湖南地区慢性HBV患者和HBV携带者MHC-Ⅰ类链相关基因A第五外显子微卫星多态性研究
|
摘要
目的了解湖南汉族人群中的慢性HBV患者、HBV携带者和健康对照人群中MICA外显子5的微卫星多态性(MICA—STR),比较分析其各基因型、等位基因频率以及表型频率的差异。
方法应用聚合酶链反应和基因扫描技术分别对108例慢性HBV患者、96例HBV携带者和142例健康对照者进行MICA基因第5外显子微卫星多态性分析;应用DNA测序分析对不同的基因型进行验证;应用PCR/SSP技术进行MICA*Del的检测;综合以上三项检测结果,确定MICA基因第5外显子基因型。
结果1.本次研究的三组中分别检出A4,A5,A5.1,A6,A9五种等位基因,在HBV携带组和健康对照组中分别检出1例MICA*Del基因。2.慢性HBV患者组MICA*A5.1/A9基因型频率(7/108)低于健康对照组(22/142),RR=0.378,P=0.028;慢性HBV患者组的MICA*A9等位基因频率(20/216)低于健康对照组(46/248),RR=0.526,P=0.023;慢性HBV患者组MICA*A9表型频率(18/108)低于健康对照组(45/142),RR=0.431,P=0.007。3.HBV携带组MICA*A5.1/A9基因型频率(4/96)低于健康对照组(22/142),RR=0.237,P=0.006;HBV携带组MICA*A9等位基因频率(18/192)低于健康对照组(46/284)RR=0.535,P=0.032;HBV携带组MICA*A9表型频率(17/96)低于健康对照组(45/142),RR=0.464,P=0.016。4.慢性HBV患者组与HBV携带者组间MICA基因第5外显子各基因型、等位基因频率、表型频率的差异均无统计学意义(P＞0.05)。
结论1.湖南地区汉族人群MICA-STR只检出A4,A5,A5.1,A6,A9五种等位基因,且以A5和A5.1为主。2.研究发现湖南地区汉族人群中HBV携带者和慢性HBV患者的MICA*A5.1/A9基因型频率低于健康对照组(P＜0.05),推测MICA*A5.1/A9基因型可能是抗HBV感染的一种保护性基因型。3.研究发现湖南地区汉族人群中HBV携带者和慢性HBV患者的MICA*A9等位基因频率和表型频率均低于健康对照组(P＜0.05),推测MICA*A9等位基因可能是抗HBV感染的一种保护性等位基因。4.湖南地区汉族人群HBV携带者和慢性HBV患者间的基因型频率、等位基因频率和表型频率差异均无统计学意义(P＞0.05)。
Objective To investigate the genetic polymorphism of microsatellite in theexon 5 of MICA gene(MICA-STR) in HBV carriers,chronic HBV infectious patients and healthy controls of Han population in Hunan Province and analyze the distinction of their genotypes, gene frequency and phenotypic frequency.
Methods The microsatellite polymorphism of MICA exon 5 in 108 chronicHBV infectious patients,96 HBV carriers and 142 healthy controls were analyzed using polymerase chain reaction(PCR) and genescanning technique. The genotypes were confirmed by DNA sequencing. .MICA gene deletion was detected byPCR-sequence specific priming(PCR/SSP). The genotypes of MICA exon 5 were determined according to the three detecting results.
Results 1. Five alleles of MICA-STR were detected in this study,which are A4,A5,A5.1,A6 and A9.One MICA*Del gene was detected in both HBV carrier group and healthy control group.2. MICA*A5.1/A9 was observed at significantlylower frequency in the chronic HBV patient group than in the healthy control group ( relative risk = 0.378, P = 0.028); MICA* A9 was present at significantly lower frequency in the chronic HBV patient group than in the healthy control group (RR = 0.526, P = 0. 023);Phenotypic frequency of MICA*A9 was significantly lower in chronic HBV patient group than in the healthy control group (RR = 0.431, P = 0. 007).3. MICA*A5.1/A9 was observed at signifieantly lower frequency in the HBV carrier group than in the healthy control group ( relative risk = 0.237, P = 0. 006); Gene frequency of MICA*A9 was signifieantly lower in the HBV carrier group than in the healthy control group (RR=0.535, P=0.032); Phenotypic frequency of MICA*A9 was significantly lower in the HBV carrier group than in the healthy control group (relative risk = 0.464, P = 0. 016).4. The genotypic frequency, allele frequency and phenotypic frequency of MICA exon 5 showed no statistically significant difference between the HBV carrier group and the chronic HBV patient group(P>0.05).
Conclusions 1.Five alleles of MICA-STR are detected in Hunan Hanpopulation,which are A4,A5,A5.1,A6 and A9.Among them,the A5 and A5.1 allele are more frequently observed.2.MICA*A5.1/A9 seems to be one of protective genotypes against HBV infection in that it is the first report that its genotypic frequency in HBV carriers and chronic HBV patients is significantly lower than in healthy controls in Hunan Han population.3. It is the first report that the allele frequency and phenotypic frequency of MICA* A9 are significantly lower in HBV carriers and chronic HBV patients than in healthy controls in Hunan Han population,so it is presumed that MICA* A9 is one of protective genes against HBV infection.4. The genotypic frequency, allele frequency and phenotypic frequency show no statistically significant difference between the HBV carriers and the chronic HBV patients in Hunan Han population.
