江苏省6个翘嘴鳜群体的遗传多样性分析
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摘要
利用20对微卫星引物对江苏省5个内陆湖(太湖、滆湖、洪泽湖、高邮湖和骆马湖)和长江的翘嘴鳜(Siniperca chuatsi)野生种群进行遗传多样性分析,研究江苏省不同水域翘嘴鳜的遗传多样性差异和亲缘关系。结果表明,6个水域的翘嘴鳜群体遗传多样性丰富,其中长江翘嘴鳜群体的平均有效等位基因数为6. 524 8,平均期望杂合度为0. 922,平均多态信息含量为0. 827 3,均高于其它5个翘嘴鳜群体,说明长江翘嘴鳜群体的遗传多样性高于其它5湖的翘嘴鳜群体。哈迪温伯格平衡显示,只有2个位点上存在不平衡,其余都满足哈迪温伯格平衡。从6个水域翘嘴鳜群体之间的遗传相似系数和遗传距离来看,太湖翘嘴鳜群体和滆湖翘嘴鳜群体的亲缘关系最近,骆马湖翘嘴鳜群体和太湖翘嘴鳜群体的亲缘关系最远。研究结果可为江苏省翘嘴鳜种质资源的保护、监测和遗传育种提供分子基础资料。
20 pairs of microsatellite primers were used to analyze the genetic diversity of the wild population of Siniperca chuatsi in 5 inland lakes( Taihu Lake,Gehu Lake,Hongze Lake,Gaoyou Lake and Loma Lake) in Jiangsu Province and in the Yangtze River,the genetic diversity differences and phylogenetic relationships of Siniperca chuatsi in different waters were also analyzed. The results showed that the genetic diversity of Siniperca chuatsi populations in the waters was very rich. The average effective allele number of Siniperca chuatsi population in the Yangtze River was 6. 524 8,the average heterozygosity was 0. 922 and the average polymorphism information content was 0. 827 3. The three indexes were higher than the other five species,indicating that the genetic diversity of Siniperca chuatsi population of the Yangtze River was higher than that of other five Siniperca chuatsi populations. Hardy Weinberg equilibrium showed that only two loci were unbalanced,and the others complied with Hardy Weinberg equilibrium. From the genetic similarity coefficient and genetic distance among the 6 populations of Siniperca chuatsi,the relationship between Taihu Lake population and Gehu Lake population was the closest,and the relationship between Taihu Lake population and Luoma Lake polupation was the farthest. The results of this study provide molecular basis for monitoring,protection and genetic breeding of Siniperca chuatsi germplasm resources in Jiangsu Province.
20 pairs of microsatellite primers were used to analyze the genetic diversity of the wild population of Siniperca chuatsi in 5 inland lakes( Taihu Lake,Gehu Lake,Hongze Lake,Gaoyou Lake and Loma Lake) in Jiangsu Province and in the Yangtze River,the genetic diversity differences and phylogenetic relationships of Siniperca chuatsi in different waters were also analyzed. The results showed that the genetic diversity of Siniperca chuatsi populations in the waters was very rich. The average effective allele number of Siniperca chuatsi population in the Yangtze River was 6. 524 8,the average heterozygosity was 0. 922 and the average polymorphism information content was 0. 827 3. The three indexes were higher than the other five species,indicating that the genetic diversity of Siniperca chuatsi population of the Yangtze River was higher than that of other five Siniperca chuatsi populations. Hardy Weinberg equilibrium showed that only two loci were unbalanced,and the others complied with Hardy Weinberg equilibrium. From the genetic similarity coefficient and genetic distance among the 6 populations of Siniperca chuatsi,the relationship between Taihu Lake population and Gehu Lake population was the closest,and the relationship between Taihu Lake population and Luoma Lake polupation was the farthest. The results of this study provide molecular basis for monitoring,protection and genetic breeding of Siniperca chuatsi germplasm resources in Jiangsu Province.
引文
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[2]符争,李婧,单世涛,等.基于水温的鳜与鲢配套催产模式研究[J].水产科技情报,2017,44(6):335-339.FU Z,LI J,SHAN S T,et al.Study on the oxytocin model of mandarin fish(Siniperca chuatsi)and silver carp(Hypophthalmichthys molitrix)based on water temperature[J].Aquatic Science and Technology Information,2017,44(6):335-339.
