褐环乳牛肝菌(Suillus luteus)对樟子松耐盐性的影响
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摘要
为研究外生菌根真菌褐环乳牛肝菌(Suillus luteus)对樟子松苗木耐盐性的影响,采用打孔灌根的接种方法对樟子松苗木接种褐环乳牛肝菌,用盆栽试验研究不同含盐量(0.1g·kg-1和0.2g·kg-1NaCl)胁迫10d后褐环乳牛肝菌对樟子松苗木的菌根侵染情况以及樟子松苗木针叶电导率、丙二醛、保护酶活性、可溶性糖、可溶性蛋白、脯氨酸等生理指标的变化,以相同浓度盐胁迫下不接种褐环乳牛肝菌的樟子松苗木为对照。结果表明:试验选用的不同浓度NaCl胁迫,并未影响樟子松外生菌根的合成。接种褐环乳牛肝菌樟子松苗木不同盐浓度处理间苗高、地径、鲜重、干重差异不显著,与不接种樟子松苗木盐胁迫的苗高、鲜重、干重差异显著,地径无显著性差异。接种褐环乳牛肝菌樟子松苗木针叶的相对电导率低于不接种苗木,盐胁迫下接种处理与不接种处理间差异显著;盐胁迫下不接种苗木丙二醛含量高于接种苗木92%以上;盐处理中,接种褐环乳牛肝菌樟子松苗木过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性均高于不接种处理,增幅分别为10.70%～407.69%,84.45%～158%,4.79%～8.57%;盐胁迫增加了苗木可溶性糖、可溶性蛋白含量,可溶性糖增加13.20%～29.53%,可溶性蛋白增加9.64%～13.43%;接种处理降低了苗木脯氨酸含量,降低幅度为18.24%～25.30%。可见,外生菌根菌褐环乳牛肝菌与樟子松形成菌根后提高了樟子松苗木对NaCl的耐受性。
To study the effect of Suillus luteus on salt resistance of Pinus sylvestris var. mongolica seedlings, a greenhouse experiment was carried out by drilling and root irrigation with or without Suillus luteus at 2 NaCl levels(0.1 g·kg-1and 0.2 g·kg-1).After 10 d, the mycorrhizal infection status was observed, and the physiological index changes as needles electrical conductivity,malondialdehyde, protective enzyme activities, contents of soluble sugar, soluble protein, and proline were recorded. The result showed that the mycorrhizal synthesis was not affected by different salt stress. And after Suillus luteus inoculation, there were no significant differences between the two NaCl levels on seedlings height, dry and fresh weight and ground diameter. Compared with non-inoculated seedlings, those indexs had significant differences except ground diameter. Under salt stress, needles electrical conductivity was significantly declined after inoculation. The content of malondialdehyde was 92% higher than non-inoculated seedlings. The CAT, POD, SOD activity were 10.7%-407.69%, 84.45%-158%, 4.79%-8.57% higher than non-inoculated seedlings respectively. The content of soluble sugar and soluble protein respectively increased 13.20%-29.53%, 9.64%-13.43% under salt stress. The inoculation decreased proline content by 18.24%-25.30%. The results presented here indicate that the mycorrhiza synthetised from Suillus luteus and the seedlings could improve seedlings salt resistance.
To study the effect of Suillus luteus on salt resistance of Pinus sylvestris var. mongolica seedlings, a greenhouse experiment was carried out by drilling and root irrigation with or without Suillus luteus at 2 NaCl levels(0.1 g·kg-1and 0.2 g·kg-1).After 10 d, the mycorrhizal infection status was observed, and the physiological index changes as needles electrical conductivity,malondialdehyde, protective enzyme activities, contents of soluble sugar, soluble protein, and proline were recorded. The result showed that the mycorrhizal synthesis was not affected by different salt stress. And after Suillus luteus inoculation, there were no significant differences between the two NaCl levels on seedlings height, dry and fresh weight and ground diameter. Compared with non-inoculated seedlings, those indexs had significant differences except ground diameter. Under salt stress, needles electrical conductivity was significantly declined after inoculation. The content of malondialdehyde was 92% higher than non-inoculated seedlings. The CAT, POD, SOD activity were 10.7%-407.69%, 84.45%-158%, 4.79%-8.57% higher than non-inoculated seedlings respectively. The content of soluble sugar and soluble protein respectively increased 13.20%-29.53%, 9.64%-13.43% under salt stress. The inoculation decreased proline content by 18.24%-25.30%. The results presented here indicate that the mycorrhiza synthetised from Suillus luteus and the seedlings could improve seedlings salt resistance.
引文
[1]杨松杰,张富春,刘世贵.盐渍化土壤改良利用新方法—植物耐盐基因的遗传转化[J].世界林业研究,2006,19(1):14-19.
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[5]盛敏,唐明,张峰峰,等.盐胁迫下接种AM真菌对玉米耐盐性的影响[J].西北植物学报,2011,31(2):332-337.
[6]贺学礼,赵丽莉,李英鹏.NaCl胁迫下AM真菌对棉花生长和叶片保护酶系统的影响[J].生态学报,2005,25(1):188-193.
