间充质干细胞在骨髓造血微环境对造血的调控作用及其研究进展
摘要
<正>骨髓间充质干细胞(bone mesenchymal stemcells,BMSCs)是骨髓内多潜能基质细胞,可以分化为成骨、软骨、脂肪、成肌和神经胶质等多种成体细胞。造血干细胞(hematopoietic stem cell,HSC)是血液系统中的成体干细胞,具有长期自我更新的能力以及分化成各类成熟血细胞的能力。1978年Scho-
引文
[1]Schofield R.The relationship between the spleen colonyforming cell and the haemopoietic stem cell[J].Blood Cells,1978,4(2):7-25.
[2]Dexter T M,Allen T D,Lajtha L G.Conditions controlling the proliferation of haemopoietic stem cells in vitro[J].J Cell Physiol,1977,91:335-344.
[3]Taichman R S,Emerson S G.Human osteoblasts support hematopoiesis through the production of granulocyte colonystimulating factor[J].J Exp Med,1994,179:1677-1682.
[4]肖燕妮，张曦，李忠俊.脐血间充质干细胞在造血干细胞移植中的临床应用[J].中国输血杂志，2013,26(4):315-318.
[5]王健民，宋宁霞.间充质干细胞与造血干细胞移植相关研究的进展[J].内科理论与实践，2010,5(1):24-29.
[6]汪姝玥，林凡莉，钱怡，等.不同共培养模式下间充质干细胞对造血干细胞增殖的作用[J].中国组织工程研究，2018,22(13):2068-2074.
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[9]Li X,Zhang Y,Qi G.Evaluation of isolation methods and culture conditions for rat bone marrow mesenchymal stem cells[J].Cytotechnology,2013,65(3):323-334.
[10]Encina N R,Billotte W G,Hofmann M C.Immunomagnetic isolation of osteoprogenitors from human bone marrow stroma[J].Lab Invest,1999,79(4):449-457.
[11]Lisignoli G,Remiddi G,Cattini L,et al.An elevated number of differentiated osteoblast colonies can be obtained from rat bone marrow stromal cells using a gradient isolation procedure[J].Connect Tissue Res,2001,42(1):49-58.
[12]Zhang W,Zhang F,Shi H,et al.Comparisons of rabbit bone marrow mesenchymal stem cell isolation and culture methods in vitro[J].PLoS One,2014,9(2):e88794.
[13]李晓峰，赵劲民，苏伟，等.大鼠骨髓间充质干细胞的培养与鉴定[J].中国组织工程研究与临床康复，2011,15(10):1721-1725.
[14]张颖，周倍伊.复方扶芳藤合剂对大鼠骨髓间充质干细胞Notch信号通路的调节作用[J].中国实验方剂学，2013,19(18):206-209.
[15]Khodadi E,Asnafi A A,Shahrabi S,et al.Bone marrow niche in immune thrombocytopenia:a focus on megakaryopoiesis[J].Ann Hematol,2016,95(11):1765-1776.
[16]Calvi L M,Adams G B,Weibrecht K W,et al.Osteoblastic cells regulate the haematopoietic stem cell niche[J].Nature,2003,425(6960):841-846.
[17]Zhang J,Niu C,Ye L,et al.Identification of the haematopoietic stem cell niche and control of the niche size[J].Nature,2003,425:836-841.
[18]Ding L,Saunders T L.Endothelial and perivascular cells maintainhaematopoietic stem cells[J].Nature,2012,481(7382):457-462.
[19]Winkler I G,Barbier V,Nowlan B,et al.Vascular niche E-selectin regulates hematopoietic stem cell dormancy,self renewal and chemoresistance[J].Nat Med,2012,18(11):1651-1657.
[20]Heissig B,Hattori K,Dias S,et al.Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand[J].Cell,2002,109:625-637.
[21]Méndez-Ferrer S,Michurina T V,Ferraro F,et al.Mesenchymal and haematopoietic stem cells form a unique bone marrow niche[J].Nature,2010,466(9262):829-834.
