铅胁迫对中华常春藤生长和生理特性的影响
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摘要
【目的】通过不同Pb2+对中华常春藤进行胁迫处理,探讨不同浓度Pb2+胁迫对植株的生长性状和生理特性的影响,为中华常春藤的栽培推广以及选择铅高富集植物提供一定理论依据。【方法】以当年生中华常春藤扦插苗为研究对象,对其进行不同浓度Pb(CH3COO)2溶液胁迫处理,并测定各项生长及生理指标。【结果】低浓度铅胁迫下(50、200μmol/L),中华常春藤株高、节间距及茎、叶生物量以及总生物量都显著降低,植物体内MDA含量也显著上升,同时,可溶性蛋白含量、抗氧化酶CAT、POD活性亦显著增加;而高浓度铅胁迫下(400、800μmol/L),植株株高、节间距显著增加,但生物量无显著变化,植株茎段呈现明显徒长现象。同时,植株体内的氧自由基含量、可溶性蛋白含量及抗氧化酶SOD活性亦显著增加。【结论】中华常春藤对铅胁迫具有较强的耐性,可通过激活自身的抗氧化系统及增加可溶性蛋白来抵御铅胁迫下活性氧对其的伤害;此外,低浓度的铅胁迫可使其生长受到抑制,而较高浓度的铅胁迫却会刺激该植物的徒长。
[purpose]In order to investigate the effect of lead stress on the seedling growth characteristics of Hedera nepalensis var. sinensis, and the effects on growth and physiological characteristics were studied under lead stress to provide a theoretical base for lead resistant cultivation and the selection of lead-tolerant plants.[Method]H. nepalensis var. sinensis were used as the experiment materials, the growth and physiological characteristics were determined under different concentrations of Pb(CH3 COO)2 treatment groups.[Result]The plant height, internodes' spacing, stem biomass, leaf biomass and total biomass were significantly decreased under the lower Pb2+ concentration stresses(50, 200 μmol/L), and the MDA content, the soluble protein content, the antioxidant enzyme CAT and POD activities in plant leaves were also increased. While under the higher Pb2+ concentration stresses(400, 800 μmol/L), the plant height and the internodes' spacing were significantly increased, but the biomass did not changed significantly, the stems appear to be significantly longer; at the same time, the soluble protein content, oxygen free radical content and antioxidant enzyme SOD activity also increased significantly.[Conclusion]This study shows that H. nepalensis var. sinensis has strong tolerance to lead stress and could resist the damage of reactive oxygen under lead stress by activating its antioxidant system and increasing the soluble protein. In addition, the plants will be inhibited to growth by the lower concentration lead stress while be stimulated to excessive growth by the higher concentration lead stress.
[purpose]In order to investigate the effect of lead stress on the seedling growth characteristics of Hedera nepalensis var. sinensis, and the effects on growth and physiological characteristics were studied under lead stress to provide a theoretical base for lead resistant cultivation and the selection of lead-tolerant plants.[Method]H. nepalensis var. sinensis were used as the experiment materials, the growth and physiological characteristics were determined under different concentrations of Pb(CH3 COO)2 treatment groups.[Result]The plant height, internodes' spacing, stem biomass, leaf biomass and total biomass were significantly decreased under the lower Pb2+ concentration stresses(50, 200 μmol/L), and the MDA content, the soluble protein content, the antioxidant enzyme CAT and POD activities in plant leaves were also increased. While under the higher Pb2+ concentration stresses(400, 800 μmol/L), the plant height and the internodes' spacing were significantly increased, but the biomass did not changed significantly, the stems appear to be significantly longer; at the same time, the soluble protein content, oxygen free radical content and antioxidant enzyme SOD activity also increased significantly.[Conclusion]This study shows that H. nepalensis var. sinensis has strong tolerance to lead stress and could resist the damage of reactive oxygen under lead stress by activating its antioxidant system and increasing the soluble protein. In addition, the plants will be inhibited to growth by the lower concentration lead stress while be stimulated to excessive growth by the higher concentration lead stress.
引文
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[19]王艳,王进鑫,马惠芳,等.干旱和铅胁迫对刺槐和紫穗槐抗氧化酶活性的影响[J].西北林学院学报,2011,26(6):24.
[20]薛亮,刘建锋,史胜青,等.植物响应重金属胁迫的蛋白质组学研究进展[J].草业学报,2013,22(4):300.
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[23]陶玲,郭永春,李萍,等.铅胁迫对3种豆类作物生理特性的影响[J].中国农学通报,2012,28(33):78.
[24]周朝彬,胡庭兴,胥晓刚,等.铅胁迫对草木樨抗氧化系统的影响[J].草业科学,2006(3):43.
