根分泌物有机酸的研究方法
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摘要
根分泌物主要是以碳为基础的低分子化合物和高分子化合物。植物分泌的有机酸主要有乙酸、草酸、柠檬酸、苹果酸、酒石酸和琥珀酸等,它们调节细胞代谢,为根际和微生物提供营养。植物根系分泌有机酸的研究对于了解植物的根际效应具有重要的学术意义。简述了根系分泌物有机酸的收集、分离和检测方法。
Root exudates include low molecular compounds, high molecular compounds, and primarily carbon-based compounds. The organic acids secreted by plants are mainly acetic acid, oxalic acid, citric acid, malic acid, tartaric acid and succinic acid, which regulate cell metabolism and provide nutrients for the rhizosphere and microorganisms. The study of plant root exudates of organic acids has important academic significance for understanding the rhizosphere effects of plants. This paper briefly described the methods of collection, separation and detection of organic acids in root exudates.
Root exudates include low molecular compounds, high molecular compounds, and primarily carbon-based compounds. The organic acids secreted by plants are mainly acetic acid, oxalic acid, citric acid, malic acid, tartaric acid and succinic acid, which regulate cell metabolism and provide nutrients for the rhizosphere and microorganisms. The study of plant root exudates of organic acids has important academic significance for understanding the rhizosphere effects of plants. This paper briefly described the methods of collection, separation and detection of organic acids in root exudates.
引文
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[4]Zheng Y,Zhang F S.Effect of soil moisture on release of low-molecular-weight organic acids in root exudates and the accumulation of iron in root apoplasm of peanut[J].Pedosphere,2000,10(4):333-338.
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[10]林琦,陈英旭,陈怀满,等.根系分泌物与重金属的化学行为研究[J].植物营养与肥料学报,2003,9(4):425-431.
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[13]黄鹤,赵月春,郭秀兰.大豆在铝胁迫下分泌的柠檬酸与品种的耐酸铝性无关[J].大豆科学,2003,22(3):218-222.
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[15]Tu S X,Ma L,Luongo T.Root exudates and arsenic accumulation in arsenic hyperaccumulating Pteris vittata and non-hypreraccumulating Nephrolepis exaltata[J].Plant and Soil,2004(258):9-19.
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[24]王树起,韩晓增.乔云发,等.缺磷胁迫条件下大豆根系有机酸的分泌特性[J].大豆科学,2009,28(3):409-414.
[25]徐根娣,金婷婷,刘鹏,等.铝胁迫下大豆根系分泌有机酸和氨基酸的特性[J].生态环境,2008,17(3):1204-1209.
[26]施积炎,陈英旭,林琦,等.根分泌物与微生物对污染土壤重金属活性的影响[J].中国环境科学,2004,24(3):316-319.
[27]李德华,贺立源,李建生,等.一种根系分泌物中有机酸的前处理和高效液相色谱检测方法[J].植物生理学通讯,2004,40(2):219-222.
[28]Neumann G,R?mheld V.Root excretion of carboxylic acids and protons in phosphorus-deficient plants [J].Plant and Soil,1999(211):121-130.
[29]贾黎明,冯菊芬,文学军,等.循环水根系分泌物收集技术的研究及应用[J].北京林业大学学报,2003,25(6):6-10.
[30]廖继佩,林先贵,曹志洪,等.一种新型连续根分泌物收集装置[J].土壤,2003,35(4):311-313.
[31]王占义,潘宁,罗茜,等.一种新型根系分泌物收集装置与收集方法的介绍[J].土壤学报,2010,47(4):747-752.
[32]罗小三,仓龙,郝秀珍,等.原位土壤溶液采样及可溶性有机碳(DOC)的紫外吸收光谱直接测定探讨[J].土壤,2007,39(6):943-947.
[33]陈海峰,周东美,仓龙.垂直电场对重金属络合物在土壤中迁移过程的影响[J].中国环境科学,2006(26):78-82.
[34]He Y,Xu J M,Tang C X,et al.Facilitation of pentachlorophenol degradation in the rhizosphere of ryegrass (Lolium perenne L.) [J].Soil Biology & Biochemistry,2005,37(11):2017–2024.