[7] MUHSIN T M,ZWIAZEK T J.Colonization with Heleloma crustuliniform increases water conductance and limits shoot sodium uptake in white spruce(Picea glauca)seedlings[J].Plant and Soil,2002,23(8):217-225.
[8] CALVO POLANCO M,ZWIAZEK JJ,VOICU MC.Responses of ectomycorrhizal American elm(Ulmus americana)seedlings to salinity and soil compaction[J].Plant Soil,2008,308(1-2):189-200.
[9] BANDOU E,LEBAILLY F,MULLER F,et al.The ectomycorrhizal fungus Scleroderma bermudense alleviates salt stress in seagrape(Coccoloba uvifera L.)seedlings[J].Mycorrhiza,2006,16(8):559-565.
[10]黄艺,姜学艳,梁振春,等.盐胁迫下外生菌根真菌对油松生长及生理的影响[J].农业环境科学学报,2006,25(6):1475-1480.
[11]黄艺,姜学艳,梁振春,等.外生菌根真菌接种和施磷对油松幼苗抗盐性的影响[J].生态环境,2004,13(4):622-625.
[12]张峰峰.甘肃盐碱土植物菌根真菌资源与耐盐性研究[D].杨凌:西北农林科技大学,2007.
[13]李小利.外生菌根真菌菌株耐盐碱性筛选以及黑松(Pinm thunbergii Pari.)菌根苗合成、耐盐碱性研究[D].南京:南京农业大学,2013.
[14]范克胜,吴小芹,任嘉红,等.盐胁迫下外生菌根真菌与根际有益细菌互作对杨树光合特性的影响[J].西北植物学报,2011,31(6):1216-1222.
[15]刘强,闫伟.过量钠盐、碱性pH值对褐环乳牛肝菌生长的影响[J].内蒙古农业大学学报,2009,30(2):52-56.
[16]刘强.褐环乳牛肝菌不同菌株及其与油松合成的菌根苗对盐碱的适应性[D].呼和浩特:内蒙古农业大学,2009.
[17]宋小双,邓勋,尹大川,等.褐环乳牛肝菌N94与绿木霉T43复合接种下红皮云杉苗木的生理响应[J].吉林农业大学学报,2015,37(1):37-42,46.
[18]尹大川,杨立宾,邓勋,等.绿木霉对樟子松苗木生长指标及生理生化指标的影响[J].北京林业大学学报,2015,37(1):78-83.
[19]尹大川,邓勋,CHET I,等.厚环乳牛肝菌(Suillus grevillei)N40与绿木霉(Trichoderma virens)T43复合接种下樟子松苗木的生理响应[J].生态学杂志,2014,33(8):2142-2147.
[20]杨海霞,李士美,李敏,等.丛枝菌根真菌对白三叶耐盐性的影响[J].青岛农业大学学报(自然科学版),2014,31(2):85-90.
[21]杨立宾,宋瑞清,李冲伟.哈茨木霉发酵液乙酸乙酯提取物对致病疫霉生理指标的影响[J].北京林业大学学报,2013,35(2):92-96.
[22]李娟,张耀庭,曾伟,等.应用考马斯亮蓝法测定总蛋自含量[J].中国生物制品学杂志,2000,13(2):118-119.
[23]翁霞,辛广,李云霞.酮比色法测定马铃薯淀粉总糖的条件研究[J].食品研究与开发,2013,34(17):86-88.
[24]李英鹏,孙渭,赵莉丽,等.钾胁迫条件下AM真菌对烟草生长和叶片保护酶系统的影响[J].干旱地区农业研究,2003,21(1):51-54.
[25]沙伟,刘焕婷,谭大海,等.低温胁迫对扎龙芦苇SOD、POD活性和可溶性蛋白含量的影响[J].齐齐哈尔大学学报,2008,24(2):1-4.
[26]冯固,杨茂秋,白灯莎.盐胁迫下VA菌根真菌对无芒雀麦体内矿质元素含量及组成的影响[J].草业学报,1998(3):21-28.
[27] EVELIN H,KAPOOR R,GIRI B.Arbuscular mycorrhizal fungi in alleviation of salt stress:a review[J].Ann Bot,2009,104:1263-1280.
[28]郭绍霞,刘润进.丛枝菌根真菌Glomus mosseae对盐胁迫下牡丹渗透调节的影响[J].植物生理学通讯,2010,46(10):1007-1012.
[29]冯固,李晓林,张福锁,等.施磷和接种AM真菌对玉米耐盐性的影响[J].植物资源与环境学报,2000,9(2):22-26.
[2]耿春女,李培军,陈素华,等.菌根生物修复技术在沈抚污水灌区的应用前景[J].环境污染治理技术与设备,2002,3(7):51-55.
[3] RUIZ-LOZANO J M,AZC魷N R,G魷MEZ M.Alleviation of salt stress by arbuscular-mycorrhizal Glomus species in Lactuca sativa plants[J].Physiologia Plantarum,1996,98:767-772.