方法应用聚合酶链反应和基因扫描技术分别对108例慢性HBV患者、96例HBV携带者和142例健康对照者进行MICA基因第5外显子微卫星多态性分析;应用DNA测序分析对不同的基因型进行验证;应用PCR/SSP技术进行MICA*Del的检测;综合以上三项检测结果,确定MICA基因第5外显子基因型。
结果1.本次研究的三组中分别检出A4,A5,A5.1,A6,A9五种等位基因,在HBV携带组和健康对照组中分别检出1例MICA*Del基因。2.慢性HBV患者组MICA*A5.1/A9基因型频率(7/108)低于健康对照组(22/142),RR=0.378,P=0.028;慢性HBV患者组的MICA*A9等位基因频率(20/216)低于健康对照组(46/248),RR=0.526,P=0.023;慢性HBV患者组MICA*A9表型频率(18/108)低于健康对照组(45/142),RR=0.431,P=0.007。3.HBV携带组MICA*A5.1/A9基因型频率(4/96)低于健康对照组(22/142),RR=0.237,P=0.006;HBV携带组MICA*A9等位基因频率(18/192)低于健康对照组(46/284)RR=0.535,P=0.032;HBV携带组MICA*A9表型频率(17/96)低于健康对照组(45/142),RR=0.464,P=0.016。4.慢性HBV患者组与HBV携带者组间MICA基因第5外显子各基因型、等位基因频率、表型频率的差异均无统计学意义(P＞0.05)。
结论1.湖南地区汉族人群MICA-STR只检出A4,A5,A5.1,A6,A9五种等位基因,且以A5和A5.1为主。2.研究发现湖南地区汉族人群中HBV携带者和慢性HBV患者的MICA*A5.1/A9基因型频率低于健康对照组(P＜0.05),推测MICA*A5.1/A9基因型可能是抗HBV感染的一种保护性基因型。3.研究发现湖南地区汉族人群中HBV携带者和慢性HBV患者的MICA*A9等位基因频率和表型频率均低于健康对照组(P＜0.05),推测MICA*A9等位基因可能是抗HBV感染的一种保护性等位基因。4.湖南地区汉族人群HBV携带者和慢性HBV患者间的基因型频率、等位基因频率和表型频率差异均无统计学意义(P＞0.05)。
Objective To investigate the genetic polymorphism of microsatellite in theexon 5 of MICA gene(MICA-STR) in HBV carriers,chronic HBV infectious patients and healthy controls of Han population in Hunan Province and analyze the distinction of their genotypes, gene frequency and phenotypic frequency.
Methods The microsatellite polymorphism of MICA exon 5 in 108 chronicHBV infectious patients,96 HBV carriers and 142 healthy controls were analyzed using polymerase chain reaction(PCR) and genescanning technique. The genotypes were confirmed by DNA sequencing. .MICA gene deletion was detected byPCR-sequence specific priming(PCR/SSP). The genotypes of MICA exon 5 were determined according to the three detecting results.
Results 1. Five alleles of MICA-STR were detected in this study,which are A4,A5,A5.1,A6 and A9.One MICA*Del gene was detected in both HBV carrier group and healthy control group.2. MICA*A5.1/A9 was observed at significantlylower frequency in the chronic HBV patient group than in the healthy control group ( relative risk = 0.378, P = 0.028); MICA* A9 was present at significantly lower frequency in the chronic HBV patient group than in the healthy control group (RR = 0.526, P = 0. 023);Phenotypic frequency of MICA*A9 was significantly lower in chronic HBV patient group than in the healthy control group (RR = 0.431, P = 0. 007).3. MICA*A5.1/A9 was observed at signifieantly lower frequency in the HBV carrier group than in the healthy control group ( relative risk = 0.237, P = 0. 006); Gene frequency of MICA*A9 was signifieantly lower in the HBV carrier group than in the healthy control group (RR=0.535, P=0.032); Phenotypic frequency of MICA*A9 was significantly lower in the HBV carrier group than in the healthy control group (relative risk = 0.464, P = 0. 016).4. The genotypic frequency, allele frequency and phenotypic frequency of MICA exon 5 showed no statistically significant difference between the HBV carrier group and the chronic HBV patient group(P>0.05).
Conclusions 1.Five alleles of MICA-STR are detected in Hunan Hanpopulation,which are A4,A5,A5.1,A6 and A9.Among them,the A5 and A5.1 allele are more frequently observed.2.MICA*A5.1/A9 seems to be one of protective genotypes against HBV infection in that it is the first report that its genotypic frequency in HBV carriers and chronic HBV patients is significantly lower than in healthy controls in Hunan Han population.3. It is the first report that the allele frequency and phenotypic frequency of MICA* A9 are significantly lower in HBV carriers and chronic HBV patients than in healthy controls in Hunan Han population,so it is presumed that MICA* A9 is one of protective genes against HBV infection.4. The genotypic frequency, allele frequency and phenotypic frequency show no statistically significant difference between the HBV carriers and the chronic HBV patients in Hunan Han population.
引文
1.中华人民共和国卫生部公报,2009,第3期.
2.Hilleman MR.Overview of the pathogenesis,prophylaxis and therapeusis of viral hepatitis B,with focus on reduction to pratical applications[J].Vaccine,2001,19(15-16):1837-1848.
3.Poovorawan Y,Chatchatee P,Chongsrisawat V.Epidemiology and prophylaxis of viral hepatitis:A global perspective[J].J Gastroenterol Hepatol,2002,17(suppl):S155-166.