[25]焦树英,徐家林,李红利,等.芒草对NaCl和PEG胁迫的生理响应及相关性分析[J].中国草地学报,2010,32(5):21.
[2]侯晓龙,陈加松,刘爱琴,等.Pb胁迫对金丝草和柳叶箬生长及富Pb特征的影响[J].福建农林大学学报(自然科学版),2012,41(3):286.
[3]ESTRELLA-GóMEZ N,MENDOZA-CóZATL D,MORENO-SáNCHEZ R,et al.The Pb-hyperaccumulator aquatic fern Salvinia minima Baker,responds to Pb2+by increasing phytochelatins via changes in SmPCS expression and in phytochelatin synthase activity[J].Aquatic Toxicology,2009,91(4):320.DOI:10.1016/j.aquatox.2008.11.002.
[4]朱启红,夏红霞.铅胁迫对蜈蚣草抗氧化酶系统和叶绿素含量的影响[J].贵州农业科学,2012,40(4):56.
[5]赵钢,邹亮,彭镰心,等.铅胁迫对苦荞生理特性的影响[J].江苏农业科学,2012,40(7):98.DOI:10.3969/j.issn.1002-1302.2012.07.035.
[6]王慧忠,张新全,何翠屏.Pb对匍匐翦股颖根系超氧化物歧化酶活性的影响[J].农业环境科学学报,2006,25(3):644.DOI:10.3321/j.issn:1672-2043.2006.03.020.
[7]李秀芬,张德顺,王小青,等.常春藤应用概述[J].山东林业科技,2002(3):39.
[8]陈光芹,周银丽,杨嫦丽,等.不同处理对常春藤枝条倒插成活的影响[J].安徽农业科学,2009,37(28):3579.DOI:10.3969/j.issn.0517-6611.2009.28.055.
[9]杨海东,娄义龙,赵建华.影响常春藤生长的因素分析[J].贵州农业科学,2004(5):20.
[10]张存旭,杨锋利,袁秀平.常春藤离体快繁技术[J].浙江林学院学报,2005,22(2):241.DOI:10.3969/j.issn.2095-0756.2005.02.025.
[11]沈漫.常春藤质膜透性和内源激素与抗寒性关系初探[J].园艺学报,2005(1):141.
[12]张朝阳,许桂芳,向左湘.干旱胁迫对4种常绿藤本植物抗性生理生化指标的影响[J].江西农业学报,2008,20(12):42.
[13]夏江宝,张光灿,许景伟,等.干旱胁迫下常春藤净光合速率日变化及其影响因子分析[J].水土保持通报,2010,30(3):78.
[14]赵和文,崔金腾,王杰,等.干旱胁迫下常春藤响应的生理生化机制[J].中国农学通报,2013,29(7):12.DOI:10.3969/j.issn.1000-6850.2013.07.003.
[15]张雪平,王雪娟,张金云,等.干旱胁迫及复水对银边常春藤生长和生理指标的影响[J].浙江农业学报,2015,27(2):194.
[16]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:111.
[17]夏建国,兰海霞,吴德勇.铅胁迫对茶树生长及叶片生理指标的影响[J].农业环境科学学报,2010,29(1):43.
[18]孟晓霞,龙巍,郑超,等.铅胁迫对西南山梗菜生长及逆境生理指标的影响[J].四川农业大学学报,2012,30(3):336.
[19]王艳,王进鑫,马惠芳,等.干旱和铅胁迫对刺槐和紫穗槐抗氧化酶活性的影响[J].西北林学院学报,2011,26(6):24.
[20]薛亮,刘建锋,史胜青,等.植物响应重金属胁迫的蛋白质组学研究进展[J].草业学报,2013,22(4):300.
[21]NEHNEVAJOVA E,LYUBENOVA L,HERZIG R,et al.Metal accumulation and response of antioxidant enzymes in seedlings and adult sunflower mutants with improved metal removal traits on a metal-contaminated soil[J].Environmental and Experimental Botany,2012,76(2):39.
[22]ANJUM N A,AHMAD I,MOHMOOD I,et al.Modulation of glutathione and its related enzymes in plants’responses to toxic metals and metalloids-A review[J].Environmental and Experimental Botany,2012,75(1):307.DOI:10.1016/j.envexpbot.2011.07.002..
[23]陶玲,郭永春,李萍,等.铅胁迫对3种豆类作物生理特性的影响[J].中国农学通报,2012,28(33):78.
[24]周朝彬,胡庭兴,胥晓刚,等.铅胁迫对草木樨抗氧化系统的影响[J].草业科学,2006(3):43.
[25]焦树英,徐家林,李红利,等.芒草对NaCl和PEG胁迫的生理响应及相关性分析[J].中国草地学报,2010,32(5):21.