超滤-纳滤联用用于干姜中指标性成分6-姜辣素的浓缩
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摘要
目的:探索超滤-纳滤联用技术在干姜中指标性成分6-姜辣素浓缩中的应用。方法:以总蛋白和6-姜辣素透过率为指标,考察超滤过程。在单因素试验的基础上,采用Box-Behnken响应面法,以6-姜辣素的截留率为指标,考察滤膜截留相对分子质量(Mr)、6-姜辣素浓度和p H对纳滤过程的影响。结果:采用截留Mr为10 k Da的滤膜,对总蛋白的去除率为98.89%,6-姜辣素的透过率达到98.55%。纳滤过程最佳工艺参数为:截留Mr为350 Da、6-姜辣素质量浓度为150μg·ml~(-1)、超滤液p H为7.0,对6-姜辣素的实际截留率为95.59%。结论:超滤-纳滤联用技术可有效完成对姜辣素类成分的浓缩,相比传统热浓缩优势明显。
Objective: To apply ultrafiltration-nanofiltration in the concentration of indicator component 6-gingerol in Zingiberis Rhizoma. Methods: The ultrafiltration process was investigated by the permeability rate of total protein and 6-glyphosin. On the basis of single factor test,Box-Behnken response surface method was used to investigate the influences of molecular weight,6-gingerol concentration and p H value on the nanoflitration with the interception rate of 6-gingerol as the indicator. Results: The permeability rate of total protein was 98.89%,and the permeability rate of 6-gingerol was 98.55% by the membrane with interception molecular weight of 10 k Da. The optimal nanofiltration process parameters were as follows: the interception molecular weight was 350 Da,the 6-gingerol concentration was 150 μg·ml~(-1) and the p H value was 7.0,as a result,the actual interception rate of 6-gingerol was 95.59%. Conclusion: Ultrafiltration-nanofiltration coupling technology is more effective for alkaloids concentration than the traditional thermal concentration.
Objective: To apply ultrafiltration-nanofiltration in the concentration of indicator component 6-gingerol in Zingiberis Rhizoma. Methods: The ultrafiltration process was investigated by the permeability rate of total protein and 6-glyphosin. On the basis of single factor test,Box-Behnken response surface method was used to investigate the influences of molecular weight,6-gingerol concentration and p H value on the nanoflitration with the interception rate of 6-gingerol as the indicator. Results: The permeability rate of total protein was 98.89%,and the permeability rate of 6-gingerol was 98.55% by the membrane with interception molecular weight of 10 k Da. The optimal nanofiltration process parameters were as follows: the interception molecular weight was 350 Da,the 6-gingerol concentration was 150 μg·ml~(-1) and the p H value was 7.0,as a result,the actual interception rate of 6-gingerol was 95.59%. Conclusion: Ultrafiltration-nanofiltration coupling technology is more effective for alkaloids concentration than the traditional thermal concentration.
引文
1中国药典[S]. 2015年版.一部. 36-38