4.Kenny-Walsh E.Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin.Irish Hepatology Research Group[J].N Engl J Med,1999,340(16):1228-1233.
5.Hohler T,Gerken G;Notghi A,et al.HLA-DRBI~*1301 and~*1302 protect against chronic hepatitis B[J].J Hepatol,1997,26(3):503-507.
6.Thio CL,Gao X,Goedert JJ et al.HLA-Cw~*04 and hepatitis C virus persistence[J].J Viro1,2002,76(10):4792-4797.
7.Almarri A,Batchelor JR.HLA and hepatitis B infection[J].Lancet,1994,344(8931):1194- 1195.
8.陈湖光,蒋国法,孟雪琴,等.乙型肝炎病毒感染者人类白细胞抗原的检测[J].中华传染病杂志,2002,20(3):164-167.
9.Li P,Willie ST,Bauer S,et al.Crystal structure of the MHC class I homolog MICA,a gammadelta T cell ligand[J].Immunity,1999,10(5):577-584.
10.Fodil N,Laloux L,Wanner V,et al.Allelic repertoire of the human MHC class I MICA gene[J].Immunogenetics,1996,44(5):351-357.
11.Bauer S,Groh V,Wu J,et al.Activation of NK cells and T cells by NKG2D,a receptor for stress-inducible MICA[J].Science.1999,285(5428):727-729.
12.Venkataraman GM,Suciu D,Groh V,et al..Promoter region architecture and transcriptional regulation of the genes for the MHC class I-related chain A and B ligands ofNKG2D[J].J Immunol.2007,178(2):961-969.
13.Bauer S,Groh V,Wu J,et al.Activation of NK cells and T cells by NKG2D,a receptor for stress-inducible MICA[J].Science,1999,285(5428):727-729.
14.Karacki PS,Gao X,Thio CL,et al.MICA and recovery from hepatitis C virus and hepatitis B virus infections[J].Genes and Immunity,2004,5(4):261-266.
15.Mizuki N,Ota M,Kimura M,et al.Triplet repeat polymorphism in the transmembrane region of the MICA gene:A strong association of six GCT repetitions with Behcet's disease[J].Proc Natl Acad Sci USA 1997,94:1298-1303
16.Collins RW,Stephens HA,Clare MA,et al.High resolution molecular phototyping of MICA and MICB alleles using sequence specific primers.Hum Immunol,2002,63(9):783-794.
17.肖翠英,张思仲,程璐,等.中国四个人群中M ICA基因多态性研究[J].中华医学遗传学杂志,2000,17:424-428.
18.中国南方汉族人群MICA-STR的遗传多态性[J].中华微生物学与免疫学杂志,2005,25(6):446-449.
19.Perez-Rodriguez M,Corell A,Arguello JR,et al.A new MICA allele with ten alanine residues in the exon 5 microsatellite[J].Tissue Antigens,2000,55(2):162-165.
20.MICA AS:a new allele within MHC class I chain-related A transmembrane region with eight GCT repeats[J].Hum Immunol,2006,67(12):1005-1007.
21.Komatsu-Wakui M,Tokunaga K,Ishikawa Y,et al.MIC-A polymorphism in Japanese and a MIC-A-MIC-B null haplotype.Immunogenetics,1999,49(728):620-628.
22.Tian W,Boggs DA,Uko G,et al.MICA,HLA-B haplotypic variation in five population groups of sub2Saharan African ancestry.Genes Immun,2003,4(7):500-505.
23.Aida K,Russomando G,Kikuchi M,et al.High frequency of MIC null haplotype (HLA-B48-MICA-del-MICB*0107 N) in the Angaite Amerindian community in Paraguay.Immunogenetics,2002,54(6):439-441.
24.Komatsu-Wakui M,Tokunaga K,Ishikawa Y,et al.Wide distribution of the MICA-MICB null haplotype in East Asians.Tissue Antigens,2001,57(1):1-8.
25.Mizuki N,Ota M,Kimura M,et al.Triplet repeat polymorphism in the transmembrane region of the MICA gene;a strong association of six GCT repetitions with Behcet disease[J].Proc natl Acad Sci USA,1997,94(4):1298-1303.
26.Ota M,Mizuki N,Katsuyama Y,et al.The critical region for Behcet disease in the human major histocompatibility complex is reduced to a 46kb segment centromeric of HLA-B,by association analysis using refinedmicrosatellite mapping[J].Am J Hum Genet,1999,64(5):1406-1410.
27.Kawabata Y,lkegami H,Kawaguchi Y,et al.Age-related association of MHC class I chain-related gene A(MICA) with type 1(insulin-dependent)diabetes mellitus[J].Hum Immunology,2000,61(6):624-629.
28.Park Y,Lee H,Sanjeevi C B,et al.MICA Polymorphism is association of type 1diabetes in the Korean population[J].Diabetes Care,2001,24(1):33-38.
29.Tica V,Nikitina-Zake L,Donadi E,et al.MIC-A genotypes 4/5.1 and 9/9 are positively associated with type 1 diabetes mellitus in Brazilian population[J].Ann N Y Acad Sci,2003,1005:310-313.
30.Lopez-Vazquez A,Rodrigo L,Mina-Blanco A,et al.Extended human leukocyte antigen haplotype EH18.1 influences progression to hepatocellular carcinoma in patients with hepatitis C virus infection[J].Infect Dis,2004,189(6):957-963.
