尿石素A浸种对玉米种子活力和萌发的影响
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摘要
种子活力的高低直接影响种子萌发、幼苗生长及后期植株的产量和品质。活力低的种子通过浸种(引发)处理可提高种子活力。尿石素A(UA)是一种可重启线粒体自噬和延长秀丽线虫寿命的天然化合物。以老化5 d的郑单958玉米种子(发芽率约为60%)为材料,分别用0、0.5、2.5、5.0、25.0、50.0和100.0μmol/L尿石素A浸种12 h,探究UA浸种对种子活力和萌发的影响。结果发现,用不同浓度的UA浸种均不同程度地促进种子提前萌发,提高发芽率,增幅为3.67%～26.33%,25μmol/L尿石素A浸种效果最好。通过体内、体外实验分析UA对种胚中主要活性氧清除酶活性的影响,发现均显著提高过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的酶活性,降低丙二醛(MDA)含量。用UA浸种可能通过提高体内活性氧清除酶的活性,进而增强种子的发芽能力。
The level of seed vigor directly influences seed germination, seedling growth and the yield and quality of plants. Pre-soaking(or priming) is a simple technique to improve seed vigor. Urolithin A(UA) is a natural compound that reactivates mitochondrial autophagy and prolongs the lifespan in Caenorhabditis elegans. In this study,maize Zhengdan958 seed aged for 5 days(germination rate of about 60%) were soaked with 0.5, 2.5, 5.0, 25.0, 50.0 and 100.0 μmol/L UA for 12 h, respectively, to explore the effect of seed soaking with UA on seed vigor and germination. The results showed that soaking with UA could promote seed germination in advance and increase the germination rate by 3.67%-26.33%, especially the effect of 25 μmol/L UA treatment. Antioxidation tests performed in vitro and in vivo revealed that UA obviously enhanced the activities of peroxidase(POD), catalase(CAT) and superoxide dismutase(SOD), and decreased the malondialdehyde(MDA) levels. In conclusion, the present results suggested that soaking with UA could improve seed germination ability by enhancing the activities of antioxidant enzymes.
The level of seed vigor directly influences seed germination, seedling growth and the yield and quality of plants. Pre-soaking(or priming) is a simple technique to improve seed vigor. Urolithin A(UA) is a natural compound that reactivates mitochondrial autophagy and prolongs the lifespan in Caenorhabditis elegans. In this study,maize Zhengdan958 seed aged for 5 days(germination rate of about 60%) were soaked with 0.5, 2.5, 5.0, 25.0, 50.0 and 100.0 μmol/L UA for 12 h, respectively, to explore the effect of seed soaking with UA on seed vigor and germination. The results showed that soaking with UA could promote seed germination in advance and increase the germination rate by 3.67%-26.33%, especially the effect of 25 μmol/L UA treatment. Antioxidation tests performed in vitro and in vivo revealed that UA obviously enhanced the activities of peroxidase(POD), catalase(CAT) and superoxide dismutase(SOD), and decreased the malondialdehyde(MDA) levels. In conclusion, the present results suggested that soaking with UA could improve seed germination ability by enhancing the activities of antioxidant enzymes.
引文
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[27]Goel A,Goel A K,Sheoran1 I S.Changes in oxidative stress enzymes during artificial ageing in cotton(Gossypium hirsutum L.)seeds[J].Journal of Plant Physiology,2003,160(9):1093-1100.
[28]Yin G,Xin X,Song C,et al.Activity levels and expression of antioxidant enzymes in the ascorbate-glutathione cycle in artificially aged rice seed[J].Plant Physiology and Biochemistry,2014,80:1-9.
[29]Kong L,Huo H,Mao P.Antioxidant response and related gene expression in aged oat seed[J].Frontiers in Plant Science,2015,6:158.
[2]McDonald M B.Seed priming.In:Black M,Bewley J D,(eds).seed technology and its biological basis[M].Sheffield,England:Sheffield Academic Press,2000.
[3]Beckers G J,Conrath U.Priming for stress resistance:from the lab to the field[J].Current Opinion in Plant Biology,2007,10(4):425-431.
[4]Farooq M,Aziz T,Rehman H U,et al.Evaluating surface drying and re-drying for wheat seed priming with polyamines:effects on emergence,early seedling growth and starch metabolism[J].Acta Physiologiae Plantarum,2011,33(5):1707-1713.
[5]Tian Y,Guan B,Zhou D,et al.Responses of seed germination,seedling growth,and seed yield traits to seed pretreatment in maize(Zea mays L.)[J].The Scientific World Journal,2014:834630.