[22]Ding L,Zhu H,Yang Y,et al.Functional mesenchymalstem cells remain present in the bone marrow microenvironment of leukemia patients post allogeneic hematopoietic stem cell transplantation[J].Leuk Lymphoma,2014,55(7):1635-1644.
[23]Kim E J,Kim N,Cho S G.The potential use of mesenchymal stem cells in hematopoietic stem cell transplantation[J].Exp Mol Med,2013,45(1):2-4.
[24]徐志娟，盛立霞，楼燕茹，等.脐带血来源的间充质干细胞对CD34+细胞增殖的支持作用及其机制[J].中国实验血液学杂志，2015,23(3):802-808.
[25]杜幸军，余海彬，刘玉峰，等.胎盘间充质干细胞联合外周血造血干细胞移植对严重联合免疫缺陷小鼠造血的影响[J].中华实验外科杂志，2016,33(3):753-756.
[26]王金福，王立娟，Jenny Harrintong，等.骨髓间充质干细胞与造血干细胞共培养对脐血造血干细胞扩增的作用[J].浙江大学学报，2003,30(1):93-96.
[27]Bianco P,Robey P G,Simmons P J.Mesenchymalstemcells:revisiting history,concepts,and assays[J].Cell Stem Cell,2008,2(4):313-319.
[28]Bernardo M E,Cometa A M,Locatelli F.Mesenchymal stromalcells:a novel and effective strategy for facilitating engraftmentand accelerating hematopoietic recovery after transplantation[J].Bone Marrow Transplant,2012,47(3):323-329．
[29]Asada N,Kunisaki Y,Pierce H,et al.Differential cytokine contributions of perivascular haematopoietic stem cell niches[J].Nat Cell Biol,2017,19:214-223.
[30]Walenda T,Bokermann G,Ventura-Ferreira M S et al.Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem andprogenitor cells[J].Exp Hematol,2011,39(6):617-628．
[31]Stier S,Cheng T,Dombkowski D,et al.Notch1activation increases hematopoietic stem cell self-renewal in vivo and favours lymphoid over myeloid lineage outcome[J].Blood,2002,99(7):2.
[32]Artavanis T S,Artavanis-Tsakonas S,Rand M D,et al.Notch signaling:cell fate control and signal integration in development[J].Science,1999,284(5415):770-776.
[33]Sugiyama T,Kohara H,Noda M,et al.Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bonemarrow stromal cell niches[J].Immunity,2006,25:977-988.
[34]Sang C,Zhang Y,Chen F,et al.Tumor necrosis factor alpha suppresses osteogenic differentiation of MSCs by inhibiting semaphorin 3B via Wnt/β-catenin signaling in estrogendeficiency induced osteoporosis[J].Bone,2016(84):78-87.
[35]Clevers H,Loh K M,Nusse R.An integralprogram for tissue renewal and regeneration:Wnt signaling andstem cell control[J].Science,2014,346(6205):1248012．
[36]Maedonaid P R,Progias P,Ciani B,et al.Structure of theextracellular domain of tie receptor tyrosine kinases and localization of the angiopoietin-binding epitope[J].J Biol Chem,2006,281(38):28408-28414．
[37]Puri M C,Bernstein A.Requirement for the Tie family of receptor tyrosine kinases in adult but not fetal hematopoiesis[J].Proc Natl Acad Sci USA,2003,100(22):12753-12758.
[38]Bigas A,Guiu J,Norton L.Notch and Wnt signaling in theemergence of hematopoiefie stem cells[J].Blood Cells Mol Dis,2013,51(4):264-270．
[39]张雨，周倍伊.复方扶芳藤合剂对小鼠外周血干细胞动员作用的实验研究[J].中国实验方剂学，2012,18(7):212-215.