31.Liu C J,Lee Y J,Chang KW,et al.Polymorphism of the MICA gene and risk for oral submucous fibrosis[J].J Oral Pathol Med,2004,33(1):1-6.
32.黄柏胜,罗奇志,李立新,等.湖南汉族肾移植受者MICA*008/5.1基因与HCMV 感染的关系[J].中南大学学报(医学版),2006,31(4):479-482.
33.Karacki PS,Gao X,Thio CL,et al.MICA and recovery from hepatitis C virus and hepatitis B virus infections[J].Genes and Immunity,2004,5(4):261-266.
34.Jiang K,Zhong B,Gilvary DL,et al.Pivotal role of phosphoinositide-3 kinase in regulation of cytotoxicity in natural killer cells[J].Nat Immunol,2000,1(5):419-425.
35.陈敏,彭明利,石庆风,等.乙型肝炎病毒全基因转染L02细胞模型的建立及其HLA-A,B,C和MHCI类链相关基因A/B分子的表达[J].中华肝脏病杂志,2007,15(6):417-421.
36.Tang KF,Chen M,Xie J,et al.Inhibition of hepatitis B virus replication by small Interference RNA induces expression of MICA in HepG2.2.15 cells[J].Med Microbiol Immunol,2009,198(1):27-32.
1.Bahram S.MIC genes:from genetics to biology[J].Adv Immunol,2000,76(1):1-60.
2.Bahram S,Mizuki N,Inoko H,et al.Nucleotide sequence of the human MHC class Ⅰ MICA gene[J].Immunogenetics,1996,44(1):80-81.
3.Bahram S,Bresnahan M,Geraghty DE,et al.A second lineage of mammalian major histoeompatibility complex class Ⅰ genes[J].Proc Natl Acad Sci USA,1994,91(14):6259-6263.
4.Li P,Willie ST,Bauer S,et al.Crystal structure of the MHC class Ⅰ homolog MICA,a gamma delta T cell ligand[J].Immunity,1999,10(5):577-584.
5.Kasahara M,Watanabe Y,Sumasu M,et al.A family of MHC class Ⅰ like genes located in the vicinity of the mouse leukocyte receptor complex[J].Immun- ology,2002,99(21):13687-13692.
6.Hankey K G,Drachenberg C B,Papadimitdou J C,et al.MIC expression in renal and pancreatic allografts[J].Transplantation,2002,73(2):304-306.
7.Groh V,Rhinehart R,Secrist H,et al.Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB[J].Proc N atl A cad Sci USA,1999,96(12):6879-6884.
8.Groh V,S teinle A,Bauer S,et al.Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells[J].Science,1998,279(5357):1737-1740.
9.Groh V,Rhinehart R,Randolph2Habecker J,et al.Costimutation of CD8 +alphabeta T cells by NKG2D via engagement by MIC induced on virus infe-cted cells[j].Nat Immunol,2001,2(3):255-260.
10.Jiang K,Zhong B,Gilvary DL,et al.Pivotal role of phosphoinositide-3 kinase in regulation of cytotoxicity in natural killer cells[J].Nat Immunol,2000,1(5):419-425.
11.Perez-Rodriguez M,Corell A,Arguello JR,et al.A new MICA allele with ten alanine residues in the exon 5 microsatellite[J].Tissue Antigens,2000,55(2):162-165.
12.MICA A8:a new allele within MHC class Ⅰ chain-related A transmembrane region with eight GCT repeats[J].Hum Immunol,2006,67(12):1005 - 1007.
13.Petersdorf EW,Shuler KB,Longton G,et al.Population study of allelic versity in the human MHC class Ⅰ realted MICA-A gene[J].Immunogenetics,1999,49(7-8):605-612.
14.Katsuyama Y,Ota M,Ando H,et al.Sequencing based typing for genetic polymorphisms in exons,2,3 and 4 of the MICA gene[J].Tissue Antigens,1999,54(2)i178-184.
15.龚卫娟,范丽安,杨珏琴,等.三个群体MICA基因外显子2、3和4的多态性研究[J].中华医学遗传学杂志,2002,19(4):336-339.
16.Fodil N,Pellet P,Laloux L,et al.MICA haplotypicdiversity[J].Immunogenetics,1999,49(6):557-560.
17.Groh V,S teinle A,Bauer S,et al.Recognition of stress-induced MHC molecules by intestinal epithelial gamma delta T cells[J].Science,1998,279(5357):1737-1740.
18.Komatsu-Wakui M TokunagaK,IshikawaY,et al.MIC-A polymorphism in Japanese and a MIC-A-MIC-B null haplotype[J].Immunogenetics,1999,49(7-8):620-628.
19.Mizuki N,Ota M,Kimura M,et al.Triplet repeat polymorphism in the transmembrane region of the MICA gene;a strong association of six GCT repetitions with Behcet disease[J].Proc natl Acad Sci USA,1997,94(4):1298-1303.
20.Wallace GR,Verity DH,Delamaine LJ et al.MIC-A allele profiles and HLA class associations in Beh(?)et's disease[J].Immunogenetics,1999,49(7-8):613-617.
21.Goto K,Ota M,Ohno S,et al.MICA gene and ankylosing spondylitis:linkage analysis via a transmembrane encoded triplet repeat polymorphism[J].Tissus Antigens,1997,49(5):503-507.
