山茶属植物叶片δ~(13)C和δ~(15)N与养分含量及光合作用的关系
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摘要
以11个山茶属植物为试材,测定其叶片稳定碳氮同位素比率(δ13C和δ15N)、光合参数(净光合速率、气孔导度、蒸腾速率、胞间CO2浓度、水分利用效率)以及叶片C、N、P和K元素含量等指标,分析叶片δ13C和δ15N与光合参数、养分含量之间的关系。结果表明:11个山茶属植物δ13C值在-26.38‰~-29.44‰之间,其中多齿红山茶显著高于其它10个物种;δ15N在-3.01‰~4.27‰之间,其中以攸县油茶最高,石果红山茶最低;普通油茶净光合速率最高,是其它10个茶属植物种的124%~496%;11个山茶属植物叶片C含量在47.72%~53.07%之间,叶片N、P含量分别以攸县油茶和普通油茶最高,各物种叶片K含量差异不大;叶片δ13C与C和大量元素含量、光合速率均呈负相关关系,其中与N、P含量以及胞间CO2浓度呈极显著负相关,与水分利用效率呈正相关;δ15N与N、P含量显著正相关,与K含量显著负相关,与光合参数呈正相关,与水分利用效率呈负相关。
Using 11 Camellia plants as test materials, stable carbon and nitrogen isotope ratios(δ15N and δ13C), photosynthetic parameters(Pn, Gs, Tr, Ci and WUEi) and contents of C, N, P and K in leaf were determined and the relations among leaf δ13C, δ15N, photosynthetic parameters and nutrients contents were analyzed. The results showed that the δ13C values of 11 Camellia species were between-26.38 ‰ ~-29.44 ‰, of them, the δ13C value of C. polyodonta was significantly higher than those of other 10 species, and δ15N values were between-3.01 ‰ ~ 4.27 ‰, of them, the δ15N value of C. yuhsienensis was the highest while the lowest value was observed in C. lapidea; Net photosynthetic rate of C. oleifera was 124%~496% of those of other 10 species; Leaf carbon content of 11 Camellia species were between 47.72% ~ 53.07%, leaf N, P contents of C. yuhsienensis and C. oleifera were the highest respectively, and K content in leaves showed little difference among the plant species; Leaf δ13C showed a negative correlation with macro-elements, C content and photosynthetic rate, and showed significantly negatively correlated with N, P content and intercellular CO2 concentration, while positively correlated with water use efficiency; δ15N was positively correlated with N, P content significantly and positively correlated with photosynthetic parameters, while significantly negatively correlated with K content and was negatively correlated water use efficiency.
Using 11 Camellia plants as test materials, stable carbon and nitrogen isotope ratios(δ15N and δ13C), photosynthetic parameters(Pn, Gs, Tr, Ci and WUEi) and contents of C, N, P and K in leaf were determined and the relations among leaf δ13C, δ15N, photosynthetic parameters and nutrients contents were analyzed. The results showed that the δ13C values of 11 Camellia species were between-26.38 ‰ ~-29.44 ‰, of them, the δ13C value of C. polyodonta was significantly higher than those of other 10 species, and δ15N values were between-3.01 ‰ ~ 4.27 ‰, of them, the δ15N value of C. yuhsienensis was the highest while the lowest value was observed in C. lapidea; Net photosynthetic rate of C. oleifera was 124%~496% of those of other 10 species; Leaf carbon content of 11 Camellia species were between 47.72% ~ 53.07%, leaf N, P contents of C. yuhsienensis and C. oleifera were the highest respectively, and K content in leaves showed little difference among the plant species; Leaf δ13C showed a negative correlation with macro-elements, C content and photosynthetic rate, and showed significantly negatively correlated with N, P content and intercellular CO2 concentration, while positively correlated with water use efficiency; δ15N was positively correlated with N, P content significantly and positively correlated with photosynthetic parameters, while significantly negatively correlated with K content and was negatively correlated water use efficiency.
引文
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[2]Robinson D,Handley L L,Scrimgeour C M,et al.Using stable isotope natural abundances(δ15N andδ13C)to integrate the stress responses of wild barley(Hordeum spontaneum C.Koch.)genotypes[J].Journal of experimental botany,2000,51(342):41-50.
[3]邱权,李吉跃,王军辉,等.柴达木盆地白刺叶片δ13C与叶片和土壤养分指标的关系[J].西北植物学报,2013,33(11):23 01-2308.
[4]Lucas A C,Klaus W,Benjamin L T.Plantδ15N Correlates with the Transpiration Effi ciency of Nitrogen Acquisition in Tropical Trees[J].Plant Physiology,2009,151(3):1667–1676.
[5]庄瑞林.中国油茶(第2版)[M].北京:中国林业出版社,2012:1-3.
[6]马锦林,张日清,叶航,等.6个油茶物种的光合特性[J].经济林研究,2012,30(4):79-76.
[7]Yuan J,Tan X,Yuan D,et al.Effect of phosphates on the growth,photosynthesis,and P content of oil tea in acidic red soils[J].Journal of Sustainable Forestry,2013,32:594-604.
[8]唐炜.山茶属三个物种及普通油茶三个品种光合作用测定[D].长沙:中南林业科技大学,2010:1-55.
[9]佘诚棋,程鹏,季琳琳,等.油茶光合作用光响应曲线的拟合[J].经济林研究,2012,30(1):118-120.
[10]裴向阳,钟凤娣,张兵,等.广东省三种典型经济林地土壤性状和养分储量研究[J].经济林研究,2014,32(3):42-47.
[11]刘伟,陈世品,陈辉,等.土壤养分与油茶产量与种仁含油率的相关性研究[J].中南林业科技大学学报,2015,35(3):59-63.
[12]韩家懋,王国安,刘东生.C4植物的出现与全球环境变化[J].地学前缘,2002,9(1):233-243.
[13]邹天才,张著林,周洪英,等.贵州山茶属五种野生植物的光合特性研究[J].园艺学报,1994,21(4):366-370.
[14]Farquhar G D,O'Leary M H,Berry J A.On the Relationship between Carbon Isotope Discrimination and the Intercellular Carbon Dioxide Concentration in Leaves[J].Australian Journal of Plant Physiology,1982,9:121-137.
[15]Farquhar G D,Ehleringer J R,Hubick K T.Carbon isotope discrimin ation and photosynthesis[J].Annual Review of Plant Physiology and Plant Molecular Biology,1989,40(1):503-537.
[16]刘晓宏,赵良菊,Gasaw M,等.东非大裂谷埃塞俄比亚段内C3植物叶片δ13C和δ15N及其环境指示意义[J].科学通报,2007,52(2):199-206.
[17]H?gberg P.Tansley Review No.95δ15N natural abundance in soil-plant systems[J].New Phytologist,1997,137(2):179-203.
[18]Pablo L P,Brenton L,David A P,et al.Carbon(δ13C)and nitrogen(δ15N)stable isotope composition in plant and soil in Southern Patagonia’s native forests[J].Global Change Biology,2012,18(1):311-321.
[19]Makoi J H J R,Chimphango S B M,Dakora F D.Photosynthesis,water-use efficiency andδ13C of five cowpea genotypes grown in mixed culture and at different densities with sorghum[J].Photosynthetica,2010,48(1):143-155.
[20]岳玉麒,郭大立,熊燕梅,等.凉水和宝天曼地区乔木叶片氮素再吸收过程中δ15N的变化[J].生态环境学报,2013,22(3):379-386.