[35]He Y,Xu J M,Ma Z H,et al.Proling of PLFA:Implications for nonlinear spatial gradient of PCP degradation in the vicinity of Lolium perenne L.roots [J].Soil Biology & Biochemistry,2007,39(5):1121-1129.
[36]He Y,Xu J M,Lv X F,et al.Does the depletion of pentachlorophenol in root–soil interface follow a simple linear dependence on the distance to root surfaces[J].Soil Biology & Biochemistry,2009,41(9):1807-1813.
[37]Muratova A,Golubev S,Wittenmayer L,et al.Effect of the polycyclic aromatic hydrocarbon phenanthrene on root exudation of Sorghum bicolor (L.) Moench[J].Environmental and Experimental Botany,2009,66(3):514-521.
[38]尤江峰,候宁宁,徐睦芸,等.铝胁迫下大豆根系脱落酸累积与柠檬酸分泌的关系研究[J].植物营养与肥料学报,2010,16(4):899-905.
[39]李学淳,杨忠,李亚东.越橘根系分泌物中有机组分及动态变化[J].吉林农业大学学报,2009,31(5):578-580.
[40]杨建峰,贺立源,左雪东,等.有潜在性的大孔吸附树脂模拟植物根系分泌有机酸吸附的方法[J].华北农学报,2007,22(3):127-131.
[41]Ma J F,Zheng S J,Matsumoto H.Specific secretion of citric acid induced by Al stress in Cassia tora L [J].Plant and Cell Physiol,1997,38(9):1019-1025.
[42]王敏,屈峰,林金明.毛细管电泳在低分子量有机酸分析中的应用[J].分析科学学报,2005,21(4):454-458.
[43]俞乐,彭新湘,杨崇,等.反相高效液相色潽法测定植物组织及根分泌物中的草酸[J].分析化学研究简报,2002,30(9):1119-1122.
[44]邓云,官会林,戴开结,等.不同供磷水平对云南松幼苗形态建成及根际有机酸分泌的影响[J].云南大学学报(自然科学版),2006,28(4):358-363.
[45]王君,王东升.大豆、小麦根系分泌物中低分子量有机酸分析方法[J].辽宁工程技术大学学报(自然科学版),2009(28):240-242.
[46]谢明吉,严重玲,叶箐.菲对黑麦草根系几种低分子量分泌物的影响[J].生态环境,2008,17(2):576-579.
[47]柴强,黄高宝,黄鹏,等.鹰咀豆根系分泌物的分离鉴定及典型分泌物苯甲醛的化感效应[J].草业学报,2005,14(1):106-111.
[48]Wang H B,Wong W H,Lan C Y,et al.Organic acids in two arsenic hyperaccumulators and a non-hyperaccumulator of Pteris exposed to elevated arsenic concentrations[J].International Journal of Environmental Analytical Chemistry,2011,91(3):241-254.
[49]Westergaard B,Hansen H C B.Determination of anions in soil solutions by capillary zone electrophoresis[J].Analyst,1998,123(4):721-724.
[50]Malá Z,Vespalec .Capillary zone electrophoresis with indirect photometric detection in the visible range[J].Electrophoresis,1994,15(1):1526-1530.
[2]Nguyen C.Rhizodeposition of organic C by plants:mechanisms and controls [J].Agronomy for Sustainable Development,2003(23):375-396.
[3]Chen S T,Huang Y.Determination of respiration,gross nitrification and denitrification in soil profile using BaPS system[J].Journal of Environmental Sciences,2006,18(5):937-943.
[4]Zheng Y,Zhang F S.Effect of soil moisture on release of low-molecular-weight organic acids in root exudates and the accumulation of iron in root apoplasm of peanut[J].Pedosphere,2000,10(4):333-338.
[5]Graham J H,Leonard RT,Menge J A.Membrane-mediated decrease in root exudation responsible for phorphorus inhibition of vesicular-arbuscular mycorrhiza formation [J].Plant Physiol,1981(68):548-552.
[6]Dayakra V,Badri,Jorge M,Vivanco.Regulation and function of root exudates[J].Plant,Cell and Environment,2009(32):666-681.
