渭河流域水体细菌群落的环境响应及生态功能预测
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摘要
流域水体中细菌群落多样性及其代谢功能研究对流域水污染整治及生态修复和水体健康评价具有重要价值.基于Illumina MiSeq高通量测序技术研究渭河流域陕西境内综合治理后水体中细菌群落分布特性,利用冗余分析(redundancy analysis,RDA)方法分析了水体细菌群落与水环境因子的相关关系,并采用PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved states)方法预测和评价了渭河流域细菌群落生态功能.研究结果显示,变形菌门、放线细菌门、蓝藻门和拟杆菌门细菌是水体细菌群落中的主要菌门,占整个细菌群落的相对丰度高达85%,这类细菌与水体中的TP、NO_2~--N、NO_3~--N和TN呈显著的正相关关系(P=0. 02 <0. 05);灞河入渭口下游(S5)细菌群落丰度最高,皂河(S4)细菌群落丰度最低;渭河流域水体致使人类患传染性疾病的可能性较大,且对生物体内分泌系统有较大的影响.本研究成果可为渭河流域水环境健康有序发展提供理论基础.
The diversity of bacterial communities and their metabolic function in the waters of the Weihe River Basin are of great significance for water pollution remediation,ecological restoration,and water quality assessment. Illumina MiSeq high-throughput sequencing technology was applied to study the bacterial community distribution characteristics after the comprehensive treatment of the Shaanxi part of the Weihe River Basin. Furthermore,the correlation between the bacterial communities and environmental factors was analyzed by redundancy analysis( RDA),and the PICRUSt method was adopted to evaluate the ecological function of the bacterial communities. The results showed that Proteobacteria,Actinobacteria,Cyanobacteria,and Bacteroidetes were the main bacterial communities in the water,accounting for 85% of the total microbial community. Additionally,these bacteria showed a significant positive correlation( P = 0. 02, < 0. 05) with the TP,NO_2~--N,NO_3~--N,and TN. The bacteria community richness of water samples collected in Zaohe River was the lowest of all samples,while which in the downstream of the junction of Bahe river and Weihe river was the highest. In addition,water in the Weihe River Basin had great impact on the endocrine systems of aquatic organisms,and also had a possibility of infectious diseases for humans. Our research provides a theoretical basis for the safe and sustainable development of the water environment in the Weihe River Basin.
The diversity of bacterial communities and their metabolic function in the waters of the Weihe River Basin are of great significance for water pollution remediation,ecological restoration,and water quality assessment. Illumina MiSeq high-throughput sequencing technology was applied to study the bacterial community distribution characteristics after the comprehensive treatment of the Shaanxi part of the Weihe River Basin. Furthermore,the correlation between the bacterial communities and environmental factors was analyzed by redundancy analysis( RDA),and the PICRUSt method was adopted to evaluate the ecological function of the bacterial communities. The results showed that Proteobacteria,Actinobacteria,Cyanobacteria,and Bacteroidetes were the main bacterial communities in the water,accounting for 85% of the total microbial community. Additionally,these bacteria showed a significant positive correlation( P = 0. 02, < 0. 05) with the TP,NO_2~--N,NO_3~--N,and TN. The bacteria community richness of water samples collected in Zaohe River was the lowest of all samples,while which in the downstream of the junction of Bahe river and Weihe river was the highest. In addition,water in the Weihe River Basin had great impact on the endocrine systems of aquatic organisms,and also had a possibility of infectious diseases for humans. Our research provides a theoretical basis for the safe and sustainable development of the water environment in the Weihe River Basin.
引文
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[13]陈兆进,丁传雨,朱静亚,等.丹江口水库枯水期浮游细菌群落组成及影响因素研究[J].中国环境科学,2017,37(1):336-344.Cheng Z J,Ding C Y,Zhu J Y,et al.Community structure and influencing factors of bacterioplankton during low water periods in Danjiangkou reservoir[J].China Environmental Science,2017,37(1):336-344.
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[15]张灿,刘文君,张明露,等.水中细菌内毒素污染特性及检测方法研究进展[J].环境科学,2014,35(4):1597-1601.Zhang C,Liu W J,Zhang M L,et al.Review on characteristics and detecting assay of bacterial endotoxin contamination in water environment[J].Environmental Science,2014,35(4):1597-1601.
[16]Pham T L,Utsumi M.An overview of the accumulation of microcystins in aquatic ecosystems[J].Journal of Environmental Management,2018,213:520-529.
