NADPH氧化酶相关基因对盾壳霉代谢的影响
|
摘要
基于液质联用(LC-MS)的代谢组学分析手段,通过突变株Qnox1-6与野生株zs-1的代谢比较,研究NADPH氧化酶相关基因对盾壳霉代谢的影响。筛选得到14个重要的代谢差异物质,主要是羧酸类和苷类物质。特别是7个仅在突变株中出现的物质为:苯二酚、松柏苷、甾体皂苷类物质、3个羧酸类物质及其他物质,这些代谢物与盾壳霉寄生核盘菌紧密相关。
To investigate the metabolic effects of NOX-related genes on Coniothyrium mintans,LCMS-based metabolomics analysis was applied to detect the metabolites of mutant Qnox1-6 and the wild strain zs-1.The study shows that fourteen substances are significantly different between the two strains,most of which are carboxylic acids and glycosides.And seven metabolites only exist in the mutant strain,including hydroquinone,coniferine,saponin,three carboxylic acids and one other substance.These metabolites,which are closely related to the parasitism of C.mintans against S.sclerotiorum.
To investigate the metabolic effects of NOX-related genes on Coniothyrium mintans,LCMS-based metabolomics analysis was applied to detect the metabolites of mutant Qnox1-6 and the wild strain zs-1.The study shows that fourteen substances are significantly different between the two strains,most of which are carboxylic acids and glycosides.And seven metabolites only exist in the mutant strain,including hydroquinone,coniferine,saponin,three carboxylic acids and one other substance.These metabolites,which are closely related to the parasitism of C.mintans against S.sclerotiorum.
引文
[1]ARBONA V,IGLESIAS D J,TALON M,et al.Plant phenotype demarcation using nontargeted LC-MS and GC-MS metabolite profiling[J].J Agric Food Chem,2009,57(16):7338-7347.
[2]RAAMSDONK L M,TEUSINK B,BROADHURST D,et al.Afunctional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations[J].Nat Biotechnol,2001,19(1):45-50.
[3]GOLDMAN B S,NIERMAN W C,KAISER D,et al.Evolution of sensory complexity recorded in a myxobacterial genome[J].Proc Natl Acad Sci,2006,103(41):15200-15205.
[4]HERTWECK C.Hidden biosynthetic treasures brought to light[J].Nat Chem Biol,2009,5(7):450-452.
[5]RAMANA P,ADAMS E,AUGUSTIJINS P,et al.Metabonomics and drug development[J].Methods Mol Biol,2015,1277:195-207.
[6]KRUG D,MULLER R.Secondary metabolomics:the impact of mass spectrometry-based approaches on the discovery and characterization of microbial natural products[J].Nat Prod Rep,2014,31(6):768-783.
[7]NGUYEN Q T,MERLO M E,MEDEMA M H,et al.Metabolomics methods for the synthetic biology of secondary metabolism[J].FEBS Lett,2012,586(15):2177-2183.
[8]贺珍,胡先文,付艳苹,等.重寄生真菌盾壳霉与核盘菌对峙培养的差异代谢物分析[J].华中农业大学学报,2017,36(1):35-41.
[9]张姝,张永杰.植病生防菌盾壳霉的分子生物学研究进展[J].微生物学通报,2008,35(9):1485-1489.
[10]WEI W,ZHU W,CHENG J,et al.CmPEX6,agene involved in peroxisome biogenesis,is essential for parasitism and conidiation by the sclerotial parasite Coniothyrium minitans[J].Appl Environ Microbiol,2013,79(12):3658-3666.
[11]QIN L,GONG X,XIE J,et al.Phosphoribosylamidotransferase,the first enzyme for purine de novo synthesis,is required for conidiation in the sclerotial mycoparasite Coniothyrium minitans[J].Fungal Genet Biol,2011,48(10):956-965.
[12]YANG X,CUI H,CHENG J,et al.A HOPS protein,CmVps39,is required for vacuolar morphology,autophagy,growth,conidiogenesis and mycoparasitic functions of Coniothyrium minitans[J].Environ Microbiol,2016,18(11):3785-3797.
[13]LALUCQUE H,SILAR P.NADPH oxidase:an enzyme for multicellularity[J].Trends Microbiol,2003,11(1):9-12.
[14]WEI W,ZHU W,CHENG J,et al.Nox complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans[J/OL].Sci Rep,2016,6:24325[2019-03-31].http://www.nature.com/articles/srep24325.pdf.
[15]GOWDA H,IVANISEVIC J,JOHNSON C H,et al.Interactive XCMS online:simplifying advanced metabolomic data processing and subsequent statistical analyses[J].Anal Chem,2014,86(14):6931-6939.
[16]XIA J,WISHART D S.Using Metabo Analyst 3.0for comprehensive metabolomics data analysis[J/OL].Curr Protoc Bioinformatics,2016,55:14.10.1-14.10.91[2019-03-07].https://www.ncbi.nlm.nih.gov/pubmed/27603023.
[17]GUIJAS C,MONTENEGRO-BURKE J R,DOMINGO-AL-MENARA X,et al.METLIN:a technology platform for identifying knowns and unknowns[J].Anal Chem,2018,90(5):3156-3164.