[4]冯固,白灯莎,杨茂秋,等.盐胁迫对菌根形成的影响及接种VA菌根真菌对植物生长的效应[J].应用生态学报,1999,10(1):79-82.
[5]盛敏,唐明,张峰峰,等.盐胁迫下接种AM真菌对玉米耐盐性的影响[J].西北植物学报,2011,31(2):332-337.
[6]贺学礼,赵丽莉,李英鹏.NaCl胁迫下AM真菌对棉花生长和叶片保护酶系统的影响[J].生态学报,2005,25(1):188-193.
[7] MUHSIN T M,ZWIAZEK T J.Colonization with Heleloma crustuliniform increases water conductance and limits shoot sodium uptake in white spruce(Picea glauca)seedlings[J].Plant and Soil,2002,23(8):217-225.
[8] CALVO POLANCO M,ZWIAZEK JJ,VOICU MC.Responses of ectomycorrhizal American elm(Ulmus americana)seedlings to salinity and soil compaction[J].Plant Soil,2008,308(1-2):189-200.
[9] BANDOU E,LEBAILLY F,MULLER F,et al.The ectomycorrhizal fungus Scleroderma bermudense alleviates salt stress in seagrape(Coccoloba uvifera L.)seedlings[J].Mycorrhiza,2006,16(8):559-565.
[10]黄艺,姜学艳,梁振春,等.盐胁迫下外生菌根真菌对油松生长及生理的影响[J].农业环境科学学报,2006,25(6):1475-1480.
[11]黄艺,姜学艳,梁振春,等.外生菌根真菌接种和施磷对油松幼苗抗盐性的影响[J].生态环境,2004,13(4):622-625.
[12]张峰峰.甘肃盐碱土植物菌根真菌资源与耐盐性研究[D].杨凌:西北农林科技大学,2007.
[13]李小利.外生菌根真菌菌株耐盐碱性筛选以及黑松(Pinm thunbergii Pari.)菌根苗合成、耐盐碱性研究[D].南京:南京农业大学,2013.
[14]范克胜,吴小芹,任嘉红,等.盐胁迫下外生菌根真菌与根际有益细菌互作对杨树光合特性的影响[J].西北植物学报,2011,31(6):1216-1222.
[15]刘强,闫伟.过量钠盐、碱性pH值对褐环乳牛肝菌生长的影响[J].内蒙古农业大学学报,2009,30(2):52-56.
[16]刘强.褐环乳牛肝菌不同菌株及其与油松合成的菌根苗对盐碱的适应性[D].呼和浩特:内蒙古农业大学,2009.
[17]宋小双,邓勋,尹大川,等.褐环乳牛肝菌N94与绿木霉T43复合接种下红皮云杉苗木的生理响应[J].吉林农业大学学报,2015,37(1):37-42,46.
[18]尹大川,杨立宾,邓勋,等.绿木霉对樟子松苗木生长指标及生理生化指标的影响[J].北京林业大学学报,2015,37(1):78-83.
[19]尹大川,邓勋,CHET I,等.厚环乳牛肝菌(Suillus grevillei)N40与绿木霉(Trichoderma virens)T43复合接种下樟子松苗木的生理响应[J].生态学杂志,2014,33(8):2142-2147.
[20]杨海霞,李士美,李敏,等.丛枝菌根真菌对白三叶耐盐性的影响[J].青岛农业大学学报(自然科学版),2014,31(2):85-90.
[21]杨立宾,宋瑞清,李冲伟.哈茨木霉发酵液乙酸乙酯提取物对致病疫霉生理指标的影响[J].北京林业大学学报,2013,35(2):92-96.
[22]李娟,张耀庭,曾伟,等.应用考马斯亮蓝法测定总蛋自含量[J].中国生物制品学杂志,2000,13(2):118-119.
[23]翁霞,辛广,李云霞.酮比色法测定马铃薯淀粉总糖的条件研究[J].食品研究与开发,2013,34(17):86-88.
[24]李英鹏,孙渭,赵莉丽,等.钾胁迫条件下AM真菌对烟草生长和叶片保护酶系统的影响[J].干旱地区农业研究,2003,21(1):51-54.
[25]沙伟,刘焕婷,谭大海,等.低温胁迫对扎龙芦苇SOD、POD活性和可溶性蛋白含量的影响[J].齐齐哈尔大学学报,2008,24(2):1-4.
[26]冯固,杨茂秋,白灯莎.盐胁迫下VA菌根真菌对无芒雀麦体内矿质元素含量及组成的影响[J].草业学报,1998(3):21-28.
[27] EVELIN H,KAPOOR R,GIRI B.Arbuscular mycorrhizal fungi in alleviation of salt stress:a review[J].Ann Bot,2009,104:1263-1280.
[28]郭绍霞,刘润进.丛枝菌根真菌Glomus mosseae对盐胁迫下牡丹渗透调节的影响[J].植物生理学通讯,2010,46(10):1007-1012.
[29]冯固,李晓林,张福锁,等.施磷和接种AM真菌对玉米耐盐性的影响[J].植物资源与环境学报,2000,9(2):22-26.