[7]陆松柳,胡洪营,孙迎雪,等.3种湿地植物在水培条件下的生长状况及根系分泌物研究[J].环境科学,2009,30(7):1901-1905.
[8]McGrath S P,Shen Z G,Zhao F J.Heavy metal uptake and chemical changes in the rhizosphere of Thlaspi caerulescens and Thlaspi ochroleucum grown in contaminated soils[J].Plant and Soil,1997,188(1):153-159.
[9]Hammer D,Keller C.Change in the rhizosphere of metal-accumulating plants evidenced by chemical extractants[J].Journal of Environmental Quality,2002(31):1561-1569.
[10]林琦,陈英旭,陈怀满,等.根系分泌物与重金属的化学行为研究[J].植物营养与肥料学报,2003,9(4):425-431.
[11]MA J F.Role of organic acid in detoxification of aluminum in higher plants[J].Plant and Cell Physiology,2000,41(4):383-390.
[12]Shen H,He L F,Sasaki T,et al.Citrate secretion coupled with the modulation of soybean root tip under aluminum stress,Up-regulation of transcription,translation,and threonine-oriented phosphorylation of plasma membrane H+-ATPase[J].Plant Physiology,2005,138(1):287-296.
[13]黄鹤,赵月春,郭秀兰.大豆在铝胁迫下分泌的柠檬酸与品种的耐酸铝性无关[J].大豆科学,2003,22(3):218-222.
[14]Silva I R,Smyth T J,Raper C D,et al.Differential aluminum tolerance in soybean:an evaluation of the role for organic acid[J].Physiologia Plantarum,2001,112(2):200-210.
[15]Tu S X,Ma L,Luongo T.Root exudates and arsenic accumulation in arsenic hyperaccumulating Pteris vittata and non-hypreraccumulating Nephrolepis exaltata[J].Plant and Soil,2004(258):9-19.
[16]陈英旭,林琦,陆芳,等.有机酸对铅、镉植株危害的解毒作用研究[J].环境科学学报,2007,20(4):467-472.
[17]徐婉明,张志红,雷艳宜,等.气质联用方法测定香蕉根系分泌物[J].热带作物学报,2010,31(8):1403-1408.
[18]El-Halmouch Y,Benharrat H,Thalouarn P.Effect of root exudates from different tomato genotypes on broomrape (O.aegyptiaca) seed germination and tubercle development[J].Crop Protection,2006,25(5):501-507.
[19]Marscher H.Mineral in High Plant[M].London:Academic Press,1995:23-50.
[20]Muratova A,Pozdnyakova N,Golubev S,et al.Oxidoreductase activity of sorghum root exudates in a phenanthrene-contaminated environment[J].Chemosphere,2009,74(8):1031-1036.
[21]李勇,黄小芳,丁万隆.营养元素亏缺对人参根分泌物主要成分的影响[J].应用生态学报,2008,19(8):1688-1693.
[22]田中民,秦芳玲,王波.缺磷白羽扇豆根系分泌物收集方法的比较研究[J].西北农林科技大学学报(自然科学版),2003,31(4):154-158.
[23]王文斌,吴小平,尹玉莲,等.水培条件下橡胶树幼苗根系分泌物的GC-MS分析[J].热带作物学报,2010,31(12):2281-2286.
[24]王树起,韩晓增.乔云发,等.缺磷胁迫条件下大豆根系有机酸的分泌特性[J].大豆科学,2009,28(3):409-414.
[25]徐根娣,金婷婷,刘鹏,等.铝胁迫下大豆根系分泌有机酸和氨基酸的特性[J].生态环境,2008,17(3):1204-1209.
[26]施积炎,陈英旭,林琦,等.根分泌物与微生物对污染土壤重金属活性的影响[J].中国环境科学,2004,24(3):316-319.
[27]李德华,贺立源,李建生,等.一种根系分泌物中有机酸的前处理和高效液相色谱检测方法[J].植物生理学通讯,2004,40(2):219-222.
[28]Neumann G,R?mheld V.Root excretion of carboxylic acids and protons in phosphorus-deficient plants [J].Plant and Soil,1999(211):121-130.
