辣木叶提取物的抗氧化活性研究
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摘要
[目的]研究辣木叶提取物的抗氧化活性。[方法]对辣木叶乙醇初提物用不同极性的溶剂进行萃取,得到辣木叶石油醚提取物、二氯甲烷提取物、乙酸乙酯提取物,同时对各萃取物的生物活性进行研究。[结果]辣木叶的石油醚、二氯甲烷、乙酸乙酯3个提取层对DPPH自由基均有清除作用,在相等的浓度条件下,抑制率从大到小依次为乙酸乙酯层、二氯甲烷层、石油醚层。虽然抗氧化活性较V_C弱,但浓度达500μg/mL以上时,辣木叶各层提取物均能清除90%左右的DPPH自由基。[结论]该研究为辣木叶中具有抗氧化活性的化合物的进一步分离奠定了基础,并为辣木叶开发成天然抗氧化剂或天然日化产品提供了参考依据。
[Objective]To study the antioxidant activity of extracts from Moringa oleifera leaves.[Method]The ethanol extracts from M. oleifera leaves were extracted with different organic solvents to get petroleum ether, dichloromethane and ethyl acetate extracts.The antioxidant activities of those different polarity extracts were carried out. [Result]The petroleum ether, dichloromethane and ethyl acetate extracts had certain degree of scavenging activities on DPPH radical, in the same concentration, the inhibition rate from big to small was in the order of ethyl acetate extract, dichloromethane extract, petroleum ether extract. Although their antioxidant activities were weaker than V_C, their extract can remove about 90% of DPPH radical when the concentration reached more than 500 μg/mL.[Conclusion]This study provided a foundation for the further separation of compounds with antioxidant activity in M. oleifera leaves and provided a reference for the development of M. oleifera leaves into natural antioxidants or natural daily chemicals.
[Objective]To study the antioxidant activity of extracts from Moringa oleifera leaves.[Method]The ethanol extracts from M. oleifera leaves were extracted with different organic solvents to get petroleum ether, dichloromethane and ethyl acetate extracts.The antioxidant activities of those different polarity extracts were carried out. [Result]The petroleum ether, dichloromethane and ethyl acetate extracts had certain degree of scavenging activities on DPPH radical, in the same concentration, the inhibition rate from big to small was in the order of ethyl acetate extract, dichloromethane extract, petroleum ether extract. Although their antioxidant activities were weaker than V_C, their extract can remove about 90% of DPPH radical when the concentration reached more than 500 μg/mL.[Conclusion]This study provided a foundation for the further separation of compounds with antioxidant activity in M. oleifera leaves and provided a reference for the development of M. oleifera leaves into natural antioxidants or natural daily chemicals.
引文
[1] 中国科学院《中国植物志》编辑委员会.中国植物志:第34卷第1分册 [M].北京:科学出版社,1984.
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[10] 苏瑶,赵一鹤,冯武,等.云南引种辣木籽营养成分分析与评价[J].西部林业科学,2015,44(4):142-145,149.
[11] 白旭华,黎小清,刘昌芬.超临界CO2流体萃取辣木籽油工艺研究初报[J].热带农业科技,2007,30(3):29-31.
[12] 马李一,余建兴,张重权,等.水酶法提取辣木油的工艺研究[J].林产化学与工业,2010,30(3):53-56.
[13] 陈玫,张海德,陈敏,等.几种中药不同溶剂组分的抗氧化活性研究[J].中山大学学报(自然科学版),2006,45(6):131-133.
[14] 段琼芬,马李一,余建兴.辣木油的抗紫外线性能研究[J].食品科学,2008,29(9):118-121.
[15] WATERMAN C,CHENG D M,ROJAS-SILVA P.Stable,water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro[J].Phytochemistry,2014,103:114-122.
[16] ZHAO J H,ZHANG Y L,WANG L W,et al.Bioactive secondary metabolites from Nigrospora sp.LLGLM003,an endophytic fungus of the medicinal plant Moringa oleifera Lam.[J].World J Microbiol Biotechnol,2012,28(5):2107-2112.
[2] 陈惠明.植物中的钻石——高经济价值的辣木[M].台湾:商周出版社,2003.
[3] 彭兴民,郑益兴,段琼芬,等.印度传统辣木引种栽培研究[J].热带亚热带植物学报,2008,16(6):579-585.
[4] 陆斌,宁德鲁,杜春花,等.云南的辣木引种试验初报[J].西部林业科学,2007,36(4):20-25.
[5] SHOLAPUR H N,PATIL B M.Effect of Moringa oleifera bark extracts on dexamethasone-induced insulin resistance in rats[J].Drug Res(stuttg),2013,63(10):527-531.
[6] JAISWAL D,RAI P K,MEHTA S,et al.Role of Moringa oleifera in regulation of diabetes-induced oxidative stress[J].Asian Pac J Trop Med,2013,6(6):426-432.
[7] 郑毅,解培惠,伍斌,等.辣木在金沙江干热河谷造林试验研究[J].中国农村小康科技,2011(2):52-54.
[8] 周明强,班秀文,刘清国,等.贵州辣木的引种栽培技术及特征特性研究[J].安徽农业科学,2010,38(8):4086-4088.
[9] 杨东顺,樊建麟,邵金良,等.辣木不同部位主要营养成分及氨基酸含量比较分析[J].山西农业科学,2015,43(9):1110-1115.
[10] 苏瑶,赵一鹤,冯武,等.云南引种辣木籽营养成分分析与评价[J].西部林业科学,2015,44(4):142-145,149.
[11] 白旭华,黎小清,刘昌芬.超临界CO2流体萃取辣木籽油工艺研究初报[J].热带农业科技,2007,30(3):29-31.
[12] 马李一,余建兴,张重权,等.水酶法提取辣木油的工艺研究[J].林产化学与工业,2010,30(3):53-56.
[13] 陈玫,张海德,陈敏,等.几种中药不同溶剂组分的抗氧化活性研究[J].中山大学学报(自然科学版),2006,45(6):131-133.
[14] 段琼芬,马李一,余建兴.辣木油的抗紫外线性能研究[J].食品科学,2008,29(9):118-121.
[15] WATERMAN C,CHENG D M,ROJAS-SILVA P.Stable,water extractable isothiocyanates from Moringa oleifera leaves attenuate inflammation in vitro[J].Phytochemistry,2014,103:114-122.
[16] ZHAO J H,ZHANG Y L,WANG L W,et al.Bioactive secondary metabolites from Nigrospora sp.LLGLM003,an endophytic fungus of the medicinal plant Moringa oleifera Lam.[J].World J Microbiol Biotechnol,2012,28(5):2107-2112.