[17]国家环境保护总局.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社,2002.201,211,224,227,279.
[18]Langille M G I,Zaneveld J,Caporaso J G,et al.Predictive functional profiling of microbial communities using 16S rRNAmarker gene sequences[J].Nature Biotechnology,2013,31(9):814-821.
[19]GB 3838-2002,地表水环境质量标准[S].
[20]Tan C,Ma F,Li A,et al.Evaluating the effect of dissolved oxygen on simultaneous nitrification and denitrification in polyurethane foam contact oxidation reactors[J].Water Environment Research,2013,85(3):195-202.
[21]杨浩,张国珍,杨晓妮,等.16S rRNA高通量测序研究集雨窖水中微生物群落结构及多样性[J].环境科学,2018,38(4):1704-1716.Yang H,Zhang G Z,Yang X N,et al.Microbial community structure and diversity in cellar water by 16S rRNA highthroughput sequencing[J].Environmental Science,2017,38(4):1704-1716.
[22]Newton R J,Jones S E,Eiler A,et al.A guide to the natural history of freshwater lake bacteria[J].Microbiology and Molecular Biology Reviews,2011,75(1):14-49.
[23]Chen T,Peng T,Feng C P,et al.The feasibility of an up-flow partially aerated biological filter(U-PABF)for nitrogen and CODremoval from domestic wastewater[J].Bioresource Technology,2016,218:307-317.
[24]王俊华,冯贵颖,唐艳,等.放线菌处理皂河水体的应用研究[J].西北农业学报,2007,16(1):82-86.Wang J H,Feng G Y,Tang Y,et al.An experiment on Zaohe water treated by Actonomyces[J].Acta Agriculturae Borealioccidentalis Sinica,2007,16(1):82-86.
[25]陈丙法,冯慕华,尚丽霞,等.聚积蓝藻不同打捞强度下藻源污染物释放特征研究[J].环境科学学报,2016,36(11):4077-4086.Chen B F,Feng M H,Shang L X,et al.The release characteristics of algae-derived pollutants in different salvage intensities after cyanobacteria accumulation[J].Acta Scientiae Circumstantiae,2016,36(11):4077-4086.
[26]Isanta E,Bezerra T,Fernández I,et al.Microbial community shifts on an anammox reactor after a temperature shock using 454-pyrosequencing analysis[J].Bioresource Technology,2015,181:207-213.
[27]Chakravarthy S S,Pande S,Kapoor A,et al.Comparison of denitrification between Paracoccus sp.and Diaphorobacter sp.[J].Applied Biochemistry and Biotechnology,2011,165(1):260-269.
[28]刘芳,杨跃寰.细菌细胞壁肽聚糖的研究[J].四川理工学院学报(自然科学版),2011,24(6):628-631.Liu F,Yang Y H.Research on peptidoglycan of bacterial cell wall[J].Journal of Sichuan University of Science&Engineering(Natural Science Edition),2011,24(6):628-631.
[29]Wang J,Wang Y,Bai J,et al.High efficiency of inorganic nitrogen removal by integrating biofilm-electrode with constructed wetland:autotrophic denitrifying bacteria analysis[J].Bioresource Technology,2017,227:7-14.
[30]张菲,田伟,孙峰,等.丹江口库区表层浮游细菌群落组成与PICRUST功能预测分析[J].环境科学,2019,40(3):1252-1260.Zhang F,Tian W,Sun F,et al.Community structure and predictive functional analysis of surface water bacterioplankton in the Danjiangkou reservoir[J].Environmental Science,2019,40(3):1252-1260.
[31]路璐.深圳湾和大鹏湾微生物群落结构及其功能预测比较分析[J].西昌学院学报(自然科学版),2018,32(2):31-35.Lu L.Comparative analysis on the microbial community structure and function of Shenzheng bay and Dapeng bay[J].Journal of Xichang College(Natural Science Edition),2018,32(2):31-35.
[32]Xu F,Cao F Q,Kong Q,et al.Electricity production and evolution of microbial community in the constructed wetlandmicrobial fuel cell[J].Chemical Engineering Journal,2018,339:479-486.
[2]陕西省人民政府办公厅.陕西省人民政府办公厅关于印发渭河流域水污染防治三年行动方案(2012-2014年)的通知[R].陕西省人民政府公报,2011-12-01.
