饲料中铬检测方法的改进研究
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摘要
目的分析饲料中铬检测方法存在的问题、原因,并对检测方法进行改进。方法用10 mL 6 mol/L的盐酸溶液处理灰化后的样品,所得样液用原子吸收火焰法进行测定,并与国标法中用20%的硝酸溶液处理灰化后的样品的测定结果进行比较。结果按照国家标准方法,饲料中铬检测检测结果明显低于用10mL6mol/L的盐酸溶液进行前处理的改进方法,原因是饲料中铬检测标准的前处理过程未能充分解离、提取饲料样品中的铬,而经改进后的方法,使用6 mol/L的盐酸溶液并增加酸溶液用量,可以充分溶解提取样品中的铬。结论 2种方法测定结果差异明显,改进后的方法测定结果更准确。
Objective To analyze the problems and causes of chromium detection in feed, and improve the detection methods. Methods The samples were treated with 10 m L 6 mol/L hydrochloric acid solution after ashing, and determined by atomic absorption spectrophotometer flame method, and the results were compared with those obtained from ashing samples treated with 20% nitric acid solution in GB method. Results The results of the national standard method for the determination of chromium in feed were significantly lower than those of the improved method for the treatment of grated samples with 10 mL 6 mol/L hydrochloric acid solution. The reason was that the standard pretreatment process of chromium detection in feed failed to fully dissociate and e xtract chromium from feed samples. However, in the improved method, 6 mol/L hydrochloric acid solution and the amount of acid solution were increased to fully dissolve and extract chromium from the samples. Conclusion The results of the 2 methods are significantly different, and the improved method is more accurate.
Objective To analyze the problems and causes of chromium detection in feed, and improve the detection methods. Methods The samples were treated with 10 m L 6 mol/L hydrochloric acid solution after ashing, and determined by atomic absorption spectrophotometer flame method, and the results were compared with those obtained from ashing samples treated with 20% nitric acid solution in GB method. Results The results of the national standard method for the determination of chromium in feed were significantly lower than those of the improved method for the treatment of grated samples with 10 mL 6 mol/L hydrochloric acid solution. The reason was that the standard pretreatment process of chromium detection in feed failed to fully dissociate and e xtract chromium from feed samples. However, in the improved method, 6 mol/L hydrochloric acid solution and the amount of acid solution were increased to fully dissolve and extract chromium from the samples. Conclusion The results of the 2 methods are significantly different, and the improved method is more accurate.
引文
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[13]王长芹,张凯,邓艺,等.微波消解-原子吸收法测定土壤中的铅和镉[J].济宁医学院学报,2015,38(2):124-128.Wang CQ,Zhang K,Deng Y,et al.Determination of lead and cadmium in soil by microwave digestion atomic absorption spectrometry[J].J Jining Med Coll,2015,38(2):124-128.
[14]冯素玲,李晶,李全民.原子吸收分析中的氘灯背景校正技术原理[J].大学化学,2011,26(3):65-67.Feng SL,Li J,Li QM.Principle of background correction for deuterium lamp in atomic absorption spectrometry[J].J Univ Chem,2011,26(3):65-67.
[15]李云,魏敏,高庆军,等.饲料中钙对铅含量测定的影响[J].中国农业科技导刊,2009,11(S1):24-27.Li Y,Wei M,Gao QJ,et al.Effect of calcium in feed on determination of lead content[J].J Agric Sci Technol,2009,11(S1):22-23.
[16]梁兆斌,韦衢.微波消解-原子吸收法测定饲料中Pb、Cd、Cu、Zn含量[J].畜牧兽医科技信息,2015,6:32-34.Liang ZB,Wei Q.Determination of Pb,Cd,Cu and Zn in feed by microwave digestion atomic absorption spectrometry[J].J Chin J Anim Husb Vet Med,2015,6:32-34.
[2]张美琴,侯晨美,陈海仟,等.微波消解样品-石墨炉原子吸收光谱法测定饲料中铬[J].理化检验-化学分册,2010,46(9):1014-1017.Zhang MQ,Hou CM,Chen HQ,et al.Determination of chromium in feed by graphite furnace atomic absorption spectrometry after microwave digestion[J].Phys Test Chem Anal B,2010,46(9):1014-1017.
