亚硝酸盐型厌氧甲烷氧化过程与应用研究进展
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摘要
亚硝酸盐型厌氧甲烷氧化(nitrite-dependent anaerobic methane oxidation,N-DAMO)是指以亚硝酸盐为电子受体将甲烷厌氧氧化为二氧化碳的微生物生化反应过程。此反应是偶联碳氮循环的关键环节,是微生物碳氮循环的新内容,它的发现对于全球的碳氮物质循环具有重要贡献。基于此,文章概述了N-DAMO过程的生化反应机理;总结了N-DAMO菌富集培养过程中的活性影响因素:NO_2~--N浓度为2.40 mmol?L~(-1)时N-DAMO菌的反硝化活性最大,CH_4分压为49 kPa可基本满足N-DAMO菌对CH_4的需求量,N-DAMO菌最适pH值为7.6左右,最适温度为35℃左右,N-DAMO菌能够适应一定含量的盐度;浅析了N-DAMO过程在海洋、淡水及其湿地等不同生境中的分布特点;在此基础上,探讨了Anammox与N-DAMO的耦合反应机理在污水处理脱氮工艺中的应用,该工艺能够节约大量物质能源、减少经济成本和提高脱氮效率,具有巨大的潜在应用前景。
Nitrite-type anaerobic methane oxidation(N-DAMO)refers to the microbial biochemical process in which methane is anaerobically oxidized to carbon dioxide using nitrite as an electron acceptor.This reaction is the key link of the coupled carbon-nitrogen cycle and the new content of the microbial carbon-nitrogen cycle.Its discovery has an important contribution to the global carbon-nitrogen cycle.Based on this,this paper summarized the biochemical reaction mechanism of N-DAMO process,and summarized the influencing factors of N-DAMO bacterial activity in enrichment and culture process:when the concentration of NO_2~--N was 2.40 mmol?L~(-1),the denitrification activity of N-DAMO bacteria was the highest;the partial pressure of CH_4 was 49KPa,which could basically meet the requirement of N-DAMO bacteria for CH_4,the optimum pH value of N-DAMO bacteria was about 7.6,and the optimum temperature was about 35℃,the N-DAMO bacteria could adapt to a certain content of salinity.The distribution characteristics of N-DAMO process in different habitats such as ocean,freshwater and wetland were analyzed;On this basis,the application of coupling reaction mechanism between Anammox and N-DAMO in wastewater treatment denitrification process was discussed.This process can save a lot of material and energy,reduce economic costs and improve denitrification efficiency,and has great potential application prospects.
Nitrite-type anaerobic methane oxidation(N-DAMO)refers to the microbial biochemical process in which methane is anaerobically oxidized to carbon dioxide using nitrite as an electron acceptor.This reaction is the key link of the coupled carbon-nitrogen cycle and the new content of the microbial carbon-nitrogen cycle.Its discovery has an important contribution to the global carbon-nitrogen cycle.Based on this,this paper summarized the biochemical reaction mechanism of N-DAMO process,and summarized the influencing factors of N-DAMO bacterial activity in enrichment and culture process:when the concentration of NO_2~--N was 2.40 mmol?L~(-1),the denitrification activity of N-DAMO bacteria was the highest;the partial pressure of CH_4 was 49KPa,which could basically meet the requirement of N-DAMO bacteria for CH_4,the optimum pH value of N-DAMO bacteria was about 7.6,and the optimum temperature was about 35℃,the N-DAMO bacteria could adapt to a certain content of salinity.The distribution characteristics of N-DAMO process in different habitats such as ocean,freshwater and wetland were analyzed;On this basis,the application of coupling reaction mechanism between Anammox and N-DAMO in wastewater treatment denitrification process was discussed.This process can save a lot of material and energy,reduce economic costs and improve denitrification efficiency,and has great potential application prospects.
引文
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ETTWIG K F,BUTLER M K,PASLIER D L,et al.,2010.Nitrite-driven anaerobic methane oxidation by oxygenic bacteria[J].Nature,464(7288):543-548.
ETTWIG K F,SHIMA S,VAN P S,et al.,2008.Denitrifying bacteria anaerobically oxidize methane in the absence of Archaea[J].Environmental Microbiology,10(11):3164-73.
HE Z F,CAI C,GENG S,et al.,2013.Mdodeling a nitrite-dependent anaerobic methane oxidation process:parameters identification and model evaluation[J].Bioresource Technology,147(8):315-320.
HU B L,SHEN L D,LIAN X,et al.,2014.Evidence for nitrite-dependent anaerobic methane oxidation as a previously overlooked microbial methane sink in wetlands[J].Proceedings of the National Academy of Sciences of the United States of America,111(12):4495-4500.
HU S,ZENG R J,BUROW L C,et al.,2010.Enrichment of denitrifying anaerobic methane oxidizing microorganisms[J].Environmental Microbiology Reports,1(5):377-384.
HU S,ZENG R J,HAROON M F,et al.,2015.A laboratory investigation of interactions between denitrifying anaerobic methane oxidation(DAMO)and anammox processes in anoxic environments[J].Scientific Reports,5:8706.
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JIN R C,YANG G F,YU J J,et al.,2012.The inhibition of the Anammox process:A review[J].Chemical Engineering Journal,197(29):67-79.
KNITTEL K,BOETIUS A,2009.Anaerobic oxidation of methane:progress with an unknown process[J].Annual Review of Microbiology,63:311-334.
KNOWLES R,1982.Denitrification[J].Clinical Microbiology Reviews,46(1):43-70.