[29]贾黎明,冯菊芬,文学军,等.循环水根系分泌物收集技术的研究及应用[J].北京林业大学学报,2003,25(6):6-10.
[30]廖继佩,林先贵,曹志洪,等.一种新型连续根分泌物收集装置[J].土壤,2003,35(4):311-313.
[31]王占义,潘宁,罗茜,等.一种新型根系分泌物收集装置与收集方法的介绍[J].土壤学报,2010,47(4):747-752.
[32]罗小三,仓龙,郝秀珍,等.原位土壤溶液采样及可溶性有机碳(DOC)的紫外吸收光谱直接测定探讨[J].土壤,2007,39(6):943-947.
[33]陈海峰,周东美,仓龙.垂直电场对重金属络合物在土壤中迁移过程的影响[J].中国环境科学,2006(26):78-82.
[34]He Y,Xu J M,Tang C X,et al.Facilitation of pentachlorophenol degradation in the rhizosphere of ryegrass (Lolium perenne L.) [J].Soil Biology & Biochemistry,2005,37(11):2017–2024.
[35]He Y,Xu J M,Ma Z H,et al.Proling of PLFA:Implications for nonlinear spatial gradient of PCP degradation in the vicinity of Lolium perenne L.roots [J].Soil Biology & Biochemistry,2007,39(5):1121-1129.
[36]He Y,Xu J M,Lv X F,et al.Does the depletion of pentachlorophenol in root–soil interface follow a simple linear dependence on the distance to root surfaces[J].Soil Biology & Biochemistry,2009,41(9):1807-1813.
[37]Muratova A,Golubev S,Wittenmayer L,et al.Effect of the polycyclic aromatic hydrocarbon phenanthrene on root exudation of Sorghum bicolor (L.) Moench[J].Environmental and Experimental Botany,2009,66(3):514-521.
[38]尤江峰,候宁宁,徐睦芸,等.铝胁迫下大豆根系脱落酸累积与柠檬酸分泌的关系研究[J].植物营养与肥料学报,2010,16(4):899-905.
[39]李学淳,杨忠,李亚东.越橘根系分泌物中有机组分及动态变化[J].吉林农业大学学报,2009,31(5):578-580.
[40]杨建峰,贺立源,左雪东,等.有潜在性的大孔吸附树脂模拟植物根系分泌有机酸吸附的方法[J].华北农学报,2007,22(3):127-131.
[41]Ma J F,Zheng S J,Matsumoto H.Specific secretion of citric acid induced by Al stress in Cassia tora L [J].Plant and Cell Physiol,1997,38(9):1019-1025.
[42]王敏,屈峰,林金明.毛细管电泳在低分子量有机酸分析中的应用[J].分析科学学报,2005,21(4):454-458.
[43]俞乐,彭新湘,杨崇,等.反相高效液相色潽法测定植物组织及根分泌物中的草酸[J].分析化学研究简报,2002,30(9):1119-1122.
[44]邓云,官会林,戴开结,等.不同供磷水平对云南松幼苗形态建成及根际有机酸分泌的影响[J].云南大学学报(自然科学版),2006,28(4):358-363.
[45]王君,王东升.大豆、小麦根系分泌物中低分子量有机酸分析方法[J].辽宁工程技术大学学报(自然科学版),2009(28):240-242.
[46]谢明吉,严重玲,叶箐.菲对黑麦草根系几种低分子量分泌物的影响[J].生态环境,2008,17(2):576-579.
[47]柴强,黄高宝,黄鹏,等.鹰咀豆根系分泌物的分离鉴定及典型分泌物苯甲醛的化感效应[J].草业学报,2005,14(1):106-111.
[48]Wang H B,Wong W H,Lan C Y,et al.Organic acids in two arsenic hyperaccumulators and a non-hyperaccumulator of Pteris exposed to elevated arsenic concentrations[J].International Journal of Environmental Analytical Chemistry,2011,91(3):241-254.
[49]Westergaard B,Hansen H C B.Determination of anions in soil solutions by capillary zone electrophoresis[J].Analyst,1998,123(4):721-724.
[50]Malá Z,Vespalec .Capillary zone electrophoresis with indirect photometric detection in the visible range[J].Electrophoresis,1994,15(1):1526-1530.