决明子多糖含量测定方法
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摘要
本研究目的在于寻找最适合测定决明子多糖含量的方法,通过气相色谱法分析决明子多糖的单糖组成,根据其单糖摩尔比通过标准单糖来模拟决明子多糖,其测定结果与葡萄糖或甘露糖为标准单糖测定结果进行换算,以便更加准确测定决明子多糖的含量。从混标的线性关系看,苯酚硫酸法的相关系数比蒽酮硫酸法高(0.999 4 vs 0.996 8)。不同单糖标准的苯酚硫酸法测定决明子多糖的校正系数相对差异在合理范围,测得的结果也符合决明子多糖中甘露糖、混标、葡萄糖含量顺序,因此苯酚硫酸法更适合用来测定决明子多糖的含量。
In this paper,the aim is to find the best method for determination of polysaccharide in Semen Cassiae.The monosaccharide composition of semen cassiae polysaccharide was analyzed by gas chromatography.According to the molar ratio of monosaccharide,the polysaccharide of Semen Cassiae was simulated by standard monosaccharide.The results were converted to glucose or mannose as standard monosaccharide in order to determine the content of polysaccharide of Semen Cassiae more accurately.From the linearity of the mixed standards,the correlation coefficient of Phenol-sulfuric acid method is higher than Anthrone-sulfuric acid method(0.999 4 vs 0.996 8).The relative difference of correction coefficient of polysaccharide in Semen Cassiae by different monosaccharide standard of phenol-sulfuric acid method is in the reasonable range.The result is also conform to the content of the order in Semen Cassiae that the highest contents of the mannose and the lowest contents of the glucose.Therefore,Phenol-sulfuric acid is more suitable for the determination of the contents of polysaccharide in Semen Cassiae.
In this paper,the aim is to find the best method for determination of polysaccharide in Semen Cassiae.The monosaccharide composition of semen cassiae polysaccharide was analyzed by gas chromatography.According to the molar ratio of monosaccharide,the polysaccharide of Semen Cassiae was simulated by standard monosaccharide.The results were converted to glucose or mannose as standard monosaccharide in order to determine the content of polysaccharide of Semen Cassiae more accurately.From the linearity of the mixed standards,the correlation coefficient of Phenol-sulfuric acid method is higher than Anthrone-sulfuric acid method(0.999 4 vs 0.996 8).The relative difference of correction coefficient of polysaccharide in Semen Cassiae by different monosaccharide standard of phenol-sulfuric acid method is in the reasonable range.The result is also conform to the content of the order in Semen Cassiae that the highest contents of the mannose and the lowest contents of the glucose.Therefore,Phenol-sulfuric acid is more suitable for the determination of the contents of polysaccharide in Semen Cassiae.
引文
[1] 徐义龙.决明子化学成分及炮制对其影响研究[D].北京:中国中医科学院,2014.
[2] 张毅,黄小平,翁代群,等.HPLC测定不同产地决明子中蒽醌类成分[J].中国中药杂志,2008,33(23):2797-2799.
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[4] 邓泽元,刘娟,余迎利,等.决明子水溶性多糖提取的研究[J].食品科学,2002:72-75
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[8] 刘淑敏,孙超,谢伟华.决明子提取物对高血脂模型小鼠聚脂基因表达的影响[J].中草药,2009:583-587.
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[11] 奚光兴.苦瓜藤多糖的分离纯化及理化性质和单糖组成研究[D].南昌:南昌大学,2013.
[12] CHEN Z,ZHANG W,TANG X Y,et al.Extraction and characterization of polysaccharides from Semen Cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC[J].Carbohydrate Polymers,2016,144(25):263-270.
[13] 吕明霞,李媛,张飞,等.气相色谱法分析北方水果中膳食纤维的单糖组成[J].中国食品学报,2012,12(2):213-218.
[14] 张琳,邓贵华.蛹虫草子实体中多糖含量不同测定方法的比较[J].中药材,2012,35(5):733-736.
[15] 孟玲,王兰英,梁大勇,等.桦褐孔菌多糖测定方法的比较[J].食品研究与开发,2010,31(4):108-110.
[16] FENG L,YIN J Y,NIE S P,et al.Fractionation,physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia [J].International Journal of Biological Macromolecules,2016:91:946-953
[17] 王莹,许玮仪,李丽潇,等.注射用黄芪多糖相对分子质量测定方法的比较及研究[J].药学学报,2019,2:348-353.
[18] 范嘉龙,马丽苹,叶红,等.苦丁冬青苦丁茶多糖的单糖组成分析[J].食品科学,2013,34(11):72-76.
[19] 石浩,王仁才,庞立,等.糖枣多糖的单糖组成分析[J].食品科学,2015,36(6):169-172.
[20] 钟方晓,任海华,李岩.多糖含量测定方法比较[J].时珍国医国药,2007,18(8):1916-1917.
[2] 张毅,黄小平,翁代群,等.HPLC测定不同产地决明子中蒽醌类成分[J].中国中药杂志,2008,33(23):2797-2799.
[3] 骆宜,刘斌.HPLC法测定决明子中3个萘并吡喃酮类成分的含量[J].中华中医药杂志,2016,31(9):3774-3777.
[4] 邓泽元,刘娟,余迎利,等.决明子水溶性多糖提取的研究[J].食品科学,2002:72-75
[5] 吕浩然.决明子调脂减肥的活性成分研究[D].广州:华南理工大学,2012.
[6] 刘菊秀,苗戎,狄俊英,郝志强.决明子降压作用的实验研究[J].天津中医,1990:37-38.
[7] 毛万姮,商黔惠,刘爱东,等.决明子提取物对大鼠主动脉扩血管作用的机制[J].中华高血压杂志,2010:60-64.
[8] 刘淑敏,孙超,谢伟华.决明子提取物对高血脂模型小鼠聚脂基因表达的影响[J].中草药,2009:583-587.
[9] 林卓慧,熊颖.决明子提取物对高血脂大鼠模型血脂的影响[J].新中医,2010:111-112.
[10] 韩昌志.决明子的明目作用[J].中国医院药学杂志,1993:10-11.
[11] 奚光兴.苦瓜藤多糖的分离纯化及理化性质和单糖组成研究[D].南昌:南昌大学,2013.
[12] CHEN Z,ZHANG W,TANG X Y,et al.Extraction and characterization of polysaccharides from Semen Cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC[J].Carbohydrate Polymers,2016,144(25):263-270.
[13] 吕明霞,李媛,张飞,等.气相色谱法分析北方水果中膳食纤维的单糖组成[J].中国食品学报,2012,12(2):213-218.
[14] 张琳,邓贵华.蛹虫草子实体中多糖含量不同测定方法的比较[J].中药材,2012,35(5):733-736.
[15] 孟玲,王兰英,梁大勇,等.桦褐孔菌多糖测定方法的比较[J].食品研究与开发,2010,31(4):108-110.
[16] FENG L,YIN J Y,NIE S P,et al.Fractionation,physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia [J].International Journal of Biological Macromolecules,2016:91:946-953
[17] 王莹,许玮仪,李丽潇,等.注射用黄芪多糖相对分子质量测定方法的比较及研究[J].药学学报,2019,2:348-353.
[18] 范嘉龙,马丽苹,叶红,等.苦丁冬青苦丁茶多糖的单糖组成分析[J].食品科学,2013,34(11):72-76.
[19] 石浩,王仁才,庞立,等.糖枣多糖的单糖组成分析[J].食品科学,2015,36(6):169-172.
[20] 钟方晓,任海华,李岩.多糖含量测定方法比较[J].时珍国医国药,2007,18(8):1916-1917.