[40]张颖，周倍伊.复方扶芳藤合剂对大鼠骨髓间充质干细胞Notch信号通路的调节作用[J].中国实验方剂学，2013,19(18):206-209.
[2]Dexter T M,Allen T D,Lajtha L G.Conditions controlling the proliferation of haemopoietic stem cells in vitro[J].J Cell Physiol,1977,91:335-344.
[3]Taichman R S,Emerson S G.Human osteoblasts support hematopoiesis through the production of granulocyte colonystimulating factor[J].J Exp Med,1994,179:1677-1682.
[4]肖燕妮，张曦，李忠俊.脐血间充质干细胞在造血干细胞移植中的临床应用[J].中国输血杂志，2013,26(4):315-318.
[5]王健民，宋宁霞.间充质干细胞与造血干细胞移植相关研究的进展[J].内科理论与实践，2010,5(1):24-29.
[6]汪姝玥，林凡莉，钱怡，等.不同共培养模式下间充质干细胞对造血干细胞增殖的作用[J].中国组织工程研究，2018,22(13):2068-2074.
[7]Polisetti N,Chaitanya V G,Babu P P,et al.Isolationcharacterization and differentiation potential of rat bone marrow stromal cells[J].Neurol India,2010,58(2):201-208.
[8]Tondreau T,Lagneaux L,Dejeneffe M,et al.Isolation of mesenchymal stem cells by plastic adhesion or negative selection:phenotype,proliferation kinetics and differentiation potential[J].Cytotherapy,2004,6(4):372-379.
[9]Li X,Zhang Y,Qi G.Evaluation of isolation methods and culture conditions for rat bone marrow mesenchymal stem cells[J].Cytotechnology,2013,65(3):323-334.
[10]Encina N R,Billotte W G,Hofmann M C.Immunomagnetic isolation of osteoprogenitors from human bone marrow stroma[J].Lab Invest,1999,79(4):449-457.
[11]Lisignoli G,Remiddi G,Cattini L,et al.An elevated number of differentiated osteoblast colonies can be obtained from rat bone marrow stromal cells using a gradient isolation procedure[J].Connect Tissue Res,2001,42(1):49-58.
[12]Zhang W,Zhang F,Shi H,et al.Comparisons of rabbit bone marrow mesenchymal stem cell isolation and culture methods in vitro[J].PLoS One,2014,9(2):e88794.
[13]李晓峰，赵劲民，苏伟，等.大鼠骨髓间充质干细胞的培养与鉴定[J].中国组织工程研究与临床康复，2011,15(10):1721-1725.
[14]张颖，周倍伊.复方扶芳藤合剂对大鼠骨髓间充质干细胞Notch信号通路的调节作用[J].中国实验方剂学，2013,19(18):206-209.
[15]Khodadi E,Asnafi A A,Shahrabi S,et al.Bone marrow niche in immune thrombocytopenia:a focus on megakaryopoiesis[J].Ann Hematol,2016,95(11):1765-1776.
[16]Calvi L M,Adams G B,Weibrecht K W,et al.Osteoblastic cells regulate the haematopoietic stem cell niche[J].Nature,2003,425(6960):841-846.
[17]Zhang J,Niu C,Ye L,et al.Identification of the haematopoietic stem cell niche and control of the niche size[J].Nature,2003,425:836-841.
[18]Ding L,Saunders T L.Endothelial and perivascular cells maintainhaematopoietic stem cells[J].Nature,2012,481(7382):457-462.
[19]Winkler I G,Barbier V,Nowlan B,et al.Vascular niche E-selectin regulates hematopoietic stem cell dormancy,self renewal and chemoresistance[J].Nat Med,2012,18(11):1651-1657.
[20]Heissig B,Hattori K,Dias S,et al.Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand[J].Cell,2002,109:625-637.
[21]Méndez-Ferrer S,Michurina T V,Ferraro F,et al.Mesenchymal and haematopoietic stem cells form a unique bone marrow niche[J].Nature,2010,466(9262):829-834.