[3]梁旭方.鳜鱼感官发育与摄食行为模式定向调控技术[J].水利渔业,2005,9(2):113-125.LIANG X F.Sensory development and feeding behavior pattern directional regulation technology of mandarin fish(Siniperca chuatsi)[J].Water Conservancy,2005,9(2):113-125.
[4]苏胜齐,张海琪,何中央,等.翘嘴鳜和斑鳜肌肉营养成分及氨基酸组成比较[J].西南农业大学学报,2006,27(6):898-901.SU S Q,ZHANG H Q,HE Z Y,et al.Acomparative study of the nutrients and amino acid composition of the muscle of Siniperca chuatsi and Siniperca scherzeri[J].Journal of Southwest Agricultural University,2006,27(6):898-901.
[5]梁银铨,崔希群,刘友亮.鳜肌肉生化成份分析和营养品质评价[J].水生生物学报,1998,22(4):386-388.LIANG Y Q,CUI X Q,LIU Y L.Evaluation of nutritive quality and analysis of the nutritive compositions in the muscle of mandarin fish,Siniperca chuatsi[J].Acta Hydrobiologica Sinica,1998,22(4):386-388.
[6]TANG J Z,ZHANG D Y,CHENG J,et al.Comparative analysis of the amino composition and proteomic patterns of the muscle proteins from two teleosts,Siniperca chuatsi L.and Hypophthalmichthys molitrix L[J].Journal of Fisheries of China,2007,31(3):361-368.
[7]周伟东,周勇,刘奕,等.DNA疫苗浸泡免疫预防鳜鱼传染性脾肾坏死病毒[J].湖北农业科学,2017,56(14):2731-2735.ZHOU W D,ZHOU Y,LIU Y,et al.DNAvaccination of immersion immunitation prevention for Siniperca chuatsi spleen and kidney necrosis virus[J].Hubei Agricultural Sciences,2017,56(14):2731-2735.
[8]杨承泰,王卫民,曹玲.鳜鱼养殖中的常见疾病及其防治[J].水利渔业,2008,28(3):104-107.YANG C T,WANG W M,CAO L.Common diseases and prevention of Siniperca chuatsi in breeding[J].Water Conservancy,2008,28(3):104-107.
[9]李明锋.鳜鱼病害研究进展及前景展望[J].现代渔业信息,2011,26(3):14-16.LI M F.Progress and prospect on disease research of mandarin fish[J].Modern Fisheries Information,2011,26(3):14-16.
[10]李国庆.广东与湖南养殖鳜群体遗传多样性研究[D].华南师范大学,2005.LI G Q.The genetic diversity of farmed mandarin fish(Siniperca chuatsi)stocks in Guangdong&Hunan,China[D].South China Normal University,2005.
[11]刘峰,鲁双庆,刘臻,等.三种鳜鱼(Siniperca)生长激素基因内含子多态性的比较研究[J].海洋与湖沼,2009,40(4):470-478.LIU F,LU S Q,LIU Z,et al.The GH gene diversity among three Siniperca fish species[J].Oceanologia et Limnologia Sinica,2009,40(4):470-478.
[12]方展强,陈军,郑文彪,等.鳜野生群体与养殖群体的RAPD分析[J].大连水产学院学报,2005,20(1):16-19.FANG Z Q,CHEN J,ZHENG W B,et al.RAPDanalysis of wild population and cultivated population in Siniperca chuatsi[J].Journal of Dalian Fisheries University,2005,20(1):16-19.
[13]TOTH G,GASPARI Z,JURKA J.Microsatellite in different eukaryotic genomes:survey and analysis[J].Genome Research,2000,10(7):967-981.
[14]GABRIELE D G,GUIDO C,MARIA T M,et al.Isolation of(AC)n-microsatellites in Vitis vinifera L.and analysis of genetic background in grapevines under marker assisted selection[J].Molecular Breeding,2005,15(1):11-20.
[15]RIAZ S,DANGL G S,EDWARDS K J,et al.Amicrosatellite marker based framework linkage map of Vitis vinifera L[J].Theoretical and Applied Genetics,2004,108(5):864-872.