[18]BOUHIFD M,HARTUNG T,HOGBERG H T,et al.Review:toxicometabolomics[J].J Appl Toxicol,2013,33(12):1365-1383.
[19]SHEN W,MAO H,HUANG Q,et al.Benzenediol lactones:a class of fungal metabolites with diverse structural features and biological activities[J].Eur J Med Chem,2015,97:747-777.
[20]王慧,张红歧,邬昊洋,等.筒鞘蛇菰提取物及松柏苷的抗氧化作用研究[J].三峡大学学报(自然科学版),2009(3):99-101.
[21]CUONG D M,KWON S,JEON J,etal.Identificationandcharacterization of phenylpropanoid biosynthetic genes and their accumulation in bitter melon-(Momordica charantia)[J/OL].Molecules,2018,23(2):e469[2019-03-07].https://www.ncbi.nlm.nih.gov/pubmed/29466305.doi:10.3390/molecules23020469.
[2]RAAMSDONK L M,TEUSINK B,BROADHURST D,et al.Afunctional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations[J].Nat Biotechnol,2001,19(1):45-50.
[3]GOLDMAN B S,NIERMAN W C,KAISER D,et al.Evolution of sensory complexity recorded in a myxobacterial genome[J].Proc Natl Acad Sci,2006,103(41):15200-15205.
[4]HERTWECK C.Hidden biosynthetic treasures brought to light[J].Nat Chem Biol,2009,5(7):450-452.
[5]RAMANA P,ADAMS E,AUGUSTIJINS P,et al.Metabonomics and drug development[J].Methods Mol Biol,2015,1277:195-207.
[6]KRUG D,MULLER R.Secondary metabolomics:the impact of mass spectrometry-based approaches on the discovery and characterization of microbial natural products[J].Nat Prod Rep,2014,31(6):768-783.
[7]NGUYEN Q T,MERLO M E,MEDEMA M H,et al.Metabolomics methods for the synthetic biology of secondary metabolism[J].FEBS Lett,2012,586(15):2177-2183.
[8]贺珍,胡先文,付艳苹,等.重寄生真菌盾壳霉与核盘菌对峙培养的差异代谢物分析[J].华中农业大学学报,2017,36(1):35-41.
[9]张姝,张永杰.植病生防菌盾壳霉的分子生物学研究进展[J].微生物学通报,2008,35(9):1485-1489.
[10]WEI W,ZHU W,CHENG J,et al.CmPEX6,agene involved in peroxisome biogenesis,is essential for parasitism and conidiation by the sclerotial parasite Coniothyrium minitans[J].Appl Environ Microbiol,2013,79(12):3658-3666.
[11]QIN L,GONG X,XIE J,et al.Phosphoribosylamidotransferase,the first enzyme for purine de novo synthesis,is required for conidiation in the sclerotial mycoparasite Coniothyrium minitans[J].Fungal Genet Biol,2011,48(10):956-965.
[12]YANG X,CUI H,CHENG J,et al.A HOPS protein,CmVps39,is required for vacuolar morphology,autophagy,growth,conidiogenesis and mycoparasitic functions of Coniothyrium minitans[J].Environ Microbiol,2016,18(11):3785-3797.
[13]LALUCQUE H,SILAR P.NADPH oxidase:an enzyme for multicellularity[J].Trends Microbiol,2003,11(1):9-12.
[14]WEI W,ZHU W,CHENG J,et al.Nox complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans[J/OL].Sci Rep,2016,6:24325[2019-03-31].http://www.nature.com/articles/srep24325.pdf.
[15]GOWDA H,IVANISEVIC J,JOHNSON C H,et al.Interactive XCMS online:simplifying advanced metabolomic data processing and subsequent statistical analyses[J].Anal Chem,2014,86(14):6931-6939.
[16]XIA J,WISHART D S.Using Metabo Analyst 3.0for comprehensive metabolomics data analysis[J/OL].Curr Protoc Bioinformatics,2016,55:14.10.1-14.10.91[2019-03-07].https://www.ncbi.nlm.nih.gov/pubmed/27603023.
[17]GUIJAS C,MONTENEGRO-BURKE J R,DOMINGO-AL-MENARA X,et al.METLIN:a technology platform for identifying knowns and unknowns[J].Anal Chem,2018,90(5):3156-3164.
[18]BOUHIFD M,HARTUNG T,HOGBERG H T,et al.Review:toxicometabolomics[J].J Appl Toxicol,2013,33(12):1365-1383.
[19]SHEN W,MAO H,HUANG Q,et al.Benzenediol lactones:a class of fungal metabolites with diverse structural features and biological activities[J].Eur J Med Chem,2015,97:747-777.
[20]王慧,张红歧,邬昊洋,等.筒鞘蛇菰提取物及松柏苷的抗氧化作用研究[J].三峡大学学报(自然科学版),2009(3):99-101.
[21]CUONG D M,KWON S,JEON J,etal.Identificationandcharacterization of phenylpropanoid biosynthetic genes and their accumulation in bitter melon-(Momordica charantia)[J/OL].Molecules,2018,23(2):e469[2019-03-07].https://www.ncbi.nlm.nih.gov/pubmed/29466305.doi:10.3390/molecules23020469.