[22]Ding L,Zhu H,Yang Y,et al.Functional mesenchymalstem cells remain present in the bone marrow microenvironment of leukemia patients post allogeneic hematopoietic stem cell transplantation[J].Leuk Lymphoma,2014,55(7):1635-1644.
[23]Kim E J,Kim N,Cho S G.The potential use of mesenchymal stem cells in hematopoietic stem cell transplantation[J].Exp Mol Med,2013,45(1):2-4.
[24]徐志娟，盛立霞，楼燕茹，等.脐带血来源的间充质干细胞对CD34+细胞增殖的支持作用及其机制[J].中国实验血液学杂志，2015,23(3):802-808.
[25]杜幸军，余海彬，刘玉峰，等.胎盘间充质干细胞联合外周血造血干细胞移植对严重联合免疫缺陷小鼠造血的影响[J].中华实验外科杂志，2016,33(3):753-756.
[26]王金福，王立娟，Jenny Harrintong，等.骨髓间充质干细胞与造血干细胞共培养对脐血造血干细胞扩增的作用[J].浙江大学学报，2003,30(1):93-96.
[27]Bianco P,Robey P G,Simmons P J.Mesenchymalstemcells:revisiting history,concepts,and assays[J].Cell Stem Cell,2008,2(4):313-319.
[28]Bernardo M E,Cometa A M,Locatelli F.Mesenchymal stromalcells:a novel and effective strategy for facilitating engraftmentand accelerating hematopoietic recovery after transplantation[J].Bone Marrow Transplant,2012,47(3):323-329．
[29]Asada N,Kunisaki Y,Pierce H,et al.Differential cytokine contributions of perivascular haematopoietic stem cell niches[J].Nat Cell Biol,2017,19:214-223.
[30]Walenda T,Bokermann G,Ventura-Ferreira M S et al.Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem andprogenitor cells[J].Exp Hematol,2011,39(6):617-628．
[31]Stier S,Cheng T,Dombkowski D,et al.Notch1activation increases hematopoietic stem cell self-renewal in vivo and favours lymphoid over myeloid lineage outcome[J].Blood,2002,99(7):2.
[32]Artavanis T S,Artavanis-Tsakonas S,Rand M D,et al.Notch signaling:cell fate control and signal integration in development[J].Science,1999,284(5415):770-776.
[33]Sugiyama T,Kohara H,Noda M,et al.Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bonemarrow stromal cell niches[J].Immunity,2006,25:977-988.
[34]Sang C,Zhang Y,Chen F,et al.Tumor necrosis factor alpha suppresses osteogenic differentiation of MSCs by inhibiting semaphorin 3B via Wnt/β-catenin signaling in estrogendeficiency induced osteoporosis[J].Bone,2016(84):78-87.
[35]Clevers H,Loh K M,Nusse R.An integralprogram for tissue renewal and regeneration:Wnt signaling andstem cell control[J].Science,2014,346(6205):1248012．
[36]Maedonaid P R,Progias P,Ciani B,et al.Structure of theextracellular domain of tie receptor tyrosine kinases and localization of the angiopoietin-binding epitope[J].J Biol Chem,2006,281(38):28408-28414．
[37]Puri M C,Bernstein A.Requirement for the Tie family of receptor tyrosine kinases in adult but not fetal hematopoiesis[J].Proc Natl Acad Sci USA,2003,100(22):12753-12758.
[38]Bigas A,Guiu J,Norton L.Notch and Wnt signaling in theemergence of hematopoiefie stem cells[J].Blood Cells Mol Dis,2013,51(4):264-270．
[39]张雨，周倍伊.复方扶芳藤合剂对小鼠外周血干细胞动员作用的实验研究[J].中国实验方剂学，2012,18(7):212-215.
[40]张颖，周倍伊.复方扶芳藤合剂对大鼠骨髓间充质干细胞Notch信号通路的调节作用[J].中国实验方剂学，2013,19(18):206-209.