[16]孙效文,张晓锋,赵莹莹,等.水产生物微卫星标记技术研究进展及其应用[J].中国水产科学,2008,15(4):689-703.SUN X W,ZHANG X F,ZHAO Y Y,et al.Development and application of microsatellite markers in aquatic species[J].Journal of Fishery Sciences of China,2008,15(4):689-703.
[17]LEE B Y,LEE W Y,STREELMAN J T,et al.Asecond-generation genetic linkage map of tilapia[J].Genetics,2005,170(1):237-244.
[18]AGGARWAL R A K,DIXITL S P,VERMA N K,et al.Population genetics analysis of Mehsana goat based on microsatellite markers[J].Current Science,2007,92(8):1133-1137.
[19]李莉好,喻达辉,黄桂菊.尼罗罗非鱼、奥利亚罗非鱼及其正、反杂交群体的遗传多样性[J].中国水产科学,2008,15(4):585-592.LI L H,YU D H,HUANG G J.Genetic diversity of Oreochromis niloticus,O.aureus and their reciprocally crossed hybrid stocks[J].Journal of Fishery Sciences of China,2008,15(4):585-592.
[20]周莉,刘静霞,桂建芳.应用微卫星标记对雌核发育银鲫的遗传多样性初探[J].动物学研究,2001,22(4):257-264.ZHOU L,LIU J X,GUI J F.Preliminary investigation on genetic diversity of gynogenetic silver crucian carp(Carassius auratus gibelio Bloch)detected by microsatellite DNA[J].Zoolgical Research,2001,22(4):257-264.
[21]吴旭,严美姣,李忠杰.长江中下游不同地理种群鳜遗传结构研究[J].水生生物学报,2010,34(4):843-850.WU X,YAN M J,LI Z J.Genetic structure of mandarin fish Siniperca chuatsi in different geographic populations along the middle and lower reaches of Yangtze River Basin[J].Acta Hydrobiologica Sinica,2010,34(4):843-850.
[22]MCCONNEL S,HAMILTON L,MORRIS D,et al.Isolation of Salmonid microsatellite loci and their application to the population genetics of Canadian east coast stocks of Altantic salmon[J].Aquaculture,1995,137(1):19-30.
[23]FERGUSON A.Molecular genetics in fisheries:current and future perspectives[J].Reviews in Fish Biology Fisheries,1994,4(3):379-383.
[24]梅秋兰,刘臻,张学文,等.鳜原种群体和养殖群体遗传多样性的微卫星分析[J].基因组学与应用生物学,2010,29(2):266-272.MEI Q L,LIU Z,ZHANG X W,et al.Analysis of genetic diversity between protospecies and culture populations of Siniperca chuatsi based on microsatellite markers[J].Genomics and Applied Biology,2010,29(2):266-272.
[25]鲁双庆,匡刚桥,刘臻,等.高识别力的微卫星标记系统在鳜属(Siniperca)物种鉴定中的应用[J].海洋与湖沼,2007,38(4):379-384.LU S Q,KUANG G Q,LIU Z,et al.Application of high resolution Microsatellite markers to species identification of Siniperca[J].Oceanologia et Limnologia Sinica,2007,38(4):379-384.
[26]鲁双庆,刘臻,肖调义.微卫星标记在鱼类遗传育种研究中的应用[J].水利渔业,2003,23(6):9-11.LU S Q,LIU Z,XIAO T Y.Application of microsatellite markers in fish genetics and breeding research[J].Water Conservancy,2003,23(6):9-11.
[27]鲁双庆,刘红玉,刘少军,等.黄鳝微卫星引物筛选及其在保护遗传学上的应用[J].水产学报,2005,29(5):612-618.LU S Q,LIU H Y,LIU S J,et al.Screening of microsatellite primer and its application to conservation genetics of Monopterus albus[J].Journal of Fisheries of China,2005,29(5):612-618.
[28]侯宁,张研,鲁翠云,等.微卫星DNA标记分析德国镜鲤的遗传潜力[J].遗传,2007,29(12):1509-1518.HOU N,ZHANG Y,LU C Y,et al.Genetic potential analysis of Germany mirror carp(Cyprinus carpio L.)using microsatellite markers[J].Hereditas,2007,29(12):1509-1518.
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