腐皮镰孢菌和可可毛色二孢菌对白木香结香木质部化学成分的影响研究
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摘要
研究腐皮镰孢菌和可可毛色二孢菌对白木香的浸染结香效果,采用单一菌处理(1g/L、3 g/L)、混合菌处理(每个菌0.5 g/L、1 g/L、1.5 g/L),以输液方式浸染5个月后,对其木质部采用GC-MS联用仪开展化学成分分析。结果表明,检测出的77种化学成分含有芳香族化合物、倍半萜类化合物、脂肪酸和其他物质;与单菌侵染相比,混合菌侵染白木香树更能促使沉香特征性成分产生,接菌量为3 g时效果最佳;可可毛色二孢菌结香效果稍好于腐皮镰孢菌;结香效果与侵染菌的量存在一定关系。
To study the effects of Fusarium solani and Lasiodiplodia theobromae on the agarwood formation of Aquilaria sinensis,the single bacterium(1 g/L,3 g/L) and the mixed bacteria(0.5 g/L,1 g/L,1.5 g/L for each bacteria) were used to infuse the Aquilaria sinensis and analyzed the chemical components of the xylem after 5 months by GC-MS. The results showed that 77 chemical components were detected containing aromatic compounds,sesquiterpenoids and fatty acids. The effect of the mixed bacteria was better than that of the single bacterium on agarwood formation which was the best with 3 g mixed bacteria. The effect of Lasiodiplodia theobromae was slightly better than that of Fusarium solani. There was a relationship between the effect and the quantity of bacteria.
To study the effects of Fusarium solani and Lasiodiplodia theobromae on the agarwood formation of Aquilaria sinensis,the single bacterium(1 g/L,3 g/L) and the mixed bacteria(0.5 g/L,1 g/L,1.5 g/L for each bacteria) were used to infuse the Aquilaria sinensis and analyzed the chemical components of the xylem after 5 months by GC-MS. The results showed that 77 chemical components were detected containing aromatic compounds,sesquiterpenoids and fatty acids. The effect of the mixed bacteria was better than that of the single bacterium on agarwood formation which was the best with 3 g mixed bacteria. The effect of Lasiodiplodia theobromae was slightly better than that of Fusarium solani. There was a relationship between the effect and the quantity of bacteria.
引文
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[5] QI S Y,LU B Y,ZHU L F,et al.Formation of oxo-agarospi-rol inAquliaria sinensis[J].Plant Phys Commun,1992(28):336-339.
[6] NG L T,CHANG Y S,KADIR A.A review on agar (gaharu)producingAquilariaspecies [J].J Trop For Products,1997,2(2):272-285.
[7] 林峰,戴好富,王辉,等.两批接菌法所产沉香挥发油化学成分的气相色谱-质谱联用分析[J].时珍国医国药,2010,21(8):1901-1902.
[2] BOSE S R.Enzymes of wood-rotting fungi [J].Ergeb Enz-ymforsch,1939(8):267-276.
[3] SUBEHAN,UEDA J Y,FUJINO H,et al.A field survey of agarwood in Indonesia [J].J Trad Med,2005,22(4):244-251.
[4] GIBSON I A S.The role of fungi in the origin of oleoresin de-posits (Agaru) in the wood ofAquillaria agallocha(Roxb.)[J].Bano Biggyn Patrika,1977,6(1):16-26.
[5] QI S Y,LU B Y,ZHU L F,et al.Formation of oxo-agarospi-rol inAquliaria sinensis[J].Plant Phys Commun,1992(28):336-339.
[6] NG L T,CHANG Y S,KADIR A.A review on agar (gaharu)producingAquilariaspecies [J].J Trop For Products,1997,2(2):272-285.
[7] 林峰,戴好富,王辉,等.两批接菌法所产沉香挥发油化学成分的气相色谱-质谱联用分析[J].时珍国医国药,2010,21(8):1901-1902.