不同硅源对小环藻生长的影响
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摘要
硅藻是水体中主要的初级生产者,不仅是许多高营养级生物的天然饵料,还可以抑制有害藻类,如铜绿微囊藻等,并通过消耗过量的营养来帮助维持水的生态平衡。本研究以小环藻(Cyclotella sp.)为研究对象,探究了硅酸盐、微米二氧化硅及纳米二氧化硅等不同硅源对硅藻生长的影响。研究结果表明:添加纳米二氧化硅微粉及溶胶可以显著促进小环藻的生长,且其效果优于微米二氧化硅和硅酸盐。当CSI培养基中纳米二氧化硅浓度在100～200 mg/L时,对小环藻生长的促进作用最大。本研究为使用硅藻定向培养技术的应用提供了重要参考依据。
Diatoms are the main primary producers in waters, which not only feed many high-tropic aquatic organisms, but also inhibit harmful algae and help maintain the aquatic ecological balance by consuming excessive nutrients. In this study, Cyclotella sp. was used to investigate the effects of different silicon sources on the growth of diatoms, including silicate, micro-silica, and nano-silica. The results indicated that the addition of nano-silica could significantly promote the growth of Cyclotella sp, and its performance was better than micro-silica and silicate. The best promoting effects were observed when the concentration of nano-silica in CSI medium was 100～200 mg/L. This study provided important references for the application of diatom oriented cultivation technology to algal bloom control.
Diatoms are the main primary producers in waters, which not only feed many high-tropic aquatic organisms, but also inhibit harmful algae and help maintain the aquatic ecological balance by consuming excessive nutrients. In this study, Cyclotella sp. was used to investigate the effects of different silicon sources on the growth of diatoms, including silicate, micro-silica, and nano-silica. The results indicated that the addition of nano-silica could significantly promote the growth of Cyclotella sp, and its performance was better than micro-silica and silicate. The best promoting effects were observed when the concentration of nano-silica in CSI medium was 100～200 mg/L. This study provided important references for the application of diatom oriented cultivation technology to algal bloom control.
引文
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[17]Li X L,Marella T K,Tao L,et al.Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates[J].Phycol Res,2017,65(3):177-87.
[2]Paerl H W, Otten T G. Harmful cyanobacterial blooms:causes,consequences,and controls[J].Microb Ecol,2013,65(4):995-1010.
[3]Shi K,Zhang Y L,Zhou Y Q,et al.Long-term MODIS observations of cyanobacterial dynamics in Lake Taihu:Responses to nutrient enrichment and meteorological factors[J].Sci Rep,2017,7:16.
[4]Qin B Q,Zhu G W,Gao G,et al.A drinking water crisis in Lake Taihu,China:linkage to climatic variability and lake management[J].Environ Manage,2010,45(1):105-12.
[5]Treguer P,Nelson D M,Vanbennekom A J,et al.The silica balance in the world ocean:A reestimate[J].Science,1995,268(5209):375-9.
[6]谢从新．池养鲢、鳙鱼摄食习性的研究[J]．华中农业大学学报，1989(04):385-94+410.
[7]Tam N F Y,Wong Y S.Wastewater nutrient removal by Chlorella pyrenoidosa and Scenedesmus sp[J].Environ Pollut,1989,58(1):19-34.
[8]施正学．水产养殖中水色藻相变化规律初探[J]．水产养殖，2012,3335-6.
[9]李信书，阎斌伦．微藻在河蟹工厂化育苗中的作用[J]．淡水渔业，200134-5.
[10]Li X L,Marella T K,Tao L,et al.A novel growth method for diatom algae in aquaculture waste water for natural food development and nutrient removal[J].Water Sci Technol,2017,75(12):2777-83.
[11]Marella T K,Bhaskar M V,Tiwari A.A novel solution to grow diatom algae in large natural water bodies and its impact on CO2 capture and nutrient removal[J].Journal of Algal Biomass Utilization,2015,6(2):22-7.
[12]Werner D.Regulation of Metabolism by Silicate in Diatoms[M]//BENDZ G,LINDQVIST I,RUNNSTR?M-REIO V.Biochemistry of Silicon and Related Problems.Boston,MA; Springer US.1978:149-76.
[13]孙凌，金相灿，杨威，等．硅酸盐影响浮游藻类群落结构的围隔试验研究[J]．环境科学，20072174-9.
[14]李雅娟，王起华．氮、磷、铁、硅营养盐对底栖硅藻生长速率的影响[J]．大连水产学院学报，19989-16.
[15]Graham J M,Graham L E,ZulkiXy S B,et al.Freshwater diatoms as a source of lipids for biofuels[J].J Ind Microbiol Biotechnol,2012,39(3):419-28.
[16]曲青梅．舟形藻悬浮培养条件优化及营养成分分析[D]．鲁东大学，2015.
[17]Li X L,Marella T K,Tao L,et al.Optimization of growth conditions and fatty acid analysis for three freshwater diatom isolates[J].Phycol Res,2017,65(3):177-87.