[3]杜娟,李怀恩,赵湘璧,等.基于SWAT模型的渭河流域污染控制效果模拟[J].生态与农村环境学报,2016,32(4):563-569.Du J,Li H E,Zhao X B,et al.Simulation of pollution control effect in the Weihe River valley with SWAT model[J].Journal of Ecology and Rural Environment,2016,32(4):563-569.
[4]杨学福,关建玲,段晋明,等.渭河西安段水体重金属污染现状及其健康风险评价[J].水土保持通报,2014,34(2):152-156,162.Yang X F,Guan J L,Duan J M,et al.Heavy metal pollution and related health risk of Weihe River in Xi'an section[J].Bulletin of Soil and Water Conservation,2014,34(2):152-156,162.
[5]张成,杨建康.渭河流域陕西段水资源承载力分析[J].水资源与水工程学报,2014,25(5):165-168.Zhang C,Yang J K.Analysis of water resources carrying capacity in Shaanxi section of Weihe River Basin[J].Journal of Water Resources and Water Engineering,2014,25(5):165-168.
[6]马丽丽.淡水生态环境中的浮游微生物检测及群落结构变化研究[D].天津:南开大学,2014.Ma L L.Detection and Community Changes of microbial plankton in freshwater environments[D].Tianjin:Nankai University,2014.
[7]孙寓姣,陈程,丁爱中,等.官厅水库水质特征及水体微生物多样性的响应[J].中国环境科学,2015,35(5):1547-1553.Sun Y J,Chen C,Ding A Z,et al.The corresponding of microbial diversity on water quality and environmental variables of Guanting reservoir[J].China Environmental Science,2015,35(5):1547-1553.
[8]白海锋,赵乃锡,殷旭旺,等.渭河流域浮游动物的群落结构及其与环境因子的关系[J].大连海洋大学学报,2014,29(3):260-266.Bai H F,Zhao N X,Yin X W,et al.Community structure and relationship with environmental factors in zooplankton in Weihe River Basin,Northwest China[J].Journal of Dalian Fisheries University,2014,29(3):260-266.
[9]闵文武,王培培,李丽娟,等.渭河流域浮游植物功能群与环境因子的关系[J].环境科学研究,2015,28(9):1397-1406.Min W W,Wang P P,Li L J,et al.Relationship between phytoplankton functional groups and environmental factors in the Wei River Basin[J].Research of Environmental Sciences,2015,28(9):1397-1406.
[10]殷旭旺,徐宗学,鄢娜,等.渭河流域河流着生藻类的群落结构与生物完整性研究[J].环境科学学报,2013,33(2):518-527.Yin X W,Xu Z X,Yan N,et al.Community structure and biological integrity of periphyton in the Weihe River Basin,China[J].Acta Scientiae Circumstantiae,2013,33(2):518-527.
[11]刘睿,周孝德.水沙环境变化对季节性多沙河流沉积物菌群特征的影响[J].中国环境科学,2017,37(11):4342-4352.Liu R,Zhou X D.Influences of sediment characteristics and overlying water quality on sediment bacterial communities in a seasonal sandy river[J].China Environmental Science,2017,37(11):4342-4352.
[12]程豹,望雪,徐雅倩,等.澜沧江流域浮游细菌群落结构特征及驱动因子分析[J].环境科学,2018,39(8):3649-3659.Cheng B,Wang X,Xu Y Q,et al.Bacterioplankton community structure in the Lancang River basin and the analysis of its driving environmental factors[J].Environmental Science,2018,39(8):3649-3659.
[13]陈兆进,丁传雨,朱静亚,等.丹江口水库枯水期浮游细菌群落组成及影响因素研究[J].中国环境科学,2017,37(1):336-344.Cheng Z J,Ding C Y,Zhu J Y,et al.Community structure and influencing factors of bacterioplankton during low water periods in Danjiangkou reservoir[J].China Environmental Science,2017,37(1):336-344.
[14]薛银刚,刘菲,江晓栋,等.太湖不同湖区冬季沉积物细菌群落多样性[J].中国环境科学,2018,38(2):719-728.Xue Y G,Liu F,Jiang X D,et al.The diversity of bacterial communities in the sediment of different lake zones of lake Taihu in winter[J].China Environmental Science,2018,38(2):719-728.
[15]张灿,刘文君,张明露,等.水中细菌内毒素污染特性及检测方法研究进展[J].环境科学,2014,35(4):1597-1601.Zhang C,Liu W J,Zhang M L,et al.Review on characteristics and detecting assay of bacterial endotoxin contamination in water environment[J].Environmental Science,2014,35(4):1597-1601.
