技术,提出一种适用于水体中磷酸盐长期在线或原位监测,在保证检测精度和稳定性的基础上能消除干扰的检测系统。本论文的主要工作内容和创新点为：
1、首次提出了脉动消元分光光度检测原理。在全面分析影响传统经典分光光度检测方法在水体中磷酸盐长期在线或原位监测应用中的干扰因素的基础上,深入研究差分技术原理,通过建立磷酸盐分光光度检测的干扰模型和数学分析,得出了能从本质上解决上述干扰的脉动消元原理。
2、根据脉动消元原理设计了脉动消元检测装置。脉动消元检测装置的设计实现了模块化、小型化、在线化的特点。机械设计采用恒压变光程技术,该恒压变光程结构配合步进电机、机械丝杆、检测容腔、传感器等部件,配合软硬件控制,实现检测过程中自动实时调节检测容腔内样品溶液的光程。实验结果表明,该装置的吸光度最大变化值为2%,达到了光源的最佳稳定性。而在同一光程不同检测过程和同一检测过程不同光程这两种情况下,吸光度最大变化值为0.01,为光源和检测器本身的精度。
3、研制了基于脉动消元检测装置的磷酸盐检测系统,并针对实际样品进行了实验验证。与传统经典分光光度测量方法的对比实验结果表明两者的吸光度检测精度同为0.01,而长期监测对比实验结果表明脉动消元检测系统的稳定性比传统检测提高了10倍。
因此本文研制的基于脉动消元原理的磷酸盐监测系统与传统仪器相比,不仅实现了长期原位在线自动监测,而且在保证检测精度和稳定性的基础上,大大提升了系统在实际应用中的抗干扰能力,具有很高的实用价值和很好的应用前景。该检测系统同时能满足水体中其他基于分光光度法的营养盐等相关长期检测需要,因既能应用于海洋领域,又能推广到相关的环境监测、工业过程检测等领域,从而对光学分析仪器科学技术的发展做出较大的贡献。
The detection of phosphate in water is an important section in environmental monitoring. Though most widely used in laboratory, the spectrophotometry is difficult to meet the requirements of on site, on speed, batch and accuracy. The introduction of automatic analysis technology enables great improvement of traditional spectrophotometry in automated batch detection. Further, during the long-term online or in situ monitoring of phosphate in water, measurement errors are caused by various interferences, including drift of spectrophotometric light sources and sensors, mist on the light window or staining by colored complex. As the existing solutions to address the problem have not overcome the interference essentially, the practical application of spectrophotometry, especially in the long-term online or in situ monitoring of phosphate in water, still cannot meet the research or practical demands.
According to such a status, based on the classic spectrophotometric detection, this paper proposes an innovative pulsating variable optical path length spectrophotometric detection method, and designs a pulsating variation of optical path length spectrophotometric detection device accordingly. Combined with automated analysis technology, it is a measurement system that can be used in the long-term online or in situ monitoring of phosphate in water to eliminate interference while ensuring measuring accuracy and stability. The content and innovations of this paper mainly include:
1. A pulsating variable optical pathlength spectrophotometric detection method is proposed. Based on a comprehensive analysis of the interfering factors existing in the long-term online or in situ monitoring of phosphate in the water using traditional spectrophotometric detetion and an in-depth study of differential technology, we construct an interference model and conduct a mathematical analysis to put forward the pulse variable optical path length spectrophotometric detection method that is able to eliminate all the above-mentioned interferences.
2. A pulsating variable optical path length spectrophotometric detection device is designed. This modular and miniaturized device can provide on-site and online measurement. Using constant-pressure variable optical path method, the main structure, together with stepper motor, machine screw, detection chamber, sensors and other components, can automatically adjust the optical path of the solution to be tested in the detection chamber through software control during the measuring process.The experimental results show that the biggest change of the absorbance is2%which achieves the best stability of the light source.
3. A phosphate detection system is developed based on the pulsating variable optical path length spectrophotometric device and experimental verification is carried out using actual samples. Comparison of experimental results with the classical spectrophotometic method show that both the absorbance accuracy is the same ad0.01, while the long-term monitoring results show that the stability of the pulsating variable optical path length sepctrophotometric device is10times higher than traditional ones.
Compared with traditional devices, this phosphate monitoring system based on pulse variable optical path length spectrophotometric detection method not only realizes the long-term on-line or in situ automatic monitoring, but also greatly enhances the anti-interference ability while guaranteeing the detection accuracy and stability. In sum, it is a system that can be widely used. Due to its ability to measure any nutrients that can be detected using spectrophotometry, it can be used in marine field as well as relevant environmental monitoring or industrial process detection, thereby making a great contribution to the development of optical analysis technologies and instruments.
引文
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