22.Yabuki K,et al.Triplet repeat polymorphism in the MICA gene in HLA-B27position and negative cancasicm patients with anklosing spondylitis[J].Human Immunology,1999,60(1):83-86.
23.Kawabata Y,lkegami H,Kawaguchi Y,et al.Age-related association of MHC class Ⅰ chain-related gene A(MICA) with type 1(insulin-dependent)diabetes mellitus[J].Hum Immunology,2000,61(6):624-629.
24.Park Y,Lee H,Sanjeevi C B,et al.MICA Polymorphism is association of type 1diabetes in the Korean population[J].Diabetes Care,2001,24(1):33-38.
25.Tica V,Nikitina-Zake L,Donadi E,et al.MIC-A genotypes 4/5.1 and 9/9 are positively associated with type 1 diabetes mellitus in Brazilian population[J].Ann N Y Acad Sci,2003,1005:310-313.
26.Ochard TR,Dhar A,Simmons JD,et al.MHC class Ⅰ chain-like gene A (MICA) and its associations with inflammatory bowel disease and peripheral arthropathy[J].Clin Exp Immunol,2001,126(3):437-440.
27.Lopez-Vazquez A,Rodrigo L,Mina-Blanco A,et al.Extended human leukocyte antigen haplotype EH18.1 influences progression to hepatocellular carcinoma in patients with hepatitis C virus infection[J].Infect Dis,2004,189(6):957-963.
28.Lopez-Vazquez A,Fuentes D,Rodrigo L,et al.MHC class Ⅰ region plays a role in the development of diverse clinical forms of celiac disease in a Saharawi population[J].AmJ Gastroenterol,2004,99(4):662-667.
29.Karacki PS,Gao X,Thio CL,et al.MICA and recovery from hepatitis C virus and hepatitis B virus infections[J].Genes and Immunity,2004,5(4):261-266.
30.Liu CJ,Lee YJ,Chang KW,et al.Polymorphism of the MICA gene and risk for oral submucous fibrosis[J].J Oral Pathol Med,2004,33(1):1-6.
31.班贵宏,褚嘉佑,冒长峙,等.MICA基因与系统性红斑狼疮关系的研究[J].中华医学遗传学杂志,2002,19(4):298-301.
32.田伟,罗奇志,李立新,等.湖南地区鼻咽癌MHC-I类链相关基因A第5外显子微卫星多态性研究[J].中华医学遗传学杂志,2005,22(3):309-312.
33.黄柏胜,罗奇志,李立新,等.湖南汉族肾移植受者MICA*008/5.1基因与HCMV感染的关系[J].中南大学学报(医学版),2006,31(4):479-482.
34.李立新,田伟,罗奇志,等.湖南地区白血病MHC-Ⅰ类链相关基因A微卫星多态性分析[J].中国免疫学杂志,2007,23(2):129-133.
35.McDermott A.B,Cohen S.B,Zuckerman J.N,et al.Human leucocyte antigens influence the immune response to a pre-s/s hepatitis B Vaccine.Vaccine,1999,17:330-339.
36.Desombere I,Willems A,Leroux - Roels G,et al.Response to hepatitis B vaccine:multiple HLA genes are involved[J].Tissue Antigens,1998,51(6):593-604.
37.刘蓬勃,徐慧文,王学良,等.乙肝疫苗接种无、弱应答与遗传因素关系[J].第 四军医大学学报,2000;21(1):30-33.
38.Thio CL,Carrington M,Marti D,et al.Class Ⅱ HLA alleles and hepatitis B virus persistence in African Americans[J].J Infect Dis,1999,179(4):1004-1006.
39.蒋业贵,王宇明.乙型肝炎患者人类白细胞抗原-DRB1、-DQA1、-DQB1等位基因多态性分析[J].中华流行病学杂志,2004,25(4):337-340.
40.Han Y.-N.,Yang J.-L.,Zhen S.-G.,Tang Q.and Zhu W.Relationship of human leukocyte antigen class Ⅱ genes with the susceptibility hepatitis to B virus infection and the response to interferon in HBV-infected patients[J].World Journal of Gastroenterology,2005,11(36):5721-5724.
41.邓小梅,王金凤,邹雄,等.乙型肝炎病毒感染与宿主淋巴细胞HLA-Ⅰ类分子关系的研究[J].山东大学学报(医学版),2006,12(44):1258-1264.
42.Vivier E,Tomasello E and Paul P.Lymphocyte activation via NKG2D:towards a new paradigm in immune recognition[J]Curt Opin in Immunol.2002,14(3):306-311.
43.陈敏,彭明利,石庆凤,等.乙型肝炎病毒全基因转染L02细胞模型的建立及其HLA-A,B,C和MHCI类链相关基因A/B分子的表达[J].中华肝脏病杂志,2007,15(6):417-421.
44.Yongyan Chen,Min Cheng,Zhigang Tian.Hepatitis B Virus Down-Regulates Expressions of MHC Class Ⅰ Molecules on Hepatoplastoma Cell Line[J].Cellular Molecular Immunology,2006,5(3):373-378.
45.Tang KF,Chen M,Xie J,et al.Inhibition of hepatitis B vires replication by small Interference RNA induces expression of MICA in HepG2.2.15 cells[J].Med Microbiol Immunol,2009,198(1):27-32.
2.Hilleman MR.Overview of the pathogenesis,prophylaxis and therapeusis of viral hepatitis B,with focus on reduction to pratical applications[J].Vaccine,2001,19(15-16):1837-1848.
