Ethylenediurea(EDU) pretreatment alleviated the adverse effects of elevated O_3 on Populus alba "Berolinensis" in an urban area
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摘要
Ethylenediurea(EDU)has been used as a chemical protectant against ozone(03).However,its protective effect and physiological mechanisms are still uncertain.The present study aimed to investigate the changes of foliar visible injury,physiological characteristics and emission rates of volatile organic compounds(VOCs)in one-year-old Populus alba"Berolinensis"saplings pretreated with EDU and exposed to elevated O_3(EO,120μg/m~3).The results showed that foliar visible injury symptoms under EO were significantly alleviated in plants with EDU application(p<0.05).Under EO,net photosynthetic rate,the maximum photochemical efficiency of PSII and the photochemical efficiency of PSII of plants pretreated with 300 and600 mg/L EDU were similar to unexposed controls and significantly higher compared to EOstressed plants without EDU pretreatment,respectively.Malondialdehyde content was highest in EO without EDU and decreased significantly by 14.9%and 21.3%with 300 and600 mg/L EDU pretreatment,respectively.EDU pretreatment alone increased superoxide dismutase activity by 10-fold in unexposed plants with further increases of 88.4%and 37.5%in EO plants pretreated with 300 and 600 mg/L EDU pretreatment,respectively(p<0.05).Abscisic acid content declined under EO relative to unexposed controls with the effect partially reversed by EDU pretreatments.Similarly,VOCs emission rate declined under EO relative to unexposed plants with a recovery of emission rate observed with 300 and 600 mg/L EDU pretreatment.These findings provided significant evidence that EDU exerted a beneficial effect and protection on the tested plants against 03 stress.
Ethylenediurea(EDU)has been used as a chemical protectant against ozone(03).However,its protective effect and physiological mechanisms are still uncertain.The present study aimed to investigate the changes of foliar visible injury,physiological characteristics and emission rates of volatile organic compounds(VOCs)in one-year-old Populus alba"Berolinensis"saplings pretreated with EDU and exposed to elevated O_3(EO,120μg/m~3).The results showed that foliar visible injury symptoms under EO were significantly alleviated in plants with EDU application(p<0.05).Under EO,net photosynthetic rate,the maximum photochemical efficiency of PSII and the photochemical efficiency of PSII of plants pretreated with 300 and600 mg/L EDU were similar to unexposed controls and significantly higher compared to EOstressed plants without EDU pretreatment,respectively.Malondialdehyde content was highest in EO without EDU and decreased significantly by 14.9%and 21.3%with 300 and600 mg/L EDU pretreatment,respectively.EDU pretreatment alone increased superoxide dismutase activity by 10-fold in unexposed plants with further increases of 88.4%and 37.5%in EO plants pretreated with 300 and 600 mg/L EDU pretreatment,respectively(p<0.05).Abscisic acid content declined under EO relative to unexposed controls with the effect partially reversed by EDU pretreatments.Similarly,VOCs emission rate declined under EO relative to unexposed plants with a recovery of emission rate observed with 300 and 600 mg/L EDU pretreatment.These findings provided significant evidence that EDU exerted a beneficial effect and protection on the tested plants against 03 stress.
Ethylenediurea(EDU)has been used as a chemical protectant against ozone(03).However,its protective effect and physiological mechanisms are still uncertain.The present study aimed to investigate the changes of foliar visible injury,physiological characteristics and emission rates of volatile organic compounds(VOCs)in one-year-old Populus alba"Berolinensis"saplings pretreated with EDU and exposed to elevated O_3(EO,120μg/m~3).The results showed that foliar visible injury symptoms under EO were significantly alleviated in plants with EDU application(p<0.05).Under EO,net photosynthetic rate,the maximum photochemical efficiency of PSII and the photochemical efficiency of PSII of plants pretreated with 300 and600 mg/L EDU were similar to unexposed controls and significantly higher compared to EOstressed plants without EDU pretreatment,respectively.Malondialdehyde content was highest in EO without EDU and decreased significantly by 14.9%and 21.3%with 300 and600 mg/L EDU pretreatment,respectively.EDU pretreatment alone increased superoxide dismutase activity by 10-fold in unexposed plants with further increases of 88.4%and 37.5%in EO plants pretreated with 300 and 600 mg/L EDU pretreatment,respectively(p<0.05).Abscisic acid content declined under EO relative to unexposed controls with the effect partially reversed by EDU pretreatments.Similarly,VOCs emission rate declined under EO relative to unexposed plants with a recovery of emission rate observed with 300 and 600 mg/L EDU pretreatment.These findings provided significant evidence that EDU exerted a beneficial effect and protection on the tested plants against 03 stress.
引文
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Agrawal,S.B.,Agrawal,M.,1999.Low temperature scanning electron microscope studies of stomatal response in snap'bean plants treated with ozone and ethylenediurea.Biotronics 28,45-53.
Ashrafuzzaman,M.,Haque,Z.,Ali,B.,Mathew,B.,Yu,P.,Hochholdinger,F.,et al.,2018.Ethylenediurea(EDU)mitigates the negative effects of ozone in rice:insights into its mode of action.Plant Cell Environ.41,2882-2898.
Barta,C.,Loreto,F.,2006.The relationship between the methylerythritol phosphate pathway leading to emission of volatile isoprenoids and abscisic acid content in leaves.Plant Physiol.141,1676-1683.