2 王哲,金永日,李绪文.干姜药材的化学成分研究[J].中国药房,2017,28(24):3403-3405
3 王志.干姜和中药豨莶草的化学成分研究[D].合肥:安徽大学硕士学位论文,2012
4 李琰,王彦志,李泽之,等.干姜中姜酚类成分的分离鉴定[J].中国药学杂志,2017,37(20):1812-1815
5 芮雯,冯毅凡,吴妍,等.干姜油中姜酚类成分的UPLC/Q-TOF MS分析[J].中草药,2008,39(5):667-668
6 张科卫,马彩霞,缪六舒.干姜、炮姜中成分的比较[J].中成药,2014,36(6):1254-1260
7 孙权,李冰,王星,等.氮酮对6-姜辣素透皮吸收的影响[J].中成药,2018,40(3):583-586
8 刘芸,王珂佳,黄锴,等. HPLC法测定生姜半夏汤单煎与合煎中6 -姜辣素和6-姜醇含量[J].中国生化药物杂志,2015,35(2):168-170
9 洪燕,韩燕全,高家荣,等.干姜中姜酚类成分的提取工艺研究[J].中华中医药学刊,2013,31(10):2100-2103
10 黄鹤归,叶晓川,田连起,等.超临界CO2萃取法与水蒸气蒸馏法提取桂枝-干姜药对的化学成分比较[J].中国实验方剂学杂志,2011,17(24):85-92
11 李存玉,钱祥,杨泽秋,等.基于纳滤技术的消癌平注射液中的钾离子去除工艺优化[J].中成药,2015,37(2):294-297
12 李红宾,石文英,王薇,等.中空纤维复合纳滤膜的研究进展[J].膜科学与技术,2016,36(2):122-131
13 林立刚,叶卉,赵芝莉,等.膜吸附和膜吸收研究进展[J].中国工程科学,2014(12):59-66
14 陈萍,朱胤龙,韦强,等.均匀设计法优选干姜渗滤提取工艺[J].中国实验方剂学杂志,2003,9(3):12-13
15 Yaroshchuk A,Bruening ML,Bernal EEL. Solution-Diffusion-ElectroMigration model and its uses for analysis of nanofiltration,pressureretarded osmosis and forward osmosis in multi-ionic solutions[J]. J Membr Sci,2013,447(22):463-476
16 赵彦彦,袁其朋.有机相纳滤分离过程中浓度、电荷、溶剂对溶质截留行为的影响[J].膜科学与技术,2006,26(5):31-36
17 Fang J,Deng BL. Rejection and modeling of arsenate by nanofiltration:Contributions of convection,diffusion and electromigration to arsenic transport[J]. Membr Sci Technol,2014,453(453):42-51
2 王哲,金永日,李绪文.干姜药材的化学成分研究[J].中国药房,2017,28(24):3403-3405
3 王志.干姜和中药豨莶草的化学成分研究[D].合肥:安徽大学硕士学位论文,2012
4 李琰,王彦志,李泽之,等.干姜中姜酚类成分的分离鉴定[J].中国药学杂志,2017,37(20):1812-1815
5 芮雯,冯毅凡,吴妍,等.干姜油中姜酚类成分的UPLC/Q-TOF MS分析[J].中草药,2008,39(5):667-668
6 张科卫,马彩霞,缪六舒.干姜、炮姜中成分的比较[J].中成药,2014,36(6):1254-1260
7 孙权,李冰,王星,等.氮酮对6-姜辣素透皮吸收的影响[J].中成药,2018,40(3):583-586
8 刘芸,王珂佳,黄锴,等. HPLC法测定生姜半夏汤单煎与合煎中6 -姜辣素和6-姜醇含量[J].中国生化药物杂志,2015,35(2):168-170
9 洪燕,韩燕全,高家荣,等.干姜中姜酚类成分的提取工艺研究[J].中华中医药学刊,2013,31(10):2100-2103
10 黄鹤归,叶晓川,田连起,等.超临界CO2萃取法与水蒸气蒸馏法提取桂枝-干姜药对的化学成分比较[J].中国实验方剂学杂志,2011,17(24):85-92
11 李存玉,钱祥,杨泽秋,等.基于纳滤技术的消癌平注射液中的钾离子去除工艺优化[J].中成药,2015,37(2):294-297
12 李红宾,石文英,王薇,等.中空纤维复合纳滤膜的研究进展[J].膜科学与技术,2016,36(2):122-131
13 林立刚,叶卉,赵芝莉,等.膜吸附和膜吸收研究进展[J].中国工程科学,2014(12):59-66
14 陈萍,朱胤龙,韦强,等.均匀设计法优选干姜渗滤提取工艺[J].中国实验方剂学杂志,2003,9(3):12-13
15 Yaroshchuk A,Bruening ML,Bernal EEL. Solution-Diffusion-ElectroMigration model and its uses for analysis of nanofiltration,pressureretarded osmosis and forward osmosis in multi-ionic solutions[J]. J Membr Sci,2013,447(22):463-476
16 赵彦彦,袁其朋.有机相纳滤分离过程中浓度、电荷、溶剂对溶质截留行为的影响[J].膜科学与技术,2006,26(5):31-36
17 Fang J,Deng BL. Rejection and modeling of arsenate by nanofiltration:Contributions of convection,diffusion and electromigration to arsenic transport[J]. Membr Sci Technol,2014,453(453):42-51