[3]曹珺,赵丽娇,钟儒刚.原子吸收光谱法测定食品中重金属含量的研究进展[J].食品科学,2012,33(7):304-309.Cao J,Zhao LJ,Zhong RG,et al.Progress in determination of heavy metals in foods by atomic absorption spectrometry[J].J Food Sci,2012,33(7):304-309.
[4]黄文水.原子吸收光谱法测定饲料中铬和锌[J].光谱实验室,2006,23(2):363-365.Huang WS.Determination of chromium and zinc in animal feed by atomic adsorption spectrometry[J].J Chin J Spectrosc Lab,2006,23(2):363-365.
[5]GB/T 13088-2006饲料中铬的测定[S].GB/T 13088-2006 Determination of chromium in feeds[S].
[6]GB/T 13885-2017饲料中钙、铜、铁、镁、锰、钾、钠和锌含量的测定原子吸收光谱法[S].GB/T 13088-2006 Determination of calcium,copper,iron,magnesium,manganese,potassium,sodium and zinc in feeds atomic absorption spectrometry[S].
[7]廖佳佳,胡宏灿.原子吸收分光光度法测定饲料原料铜、锰、锌、铅的两种前处理方法比较研究[J].化工工程与装备,2008,(4):126-128.Liao JJ,Hu HC.Comparative study on two pretreatment methods for determination of copper,Manganese,Zinc and Lead in feed materials by atomic absorption spectrophotometry[J].J Chem Eng Equip,2008,(4):126-128.
[8]孟凡胜.饲料中部分成分对石墨炉原子吸收法测定铅的影响[J].中国农业科技导报,2009,11(S1):22-23.Meng FS.Effect of some components in feed on determination of lead by graphite furnace atomic absorption spectrometry[J].J Agric Sci Technol,2009,11(S1):22-23.
[9]许文艳,李晓蓉,郭斌.原子吸收光谱法检测技术研究综述[J].甘肃农业科技,2015,(11):76-78.Xu WY,Li XR,Guo B.Summary of research on atomic absorption spectrometry[J].J Gansu Agric Sci Technol,2015,11:76-78.
[10]贾涛.原子吸收光谱法检测饲料添加剂硫酸锰中铅含量的探讨[J].饲料广角,2009,(19):40-47.Jia T.Effects of clenbuterol on levels of ATP and antioxidant capacity of myocardium in mice[J].Feed China,2009,(19):40-47.
[11]贾涛,方芳,郑君杰,等.关于牛奶中重金属元素铅与铬检测方法的改进与探讨[J].中国乳业,2017,(185):62-66.Jia T,Fang F,Zheng JJ,et al.Improvement and discussion on detection method of heavy metals lead and chromium in milk[J].J China Dairy,2017,185:62-66.
[12]喻志瑶,侯丽华,闫丽丽,等.皮革中六价铬检测方法研究进展[J].西部皮革,2017,(3):29-31.Yu ZY,Hou LH,Y LL,et al.Research progress of detection methods for six valent chromium in leather[J].J West Leather,2017,3:29-31.
[13]王长芹,张凯,邓艺,等.微波消解-原子吸收法测定土壤中的铅和镉[J].济宁医学院学报,2015,38(2):124-128.Wang CQ,Zhang K,Deng Y,et al.Determination of lead and cadmium in soil by microwave digestion atomic absorption spectrometry[J].J Jining Med Coll,2015,38(2):124-128.
[14]冯素玲,李晶,李全民.原子吸收分析中的氘灯背景校正技术原理[J].大学化学,2011,26(3):65-67.Feng SL,Li J,Li QM.Principle of background correction for deuterium lamp in atomic absorption spectrometry[J].J Univ Chem,2011,26(3):65-67.
[15]李云,魏敏,高庆军,等.饲料中钙对铅含量测定的影响[J].中国农业科技导刊,2009,11(S1):24-27.Li Y,Wei M,Gao QJ,et al.Effect of calcium in feed on determination of lead content[J].J Agric Sci Technol,2009,11(S1):22-23.
[16]梁兆斌,韦衢.微波消解-原子吸收法测定饲料中Pb、Cd、Cu、Zn含量[J].畜牧兽医科技信息,2015,6:32-34.Liang ZB,Wei Q.Determination of Pb,Cd,Cu and Zn in feed by microwave digestion atomic absorption spectrometry[J].J Chin J Anim Husb Vet Med,2015,6:32-34.