大球盖菇液体菌种培养基碳氮源及培养条件的优化

详细信息    查看全文 | 推荐本文 |

英文篇名：Optimization of Carbon and Nitrogen Sources and Culture Conditions of Liquid Culture Medium of Stropharia rugosoannulata 作者：刘金力 ; 唐琳 ; 陈思 ; 董亚萍 ; 王楠楠 ; 王伟 ; 王彦杰 英文作者：LIU Jin-li;TANG Lin;CHEN Si;DONG Ya-ping;WANG Nan-nan;WANG Wei;WANG Yan-jie;Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-waste in Cold Region,College of Life Science and Technology,Heilongjiang Bayi Agricultural University; 关键词：大球盖菇 ; 液体培养基 ; 碳氮源优化 ; 培养基组分 ; 菌丝体生物量 英文关键词：Stropharia rugosoannulata;;liquid medium;;medium optimizing;;mycelium biomass 中文刊名：黑龙江农业科学 英文刊名：Heilongjiang Agricultural Sciences 机构：黑龙江八一农垦大学生命科学技术学院/寒区环境微生物与农业废弃物资源化利用重点实验室; 出版日期：2019-04-10 出版单位：黑龙江农业科学 年：2019 期：04 基金：黑龙江八一农垦大学大学生创新创业训练计划项目 语种：中文; 页：100-105 页数：6 CN：23-1204/S ISSN：1002-2767 分类号：S646

摘要

为了促进大球盖菇液体菌种扩繁,采用单因素方差分析和正交试验的方法,对菌体培养的碳源、氮源和培养条件进行优化,通过测定大球盖菇菌丝球生物量指标,研究不同碳氮源和不同培养条件对大球盖菇生长的影响。结果表明:大球盖菇最适培养基为葡萄糖20 g·L~(-1),玉米粉10 g·L~(-1),麸皮5 g·L~(-1),KH_2PO_(4 )3 g·L~(-1),MgSO_(4 )0.2 g·L~(-1)。优化后,大球盖菇菌丝体生物量增加了174%,最佳培养条件是pH6,转速120 r·min~(-1)及培养温度25℃。培养基和培养条件的优化可以显著促进大球盖菇菌丝的生长。
        In order to promote the expansion and propagation of the liquid strains of Stropharia rugosoannulata,this paper optimized carbon source,nitrogen source and culture conditions with the one-way analysis of variance and orthogonal experimental design,through the determination of Stropharia rugosoannulata mycelia biomass index,researched effect of the different carbon source,nitrogen source and different culture conditions on Stropharia rugosoannulata mycelial growth.The results showed that the optimal medium was 20 g·L~(-1) glucose,10 g·L-1 maize flour,5 g·L~(-1) bran,3 g·L~(-1) KH_2PO_4 and MgSO40.2 g·L~(-1).After optimization,mycelium biomass increased by 174%,the best culture conditions were pH6,rotating speed 120 r·min~(-1) and the temperature at 25℃.The optimization of culture medium and culture conditions could significantly promote mycelial growth of the Stropharia rugosoannulata.

引文

[1] He P X,Geng L J,Wang J Z, et al.Production, purfication, molecular characterization and bioactivities of exopolysaccharides produced by the wine cap culinary-medicinal mushroom,Stropharia rugosoannulata 2(higher Basidiomycetes)[J].International Journal of Medicinal Mushrooms,2012,14(4): 365-376.
    [2] Wu J,Tokuyama S,Nagai K,et al.Strophasterols A to D with an unprecedented steroid skeleton:From the mushroom Stropharia rugosoannulata[J].Natural Products,2012,124:1-4.
    [3] 郑文彪,吕军美,潘永柱,等.大球盖菇栽培模式比较试验[J].食用菌,2015(2):46-48.
    [4] Zhang W W,Tian G T,Geng X R,et al.Isolation and characterization of a novel lectin from the edible mushroom Stropharia rugosoannulata[J].Molecules,2014,19:19880-19891.
    [5] 周祖法,闫静,王伟科.不同培养料配方栽培大球盖菇试验[J].浙江农业科学,2013(2):149-150.
    [6] 孙兴荣,韩勇武,卞景阳.大球盖菇菌丝体液体发酵培养条件的研究[J].黑龙江农业科学,2016(8):114-117.
    [7] 黄春燕,万鲁长,张柏松.大球盖菇菌丝生长最佳碳源研究[J].山东农业科学,2012,44(1):75-76.
    [8] 黄春燕,万鲁长,张柏松.大球盖菇菌丝生长适宜氮源研究[J].中国食用菌,2012,31(6):18-19,23.
    [9] 余海立,汪瑾雨,毛成凤.正交试验优化大球盖菇富硒发酵工艺[J].食品工业,2018,39(2):103-107.
    [10] 李正鹏,吴萍,陆晓民.大球盖菇液体菌种培养条件的研究[J].中国林副特产,2006(4):83-85.
    [11] 张世敏,和晶亮,邱立友.不同碳氮营养源和培养温度对大球盖菇菌丝生长的影响[J].微生物学杂志,2005,25(6):32-34.
    [12] 萨仁图雅,图力古尔.大球盖菇研究进展[J].食用菌学报,2005,12(4):57-64.
    [13] 孙清波,许广波,梁运江.大球盖菇液体培养基组分的优化试验[J].中国园艺文摘,2011(9):10-12.