Bortier,K.,Dekelver,G.,Temmerman,L.D.,Ceulemans,R.,2001.Stem injection of Populus nigra with EDU to study ozone effects under field conditions.Environ.Pollut.111,199-208.
Calfapietra,C.,Wiberley,A.E.,Falbel,T.G.,Linskey,A.R.,Mugnozza,G.S.,Karnosky,D.F.,et al.,2007.Isoprene synthase expression and protein levels are reduced under elevated 03but not under elevated CO_2(FACE)in field-grown aspen trees.Plant Cell Environ.30,654-661.
Carriero,G.,Emiliani,G.,Giovannelli,A.,Hoshika,Y.,Paoletti,E.,2015.Effects of long-term ambient ozone exposure on biomass and wood traits in poplar treated with ethylenediurea(EDU).Environ.Pollut.206,575-581.
Chen,Y.J.,Wen,M.X.,Sui,J.X.,Yan,Y.Q.,Yuan,W.,Hong,L.,et al.,2018.Ameliorating effects of leaf water extract of three aromatic plant species on ozone-polluted snap bean(Phaseolus vulgaris L.'Jiangjunyoudou').Bull.Environ.Contam.Toxicol.100,849-855.
Contran,N.,Poletti,E.,Manning,W.J.,Tagliaferro,F.,2009.Ozone sensitivity and ethylenediurea protection in ash trees assessed by JIP chlorophyll a fluorescence transient analysis.Photosynthetica 47,68-78.
Danquah,A.,Zelicourt,A.,Colcombet,J.,Hirt,H.,2014.The role of ABA and MAPK signaling pathways in plant abiotic stress responses.Biotechnol.Adv.32,40-52.
Fares,S.,Vargas,R.,Detto,M.,Goldstein,A.H.,Karlik,J.,Paoletti,E.,et al.,2013.Tropospheric ozone reduces carbon assimilation in trees:estimates from analysis of continuous flux measurements.Glob.Chang.Biol.19,24.27-2443.
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Feng,Z.,Jiang,L.,Calatayud,V.,Dai,L.,Paoletti,E.,2018.Intraspecific variation in sensitivity of winter wheat(Triticum aestivum L.)to ambient ozone in northern China as assessed by ethylenediurea(EDU).Environ.Sci.Pollut.Res.25,29208-29218.
Feng,Z.,Shang,B.,Gao,F.,Calatayud,V.,2019.Current ambient and elevated ozone effects on poplar:a global meta-analysis and response relationships.Sci.Total Environ.654,832-840.
Fischer,R.A.,Turner,N.C.,1978.Plant productivity in the arid and semiarid zones.Annu.Rev.Plant Physiol.29,277-317.
Fridovich,I.,1974.Superoxide dismutases.Adv.Enzymol.41,35-97.
Gao,F.,Calatayud,V.,Garcia,B.F.,Reig,A.J.,Feng,Z.Z.,2016.Effects of elevated ozone on physiological,anatomical andultrastructural characteristics of four common urban tree species in China.Ecol.Indic.67,367-379.
Gong,X.,Hong,S.,Jaffe,D.,2018.Ozone in China:spatial distribution and leading meteorological factors controlling 03in 16 Chinese cities.Aerosol Air Qual.Res.18,2287-2300.
Hassan,I.A.,Bell,J.N.B.,Marshall,F.M.,2007.Effects of air filtration on Egyptian clover(Trifolium alexandrinum L.cv.Messkawy)grown in open-top chambers in a rural site in Egypt.Res.J.Biol.Sci.2,395-402.
He,X.,Huang,W.,Chen,W.,Dong,T.,Liu,C.,Cheng,Z.,et al.,2009.Changes of main secondary metabolites in leaves of Ginkgo biloba in response to ozone fumigation.J.Environ.Sci.21,199-203.
Heath,R.L.,Packer,L.,1968.Photoperoxidation in isolated chloroplasts.I.Kinetics and stoichiometry of fatty acid peroxidation.Arch Biochem.Biophys.125,189-198.
Hoshika,Y.,Pecori,F.,Conese,I.,Bardelli,T.,Paoletti,E.,2013.Effects of a three-year exposure to ambient ozone on biomass allocation in poplar using ethylenediurea.Environ.Pollut.180,299-303.
Hu,Y.,Bellaloui,N.,Sun,G.,Tigabu,M.,Wang,J.,2014.Exogenous sodium sulfide improves morphological and physiological responses of a hybrid Populus species to nitrogen dioxide.J.Plant Physiol.171,868-875.
Huang,J.,Chen,C.,Zhang,W., Ding,C.,Su,X.,Huang,Q.,2017.Effects of drought stress on anatomical structure and photosynthetic characteristics of transgenic JERF36 Populus alba x P.berolinensis seedlings leaves.Sci.Silvae Sine.53,8-15.
Kundu,S., Gantait,S.,2017.Abscisic acid(ABA)signal crosstalk during abiotic stress response.Plant Gene 11,61-69.
Lee,E.H.,Bennett,J.H.,1982.Superoxide dismutase:a possible protective enzyme against O_3 injury in snap beans(Phaseolus vulgaris L.).Plant Physiol.69,1444-1449.
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