3.Poovorawan Y,Chatchatee P,Chongsrisawat V.Epidemiology and prophylaxis of viral hepatitis:A global perspective[J].J Gastroenterol Hepatol,2002,17(suppl):S155-166.
4.Kenny-Walsh E.Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin.Irish Hepatology Research Group[J].N Engl J Med,1999,340(16):1228-1233.
5.Hohler T,Gerken G;Notghi A,et al.HLA-DRBI~*1301 and~*1302 protect against chronic hepatitis B[J].J Hepatol,1997,26(3):503-507.
6.Thio CL,Gao X,Goedert JJ et al.HLA-Cw~*04 and hepatitis C virus persistence[J].J Viro1,2002,76(10):4792-4797.
7.Almarri A,Batchelor JR.HLA and hepatitis B infection[J].Lancet,1994,344(8931):1194- 1195.
8.陈湖光,蒋国法,孟雪琴,等.乙型肝炎病毒感染者人类白细胞抗原的检测[J].中华传染病杂志,2002,20(3):164-167.
9.Li P,Willie ST,Bauer S,et al.Crystal structure of the MHC class I homolog MICA,a gammadelta T cell ligand[J].Immunity,1999,10(5):577-584.
10.Fodil N,Laloux L,Wanner V,et al.Allelic repertoire of the human MHC class I MICA gene[J].Immunogenetics,1996,44(5):351-357.
11.Bauer S,Groh V,Wu J,et al.Activation of NK cells and T cells by NKG2D,a receptor for stress-inducible MICA[J].Science.1999,285(5428):727-729.
12.Venkataraman GM,Suciu D,Groh V,et al..Promoter region architecture and transcriptional regulation of the genes for the MHC class I-related chain A and B ligands ofNKG2D[J].J Immunol.2007,178(2):961-969.
13.Bauer S,Groh V,Wu J,et al.Activation of NK cells and T cells by NKG2D,a receptor for stress-inducible MICA[J].Science,1999,285(5428):727-729.
14.Karacki PS,Gao X,Thio CL,et al.MICA and recovery from hepatitis C virus and hepatitis B virus infections[J].Genes and Immunity,2004,5(4):261-266.
15.Mizuki N,Ota M,Kimura M,et al.Triplet repeat polymorphism in the transmembrane region of the MICA gene:A strong association of six GCT repetitions with Behcet's disease[J].Proc Natl Acad Sci USA 1997,94:1298-1303
16.Collins RW,Stephens HA,Clare MA,et al.High resolution molecular phototyping of MICA and MICB alleles using sequence specific primers.Hum Immunol,2002,63(9):783-794.
17.肖翠英,张思仲,程璐,等.中国四个人群中M ICA基因多态性研究[J].中华医学遗传学杂志,2000,17:424-428.
18.中国南方汉族人群MICA-STR的遗传多态性[J].中华微生物学与免疫学杂志,2005,25(6):446-449.
19.Perez-Rodriguez M,Corell A,Arguello JR,et al.A new MICA allele with ten alanine residues in the exon 5 microsatellite[J].Tissue Antigens,2000,55(2):162-165.
20.MICA AS:a new allele within MHC class I chain-related A transmembrane region with eight GCT repeats[J].Hum Immunol,2006,67(12):1005-1007.
21.Komatsu-Wakui M,Tokunaga K,Ishikawa Y,et al.MIC-A polymorphism in Japanese and a MIC-A-MIC-B null haplotype.Immunogenetics,1999,49(728):620-628.
22.Tian W,Boggs DA,Uko G,et al.MICA,HLA-B haplotypic variation in five population groups of sub2Saharan African ancestry.Genes Immun,2003,4(7):500-505.
23.Aida K,Russomando G,Kikuchi M,et al.High frequency of MIC null haplotype (HLA-B48-MICA-del-MICB*0107 N) in the Angaite Amerindian community in Paraguay.Immunogenetics,2002,54(6):439-441.
24.Komatsu-Wakui M,Tokunaga K,Ishikawa Y,et al.Wide distribution of the MICA-MICB null haplotype in East Asians.Tissue Antigens,2001,57(1):1-8.
25.Mizuki N,Ota M,Kimura M,et al.Triplet repeat polymorphism in the transmembrane region of the MICA gene;a strong association of six GCT repetitions with Behcet disease[J].Proc natl Acad Sci USA,1997,94(4):1298-1303.
26.Ota M,Mizuki N,Katsuyama Y,et al.The critical region for Behcet disease in the human major histocompatibility complex is reduced to a 46kb segment centromeric of HLA-B,by association analysis using refinedmicrosatellite mapping[J].Am J Hum Genet,1999,64(5):1406-1410.
27.Kawabata Y,lkegami H,Kawaguchi Y,et al.Age-related association of MHC class I chain-related gene A(MICA) with type 1(insulin-dependent)diabetes mellitus[J].Hum Immunology,2000,61(6):624-629.
28.Park Y,Lee H,Sanjeevi C B,et al.MICA Polymorphism is association of type 1diabetes in the Korean population[J].Diabetes Care,2001,24(1):33-38.
29.Tica V,Nikitina-Zake L,Donadi E,et al.MIC-A genotypes 4/5.1 and 9/9 are positively associated with type 1 diabetes mellitus in Brazilian population[J].Ann N Y Acad Sci,2003,1005:310-313.