KOJIMA H,TSUTSUMI M,ISHIKAWA K,et al.,2012.Distribution of putative denitrifying methane oxidizing bacteria in sediment of a freshwater lake,Lake Biwa[J].Systematic&Applied Microbiology,35(4):233-238.
LIU Y,ZHANG J X,ZHAO L,et al.,2015.Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau[J].Applied Microbiology&Biotechnology,99(5):2371-2381.
LUESKEN F A,ALEN T A V,DER BIEZEN E V,et al.,2011a.Diversityand enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge[J].Applied Microbiology and Biotechnology,92(4):845-854.
LUESKEN F A,SANCHEZ J,ALEN T A V,et al.,2011b.Simultaneous nitrite-dependent anaerobic methane and ammonium oxidation processes[J].Applied and Environmental Microbiology,77(19):6802-6807.
RAGHOEBARSING A A,POL A,PAS-SCHOONEN K T V D,et al.,2006.A microbial consortium couples anaerobic methane oxidation to denitrification[J].Nature,440(7086):918-921.
REEBURGH W S,1976.Methane consumption in Cariaco Trench waters and sediments[J].Earth and Planetary Science Letters,28(3):337-344.
REEBURGH W S,2007.Oceanic methane biogeochemistry[J].ChemicalReviews,107(2):486-513.
SEGARRA K E A,SCHUBOTZ F,SAMARKIN V,et al.,2015.High rates of anaerobic methane oxidation in freshwater wetlands reduce potential atmospheric methane emissions[J].Nature Communications,6:7477.
SHEN L D,HU B L,LIU S,et al.,2016.Anaerobic methane oxidation coupled to nitrite reduction can be a potential methane sink in coastal environments[J].Applied Microbiology&Biotechnology,100(16):7171-7180.
SHEN L D,LIU S,ZHU Q,et al.,2014a.Distribution and diversity of nitrite-dependent anaerobic methane-oxidising bacteria in the sediments of the Qiantang River[J].Microbial Ecology,67(2):341-349.
SHEN L D,WU H S,GAO Z Q,2015.Distribution and environmental significance of nitrite-dependent anaerobic methane-oxidising bacteria in natural ecosystems[J].Applied Microbiology&Biotechnology,99(1):133-142.
SHEN L D,ZHU Q,LIU S,et al.,2014b.Molecular evidence for nitrite-dependent anaerobic methane-oxidising bacteria in the Jiaojiang Estuary of the East Sea(China)[J].Applied Microbiology&Biotechnology,98(11):5029-5038.
STROUS M,JETTEN M S M,2004.Anaerobic oxidation of methane and ammonium[J].Annual Review of Microbiology,58:99-117.
TANG KW,MCGINNIS DF,IONESCU D,et al.,2016.Methane production in oxic lake waters potentially increases aquatic methane flux to air[J].Environmental Science&Technology Letters,3(6):227-233.
THAUER R K,SHIMA S,2006.Biogeochemistry:Methane and microbes[J].Nature,440(7086):878-879.
WANG Y,ZHU G,HARHANGI H R,et al.,2012.Co-occurrence and distribution of nitrite-dependent anaerobic ammonium and methane-oxidizing bacteria in a paddy soil[J].FEMS Microbiology Letters,336(2):79-88.
WEBER H S,HABICHT K S,THAMDRUP B,2017.Anaerobic Methanotrophic Archaea of the ANME-2d Cluster Are Active in a Low-sulfate,Iron-rich Freshwater Sediment[J].Frontiers in Microbiology,8:619.
WU M L,DE VRIES S,ALEN T A V,et al.,2011b.Physiological role of the respiratory quinol oxidase in the anaerobic nitrite-reducing methanotroph‘Candidatus Methylomirabilis oxyfera’[J].Microbiology,157(Pt 3):890-898.
WU M L,ETTWIG KF,JETTEN M S M,et al.,2011a.A new intraaerobic metabolism in the nitrite-dependent anaerobic methane-oxidizing bacterium Candidatus‘Methylomirabilis oxyfera’[J].Biochemical Society Transactions,39(1):243-248.
WU M L,WILLEMS M J R,DONSELAAR E G V,et al.,2012.Ultrastructure of the denitrifying methanotroph"Candidatus Methylomirabilis oxyfera,"a novel polygon-shaped bacterium[J].Journal of Bacteriology,194(2):284-291.
XIE G J,CAI C,HU S,et al.,2017.Complete nitrogen removal from synthetic anaerobic sludge digestion liquor through integrating anammox and denitrifying anaerobic methane oxidation in a membrane biofilm reactor[J].Environmental Science&Technology,51(2):819-827.
YANG J,JIANG H C,WU G,et al.,2012.Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes[J].Frontiers of Earth Science,6(4):383-391.
ZHAO W,WANG Y,LIU S H,et al.,2013.Denitrification activities and N2O production under salt stress with varying COD/N ratios and terminal electron acceptors[J].Chemical Engineering Journal,216(2):252-260.
ZHOU L L,WANG Y,LONG X E,et al.,2014.High abundance and diversity of nitrite-dependent anaerobic methane-oxidizing bacteria in a paddy field profile[J].Fems Microbiology Letters,360(1):33-41.
ZHU G B,ZHOU L L,WANG Y,et al.,2015.Biogeographical distribution of denitrifying anaerobic methane oxidizing bacteria in Chinese wetland ecosystems[J].Environmental Microbiology Reports,7(1):128-138.
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