丹参与红花水溶性成分配伍抗大鼠脑缺血再灌注损伤的作用研究
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摘要
目的:探讨丹参与红花水溶性成分配伍对大鼠脑缺血再灌注引起损伤的保护作用。方法:SD大鼠随机分为假手术组、模型组、丹参组、红花组、丹参红花配伍组、尼莫地平组,采用大脑中动脉局灶性栓塞(MCAO)模型,观察丹参与红花水溶性成分配伍后对脑缺血再灌注损伤大鼠的神经功能,脑梗死体积,脑含水量和脑指数,脑组织SOD活力、MDA含量,脑组织中IL-1β、IL-6和TNFα等炎症因子水平,以及尼氏小体数等的影响。结果:丹参水溶性成分、红花水溶性成分能更明显地减轻MCAO模型大鼠的神经功能缺损症状,促进神经功能缺损症状的恢复;显著地缩小脑组织梗死;降低脑含水量和脑指数,减轻脑水肿;显著提高脑组织中SOD活力,降低MDA含量,降低缺血侧脑组织IL-1β、IL-6和TNFα等水平;抑制尼氏小体数的减少。丹参水溶性成分与红花水溶性成分组合配伍后效果更佳,且优于单用,对各项指标均有明显改善和提高。结论:丹参水溶性成分与红花水溶性成分配伍对大鼠脑缺血再灌注引起的损伤具有明显的保护作用。
Objective: To observe the protective effects of compatibility water-soluble components of Miltiorrhiza and Saffloweron cerebral ischemia and reperfusion injury. Methods: The SD ratswere randomly divided into groups: sham-operated group, model group, the group of Miltiorrhiza, the group of Safflower, the group of compatibility of Miltiorrhiza and Safflower, and Nimodipine group. Using the focal middle cerebral artery embolization(MCAO) model,we observed targets including neurological deficit score,infarct volume,brain index,brain water content,activity of SOD,content of MDA, levels of IL-1β,IL-6 and TNF-α of ischemic brain tissue, and quantity of nissl body. Results:Water-soluble components of Miltiorrhiza and Safflower could significantly reduce the symptoms of neurological deficits, promote the recovery symptoms of neurological deficits,narrow infarct volume of brain tissue obviously, reduce brain index and brain water,raise the activity of SOD,reduce the content of MDA,levels of IL-1β,IL-6 and TNF-α of ischemic brain tissue and inhibit reducing or disappearing of nissl body. The effects of the compatibility of water-soluble components of Miltiorrhiza and Safflower was better than single use separately.Conclusions:The compatibility of water-soluble components of Miltiorrhiza and Safflower has significant protective effects on ischemic brain injury.
Objective: To observe the protective effects of compatibility water-soluble components of Miltiorrhiza and Saffloweron cerebral ischemia and reperfusion injury. Methods: The SD ratswere randomly divided into groups: sham-operated group, model group, the group of Miltiorrhiza, the group of Safflower, the group of compatibility of Miltiorrhiza and Safflower, and Nimodipine group. Using the focal middle cerebral artery embolization(MCAO) model,we observed targets including neurological deficit score,infarct volume,brain index,brain water content,activity of SOD,content of MDA, levels of IL-1β,IL-6 and TNF-α of ischemic brain tissue, and quantity of nissl body. Results:Water-soluble components of Miltiorrhiza and Safflower could significantly reduce the symptoms of neurological deficits, promote the recovery symptoms of neurological deficits,narrow infarct volume of brain tissue obviously, reduce brain index and brain water,raise the activity of SOD,reduce the content of MDA,levels of IL-1β,IL-6 and TNF-α of ischemic brain tissue and inhibit reducing or disappearing of nissl body. The effects of the compatibility of water-soluble components of Miltiorrhiza and Safflower was better than single use separately.Conclusions:The compatibility of water-soluble components of Miltiorrhiza and Safflower has significant protective effects on ischemic brain injury.
引文
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[15] 刘宏,廖茵茵,张伟健,等.丹红注射液中丹参、红花不同配比与药效的相关性研究[J].中南药学,2018,16(4):438-442.