[16]Pham T L,Utsumi M.An overview of the accumulation of microcystins in aquatic ecosystems[J].Journal of Environmental Management,2018,213:520-529.
[17]国家环境保护总局.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社,2002.201,211,224,227,279.
[18]Langille M G I,Zaneveld J,Caporaso J G,et al.Predictive functional profiling of microbial communities using 16S rRNAmarker gene sequences[J].Nature Biotechnology,2013,31(9):814-821.
[19]GB 3838-2002,地表水环境质量标准[S].
[20]Tan C,Ma F,Li A,et al.Evaluating the effect of dissolved oxygen on simultaneous nitrification and denitrification in polyurethane foam contact oxidation reactors[J].Water Environment Research,2013,85(3):195-202.
[21]杨浩,张国珍,杨晓妮,等.16S rRNA高通量测序研究集雨窖水中微生物群落结构及多样性[J].环境科学,2018,38(4):1704-1716.Yang H,Zhang G Z,Yang X N,et al.Microbial community structure and diversity in cellar water by 16S rRNA highthroughput sequencing[J].Environmental Science,2017,38(4):1704-1716.
[22]Newton R J,Jones S E,Eiler A,et al.A guide to the natural history of freshwater lake bacteria[J].Microbiology and Molecular Biology Reviews,2011,75(1):14-49.
[23]Chen T,Peng T,Feng C P,et al.The feasibility of an up-flow partially aerated biological filter(U-PABF)for nitrogen and CODremoval from domestic wastewater[J].Bioresource Technology,2016,218:307-317.
[24]王俊华,冯贵颖,唐艳,等.放线菌处理皂河水体的应用研究[J].西北农业学报,2007,16(1):82-86.Wang J H,Feng G Y,Tang Y,et al.An experiment on Zaohe water treated by Actonomyces[J].Acta Agriculturae Borealioccidentalis Sinica,2007,16(1):82-86.
[25]陈丙法,冯慕华,尚丽霞,等.聚积蓝藻不同打捞强度下藻源污染物释放特征研究[J].环境科学学报,2016,36(11):4077-4086.Chen B F,Feng M H,Shang L X,et al.The release characteristics of algae-derived pollutants in different salvage intensities after cyanobacteria accumulation[J].Acta Scientiae Circumstantiae,2016,36(11):4077-4086.
[26]Isanta E,Bezerra T,Fernández I,et al.Microbial community shifts on an anammox reactor after a temperature shock using 454-pyrosequencing analysis[J].Bioresource Technology,2015,181:207-213.
[27]Chakravarthy S S,Pande S,Kapoor A,et al.Comparison of denitrification between Paracoccus sp.and Diaphorobacter sp.[J].Applied Biochemistry and Biotechnology,2011,165(1):260-269.
[28]刘芳,杨跃寰.细菌细胞壁肽聚糖的研究[J].四川理工学院学报(自然科学版),2011,24(6):628-631.Liu F,Yang Y H.Research on peptidoglycan of bacterial cell wall[J].Journal of Sichuan University of Science&Engineering(Natural Science Edition),2011,24(6):628-631.
[29]Wang J,Wang Y,Bai J,et al.High efficiency of inorganic nitrogen removal by integrating biofilm-electrode with constructed wetland:autotrophic denitrifying bacteria analysis[J].Bioresource Technology,2017,227:7-14.
[30]张菲,田伟,孙峰,等.丹江口库区表层浮游细菌群落组成与PICRUST功能预测分析[J].环境科学,2019,40(3):1252-1260.Zhang F,Tian W,Sun F,et al.Community structure and predictive functional analysis of surface water bacterioplankton in the Danjiangkou reservoir[J].Environmental Science,2019,40(3):1252-1260.
[31]路璐.深圳湾和大鹏湾微生物群落结构及其功能预测比较分析[J].西昌学院学报(自然科学版),2018,32(2):31-35.Lu L.Comparative analysis on the microbial community structure and function of Shenzheng bay and Dapeng bay[J].Journal of Xichang College(Natural Science Edition),2018,32(2):31-35.
[32]Xu F,Cao F Q,Kong Q,et al.Electricity production and evolution of microbial community in the constructed wetlandmicrobial fuel cell[J].Chemical Engineering Journal,2018,339:479-486.