30.Lopez-Vazquez A,Rodrigo L,Mina-Blanco A,et al.Extended human leukocyte antigen haplotype EH18.1 influences progression to hepatocellular carcinoma in patients with hepatitis C virus infection[J].Infect Dis,2004,189(6):957-963.
31.Liu C J,Lee Y J,Chang KW,et al.Polymorphism of the MICA gene and risk for oral submucous fibrosis[J].J Oral Pathol Med,2004,33(1):1-6.
32.黄柏胜,罗奇志,李立新,等.湖南汉族肾移植受者MICA*008/5.1基因与HCMV 感染的关系[J].中南大学学报(医学版),2006,31(4):479-482.
33.Karacki PS,Gao X,Thio CL,et al.MICA and recovery from hepatitis C virus and hepatitis B virus infections[J].Genes and Immunity,2004,5(4):261-266.
34.Jiang K,Zhong B,Gilvary DL,et al.Pivotal role of phosphoinositide-3 kinase in regulation of cytotoxicity in natural killer cells[J].Nat Immunol,2000,1(5):419-425.
35.陈敏,彭明利,石庆风,等.乙型肝炎病毒全基因转染L02细胞模型的建立及其HLA-A,B,C和MHCI类链相关基因A/B分子的表达[J].中华肝脏病杂志,2007,15(6):417-421.
36.Tang KF,Chen M,Xie J,et al.Inhibition of hepatitis B virus replication by small Interference RNA induces expression of MICA in HepG2.2.15 cells[J].Med Microbiol Immunol,2009,198(1):27-32.
1.Bahram S.MIC genes:from genetics to biology[J].Adv Immunol,2000,76(1):1-60.
2.Bahram S,Mizuki N,Inoko H,et al.Nucleotide sequence of the human MHC class Ⅰ MICA gene[J].Immunogenetics,1996,44(1):80-81.
3.Bahram S,Bresnahan M,Geraghty DE,et al.A second lineage of mammalian major histoeompatibility complex class Ⅰ genes[J].Proc Natl Acad Sci USA,1994,91(14):6259-6263.
4.Li P,Willie ST,Bauer S,et al.Crystal structure of the MHC class Ⅰ homolog MICA,a gamma delta T cell ligand[J].Immunity,1999,10(5):577-584.
5.Kasahara M,Watanabe Y,Sumasu M,et al.A family of MHC class Ⅰ like genes located in the vicinity of the mouse leukocyte receptor complex[J].Immun- ology,2002,99(21):13687-13692.
6.Hankey K G,Drachenberg C B,Papadimitdou J C,et al.MIC expression in renal and pancreatic allografts[J].Transplantation,2002,73(2):304-306.
7.Groh V,Rhinehart R,Secrist H,et al.Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB[J].Proc N atl A cad Sci USA,1999,96(12):6879-6884.
8.Groh V,S teinle A,Bauer S,et al.Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells[J].Science,1998,279(5357):1737-1740.
9.Groh V,Rhinehart R,Randolph2Habecker J,et al.Costimutation of CD8 +alphabeta T cells by NKG2D via engagement by MIC induced on virus infe-cted cells[j].Nat Immunol,2001,2(3):255-260.
10.Jiang K,Zhong B,Gilvary DL,et al.Pivotal role of phosphoinositide-3 kinase in regulation of cytotoxicity in natural killer cells[J].Nat Immunol,2000,1(5):419-425.
11.Perez-Rodriguez M,Corell A,Arguello JR,et al.A new MICA allele with ten alanine residues in the exon 5 microsatellite[J].Tissue Antigens,2000,55(2):162-165.
12.MICA A8:a new allele within MHC class Ⅰ chain-related A transmembrane region with eight GCT repeats[J].Hum Immunol,2006,67(12):1005 - 1007.
13.Petersdorf EW,Shuler KB,Longton G,et al.Population study of allelic versity in the human MHC class Ⅰ realted MICA-A gene[J].Immunogenetics,1999,49(7-8):605-612.
14.Katsuyama Y,Ota M,Ando H,et al.Sequencing based typing for genetic polymorphisms in exons,2,3 and 4 of the MICA gene[J].Tissue Antigens,1999,54(2)i178-184.
15.龚卫娟,范丽安,杨珏琴,等.三个群体MICA基因外显子2、3和4的多态性研究[J].中华医学遗传学杂志,2002,19(4):336-339.
16.Fodil N,Pellet P,Laloux L,et al.MICA haplotypicdiversity[J].Immunogenetics,1999,49(6):557-560.
17.Groh V,S teinle A,Bauer S,et al.Recognition of stress-induced MHC molecules by intestinal epithelial gamma delta T cells[J].Science,1998,279(5357):1737-1740.
18.Komatsu-Wakui M TokunagaK,IshikawaY,et al.MIC-A polymorphism in Japanese and a MIC-A-MIC-B null haplotype[J].Immunogenetics,1999,49(7-8):620-628.
19.Mizuki N,Ota M,Kimura M,et al.Triplet repeat polymorphism in the transmembrane region of the MICA gene;a strong association of six GCT repetitions with Behcet disease[J].Proc natl Acad Sci USA,1997,94(4):1298-1303.
20.Wallace GR,Verity DH,Delamaine LJ et al.MIC-A allele profiles and HLA class associations in Beh(?)et's disease[J].Immunogenetics,1999,49(7-8):613-617.