[6]Iqbal M,Ashraf M.Seed treatment with auxins modulates growth and ion partitioning in salt-stressed wheat plants[J].Journal of Integrative Plant Biology,2007,49(7):1003-1015.
[7]Amooaghaie R,Tabatabaie F.Osmopriming-induced salt tolerance during seed germination of alfalfa most likely mediates through H2O2signaling and upregulation of heme oxygenase[J].Protoplasma,2017,254(4):1791-1803.
[8]Farooq S,Hussain M,Jabran K,et al.Osmopriming with CaCl2improves wheat(Triticum aestivum L.)production under water-limited environments[J].Environmental Science and Pollution Research International,2017,24(15):13638-13649.
[9]Adegbuyi E,Cooper S R,Don R.Osmotic priming of some herbage grass seed using polythylene glycol(PEG)[J].Seed Science and Technology,1981,9(3):867-878.
[10]Bialonska D,Kasimsetty S G,Khan S I,et al.Urolithins,intestinal microbial metabolites of pomegranate ellagitannins,exhibit potent antioxidant activity in a cell-based assay[J].Journal of Agricultural and Food Chemistry.2009,57(21):10181-10186.
[11]Ryu D,Mouchiroud L,Andreux P A,et al.Urolithin A induces mitophagy and prolongs lifespan in C.elegans and increases muscle function in rodents[J].Nature Medicine.2016,22(8):879-888.
[12]Wang Y,Qiu Z,Zhou B,et al.In vitro antiproliferative and antioxidant effects of urolithin A,the colonic metabolite of ellagic acid,on hepatocellular carcinomas HepG2 cells[J].Toxicology in Vitro,2015,29(5):1107-1115.
[13]Zhang W,Chen J H,Aguilera-Barrantes I,et al.Urolithin A suppresses the proliferation of endometrial cancer cells by mediating estrogen receptor-α-dependent gene expression[J].Molecular Nutrition and Food Research,2016,60(11):2387-2395.
[14]Guada M,Ganugula R,Vadhanam M,et al.Urolithin A mitigates cisplatin-induced nephrotoxicity by inhibiting renal inflammation and apoptosis in an experimental rat model[J].Journal of Pharmacology and Experimental Therapeutics,2017,363(1):58-65.
[15]Boakye Y D,Groyer L,Heiss E H.An increased autophagic flux contributes to the anti-inflammatory potential of urolithin A in macrophages[J].Biochimica Et Biophysica Acta,2018,1862(1):61-70.
[16]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2004.
[17]王学奎,黄见良.植物生理生化实验原理与技术(第3版)[M].北京:高等教育出版社,2015.
[18]郝再彬,苍晶,徐仲.植物生理学实验[M].哈尔滨:哈尔滨工业大学出版社,2004.
[19]刘家尧,刘新.植物生理学实验教程[M].北京:高等教育出版社,2010.
[20]Wu X L,Liu H Y,Wang W,et al.Proteomic analysis of seed viability in maize[J].Acta Physiologiae Plantarum,2011,33(1):181-191.
[21]Parkhey S,Naithani S C,Keshavkant S.ROS production and lipid catabolism in desiccating Shorea robusta seeds during aging[J].Plant Physiology and Biochemistry,2012,57:261-267.
[22]Ratajczak E,Ma?ecka A,Bagniewska-Zadworna A,et al.The production,localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech(Fagus sylvatica L.)seeds[J].Journal of Plant Physiology,2015,174:147-156.
[23]宋松泉,程红焱,姜孝成,等.种子生物学[M].北京:科学出版社,2008.
[24]Gill S S,Tuteja N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48(12):909-930.
[25]M?ller I M.Plant mitochondria and oxidative stress:electron transport,NADPH turnover,and metabolism of reactive oxygen species[J].Annual Review of Plant Physiology and Plant Molecular Biology,2001,52:561-591.
[26]Apel K,Hirt H.Reactive oxygen species:metabolism,oxidative stress,and signal transduction[J].Annual Review of Plant Biology,2004,55:373-399.
[27]Goel A,Goel A K,Sheoran1 I S.Changes in oxidative stress enzymes during artificial ageing in cotton(Gossypium hirsutum L.)seeds[J].Journal of Plant Physiology,2003,160(9):1093-1100.
[28]Yin G,Xin X,Song C,et al.Activity levels and expression of antioxidant enzymes in the ascorbate-glutathione cycle in artificially aged rice seed[J].Plant Physiology and Biochemistry,2014,80:1-9.
[29]Kong L,Huo H,Mao P.Antioxidant response and related gene expression in aged oat seed[J].Frontiers in Plant Science,2015,6:158.