[16] 乔雪,徐曼,韩健,等.中药复方冠心Ⅱ号的化学成分及药理研究进展[J].世界科学技术-中医药现代化,2007(3):86-95,106.
[17] Yu He,Haitong Wan,Yueguang Du,et al.Protective effect of Danhong injection on cerebral ischemia–reperfusion injury in rats[J].Journal of Ethnopharmacology,2012,144(2):387-394.
[18] 刘剑刚,张大武,李婕,等.丹参、红花水溶性组分及配伍对大鼠心肌缺血/再灌注损伤作用的实验研究[J].中国中药杂志,2011,36(2):189-194.
[19] 李炳乾,张桂龙,余勇波,等.神经干细胞移植对脑缺血再灌注损伤大鼠脑脊液中炎症因子的影响[J].东南大学学报(医学版),2018,37(3):379-384.
[20] 王瑞芳,李月玲,杨玉梅.中药复方制剂抗缺血性脑损伤作用机制研究现状与进展[J].包头医学院学报,2018,34(5):121-123.
[2] 方蕾,杨美娟,蒋玉凤.丹酚酸B对脑缺血再灌注损伤小鼠血管保护作用[J].中华中医药学刊,2016,34(12):3060-3062.
[3] 刘立.丹参对脑缺血再灌注损伤与血液流变的药理作用[J].健康前沿,2015(10):32.
[4] 马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014(7):663-665.
[5] 施峰,刘焱文.红花的化学成分及药理研究进展[J].时珍国医国药,2006,17(9):1666-1667
[6] 袁靖,陈卫东.红花黄色素在心脑血管系统疾病治疗中的应用进展[J].医学研究生学报,2015,28(5):557-560.
[7] 金鸣,李金荣,蔡亚欣,等.红花水溶性成分抗氧化作用的研究[J].心肺血管病杂志,1998,17(4):277-279.
[8] 扈晓佳,殷莎,袁婷婷.红花的化学成分及其药理活性研究进展[J].药学实践杂志,2013,31(3):161-168.
[9] 黄洁红,曾满红.红花总黄素对脑缺血再灌注损伤保护作用的实验研究[J].齐齐哈尔医学院学报,2012,33(7):848-849.
[10] 王瑞芳,李月玲,杨玉梅.中药复方制剂抗缺血性脑损伤作用机制研究现状与进展[J].包头医学院学报,2018(5):121-123.
[11] Ludmila Belayev,Ofelia FA,Raul B,et al.Middle Cerebral Cortery Occlusion in the rat by intraluminal suture[J].Stroke,1996,27(9):1612-1616.
[12] Longa EZ,Weistein PR,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[13] YU YP,XU QQ,ZHENG MZ,et al.Light transmission measurement of focal ischemic cerebral infarction in mice[J].Journal of Zhejiang Unviersity(Medical Sciences),2002,31(2):91-93.
[14] 高丽娜,崔元璐,延阔,等.丹参红花配伍研究进展[J].中草药,2016,47(4):671-679.
[15] 刘宏,廖茵茵,张伟健,等.丹红注射液中丹参、红花不同配比与药效的相关性研究[J].中南药学,2018,16(4):438-442.
[16] 乔雪,徐曼,韩健,等.中药复方冠心Ⅱ号的化学成分及药理研究进展[J].世界科学技术-中医药现代化,2007(3):86-95,106.
[17] Yu He,Haitong Wan,Yueguang Du,et al.Protective effect of Danhong injection on cerebral ischemia–reperfusion injury in rats[J].Journal of Ethnopharmacology,2012,144(2):387-394.
[18] 刘剑刚,张大武,李婕,等.丹参、红花水溶性组分及配伍对大鼠心肌缺血/再灌注损伤作用的实验研究[J].中国中药杂志,2011,36(2):189-194.
[19] 李炳乾,张桂龙,余勇波,等.神经干细胞移植对脑缺血再灌注损伤大鼠脑脊液中炎症因子的影响[J].东南大学学报(医学版),2018,37(3):379-384.
[20] 王瑞芳,李月玲,杨玉梅.中药复方制剂抗缺血性脑损伤作用机制研究现状与进展[J].包头医学院学报,2018,34(5):121-123.