21.Goto K,Ota M,Ohno S,et al.MICA gene and ankylosing spondylitis:linkage analysis via a transmembrane encoded triplet repeat polymorphism[J].Tissus Antigens,1997,49(5):503-507.
22.Yabuki K,et al.Triplet repeat polymorphism in the MICA gene in HLA-B27position and negative cancasicm patients with anklosing spondylitis[J].Human Immunology,1999,60(1):83-86.
23.Kawabata Y,lkegami H,Kawaguchi Y,et al.Age-related association of MHC class Ⅰ chain-related gene A(MICA) with type 1(insulin-dependent)diabetes mellitus[J].Hum Immunology,2000,61(6):624-629.
24.Park Y,Lee H,Sanjeevi C B,et al.MICA Polymorphism is association of type 1diabetes in the Korean population[J].Diabetes Care,2001,24(1):33-38.
25.Tica V,Nikitina-Zake L,Donadi E,et al.MIC-A genotypes 4/5.1 and 9/9 are positively associated with type 1 diabetes mellitus in Brazilian population[J].Ann N Y Acad Sci,2003,1005:310-313.
26.Ochard TR,Dhar A,Simmons JD,et al.MHC class Ⅰ chain-like gene A (MICA) and its associations with inflammatory bowel disease and peripheral arthropathy[J].Clin Exp Immunol,2001,126(3):437-440.
27.Lopez-Vazquez A,Rodrigo L,Mina-Blanco A,et al.Extended human leukocyte antigen haplotype EH18.1 influences progression to hepatocellular carcinoma in patients with hepatitis C virus infection[J].Infect Dis,2004,189(6):957-963.
28.Lopez-Vazquez A,Fuentes D,Rodrigo L,et al.MHC class Ⅰ region plays a role in the development of diverse clinical forms of celiac disease in a Saharawi population[J].AmJ Gastroenterol,2004,99(4):662-667.
29.Karacki PS,Gao X,Thio CL,et al.MICA and recovery from hepatitis C virus and hepatitis B virus infections[J].Genes and Immunity,2004,5(4):261-266.
30.Liu CJ,Lee YJ,Chang KW,et al.Polymorphism of the MICA gene and risk for oral submucous fibrosis[J].J Oral Pathol Med,2004,33(1):1-6.
31.班贵宏,褚嘉佑,冒长峙,等.MICA基因与系统性红斑狼疮关系的研究[J].中华医学遗传学杂志,2002,19(4):298-301.
32.田伟,罗奇志,李立新,等.湖南地区鼻咽癌MHC-I类链相关基因A第5外显子微卫星多态性研究[J].中华医学遗传学杂志,2005,22(3):309-312.
33.黄柏胜,罗奇志,李立新,等.湖南汉族肾移植受者MICA*008/5.1基因与HCMV感染的关系[J].中南大学学报(医学版),2006,31(4):479-482.
34.李立新,田伟,罗奇志,等.湖南地区白血病MHC-Ⅰ类链相关基因A微卫星多态性分析[J].中国免疫学杂志,2007,23(2):129-133.
35.McDermott A.B,Cohen S.B,Zuckerman J.N,et al.Human leucocyte antigens influence the immune response to a pre-s/s hepatitis B Vaccine.Vaccine,1999,17:330-339.
36.Desombere I,Willems A,Leroux - Roels G,et al.Response to hepatitis B vaccine:multiple HLA genes are involved[J].Tissue Antigens,1998,51(6):593-604.
37.刘蓬勃,徐慧文,王学良,等.乙肝疫苗接种无、弱应答与遗传因素关系[J].第 四军医大学学报,2000;21(1):30-33.
38.Thio CL,Carrington M,Marti D,et al.Class Ⅱ HLA alleles and hepatitis B virus persistence in African Americans[J].J Infect Dis,1999,179(4):1004-1006.
39.蒋业贵,王宇明.乙型肝炎患者人类白细胞抗原-DRB1、-DQA1、-DQB1等位基因多态性分析[J].中华流行病学杂志,2004,25(4):337-340.
40.Han Y.-N.,Yang J.-L.,Zhen S.-G.,Tang Q.and Zhu W.Relationship of human leukocyte antigen class Ⅱ genes with the susceptibility hepatitis to B virus infection and the response to interferon in HBV-infected patients[J].World Journal of Gastroenterology,2005,11(36):5721-5724.
41.邓小梅,王金凤,邹雄,等.乙型肝炎病毒感染与宿主淋巴细胞HLA-Ⅰ类分子关系的研究[J].山东大学学报(医学版),2006,12(44):1258-1264.
42.Vivier E,Tomasello E and Paul P.Lymphocyte activation via NKG2D:towards a new paradigm in immune recognition[J]Curt Opin in Immunol.2002,14(3):306-311.
43.陈敏,彭明利,石庆凤,等.乙型肝炎病毒全基因转染L02细胞模型的建立及其HLA-A,B,C和MHCI类链相关基因A/B分子的表达[J].中华肝脏病杂志,2007,15(6):417-421.
44.Yongyan Chen,Min Cheng,Zhigang Tian.Hepatitis B Virus Down-Regulates Expressions of MHC Class Ⅰ Molecules on Hepatoplastoma Cell Line[J].Cellular Molecular Immunology,2006,5(3):373-378.
45.Tang KF,Chen M,Xie J,et al.Inhibition of hepatitis B vires replication by small Interference RNA induces expression of MICA in HepG2.2.15 cells[J].Med Microbiol Immunol,2009,198(1):27-32.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |