不同比例螺旋藻多糖与银杏叶提取物对小鼠抗疲劳作用的研究
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摘要
研究不同比例螺旋藻多糖与银杏叶提取物复合后对小鼠的抗疲劳作用。将螺旋藻多糖(PSP)与银杏叶提取物(GBE)按不同比例复合,以200 mg·kg~(-1)·d~(-1)剂量分别灌服小鼠,给药14 d后造小鼠疲劳模型。通过检测小鼠力竭游泳时间、血清乳酸、血清尿素氮含量、肝脏肌糖原和肝糖原含量及心脏、肝脏、脾脏和肾脏指数变化,观察复合螺旋藻多糖的抗疲劳效果。结果发现供试药物均可显著延长小鼠的力竭游泳时间,减少运动过程中乳酸和血清尿素氮的产生、加速乳酸的消除,还可增加肌糖原和肝糖原储备。另外,灌服供试药物对小鼠的体重增长、肝脏指数、肾脏指数无显著影响(P>0.05),但可显著提高小鼠的心脏指数和脾脏指数(P<0.01)。结果可知试验的供试药物均可显著提高小鼠的运动耐力,延缓疲劳的产生或加速疲劳的消除,具有一定的抗疲劳作用。PSP与GBE以1∶1和2∶1比例复合使用对提高小鼠的抗疲劳能力能产生较好的协同增效作用。
The aim of this experiment is to study the anti-fatigue effect of different proportions of Spirulina Polysaccharide and Ginkgo Biloba. Polysaccharides from spirulina platensis( PSP) and Ginkgo Biloba extract(GBE) were mixed in different proportions. The compound was treated with intragastric administration of 200 mg·kg-1·d-1 for 14 d,and the mice were treated with fatigue. The exhaustive swimming time,serum lactic acid,serum urea nitrogen content,liver glycogen content and liver glycogen content were detected,heart,liver,spleen,and kidney index changes were examined in this research. Lactic acid and serum urea nitrogen were reduced during exercise. The exhaustive swimming time of mice can be significantly prolonged by the tested drugs,elimination of lactate metabolism was accelerated,and muscle glycogen and glycogen storage were increased by tested drugs. In addition,there was no significant effect on the body weight,liver and kidney index( P>0.05),but the cardiac index and spleen index were significantly improved( P < 0. 01). Studies have shown that all the tested drugs have certain anti fatigue effects,which can significantly improve exercise endurance,delay fatigue or accelerate the elimination of fatigue. The combination of PSP and GBE with 1 ∶ 1 and 2 ∶ 1 ratio is the best synergistic effect to improve the anti-fatigue ability of mice.
The aim of this experiment is to study the anti-fatigue effect of different proportions of Spirulina Polysaccharide and Ginkgo Biloba. Polysaccharides from spirulina platensis( PSP) and Ginkgo Biloba extract(GBE) were mixed in different proportions. The compound was treated with intragastric administration of 200 mg·kg-1·d-1 for 14 d,and the mice were treated with fatigue. The exhaustive swimming time,serum lactic acid,serum urea nitrogen content,liver glycogen content and liver glycogen content were detected,heart,liver,spleen,and kidney index changes were examined in this research. Lactic acid and serum urea nitrogen were reduced during exercise. The exhaustive swimming time of mice can be significantly prolonged by the tested drugs,elimination of lactate metabolism was accelerated,and muscle glycogen and glycogen storage were increased by tested drugs. In addition,there was no significant effect on the body weight,liver and kidney index( P>0.05),but the cardiac index and spleen index were significantly improved( P < 0. 01). Studies have shown that all the tested drugs have certain anti fatigue effects,which can significantly improve exercise endurance,delay fatigue or accelerate the elimination of fatigue. The combination of PSP and GBE with 1 ∶ 1 and 2 ∶ 1 ratio is the best synergistic effect to improve the anti-fatigue ability of mice.
引文
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[14]章梦瑶,付乐,张蕴琨.GBE对运动大鼠中脑SOD活力、MDA含量和DA浓度的影响[J].南京体育学院学报,2015,14(4):54-57.Zhang M Y,Fu L,Zhang Y K.Effects of GBE Motion Midbrain SOD Activity,MDA Concentration and DA Concentrations[J].Joournal of Nanjing Institute of Physical Education:Natural Science,2015,14(4):54-57.
[15]濮海平,刘华,侯琳,等.银杏叶提取物抗鼠脑缺血缺氧的药理学机制研究[J].实用医药杂志,2007,24(10):1226-1228.Pu H P,Liu H,Hou L,et al.NES and S-100mRNA level change in newborn rat brain with hypoxic-ischemic brain damage,and the effects of ginkgo biloba extract on their expressions[J].Practical Journal of Medicine Pharmacy,2007,24(10):1226-1228.
[2]熊斌,王杨科,尚书凤,等.紫色甘薯醇提物抗疲劳机制研究[J].中国食品添加剂,2017(1):65-70.Xiong B,Wang Y K,Shang S F,et al.Study on anti-fatigue mechanism of alcohol extracts of purple sweet potato[J].China Food Addatives,2017(1):65-70.
[3]钱志刚.螺旋藻多糖提取新工艺研究[J].淮海工学院学报,2000,9(2):50-52.Qian Z G.A Study on the New Process to Separate Polysaccharide from Spirulina platensis[J].Journal of Huaihai Institute of Techology,2000,9(2):50-52
[4]盛云峰,宁洪波,李徐延.奶牛瘤胃酸中毒相关血液指标及乳酸含量的测定与分析[J].中国奶牛,2008(6):43-45.Sheng Y F,Ning H B,Li X Y.Determination and Analysis of Correlative Blood Indices and Lactic Acid Content in Dairy Cow Suffered from Ruminant Lactic Acidosis[J].China Dairy Cattle,2008(6):43-45.
[5]方春龙,方春露,魏力深,等.训练方式对水球运动员血清酶和血尿素的影响[J].四川体育科学,2016,35(5):31-34.Fang C L,Fang C L,Wei L S,et al.Influence of Training Models on Water Polo Athletes Blood Serumenzyme and Carbamide Azote[J].Sichuan Sports Science,2016,35(5):31-34.
[6]阴文娅,曾果,张彩云.小鼠肝糖原测定影响因素分析[J].现代预防医学,2004,31(3):359-360.Yin W Y,Zeng G,Zhang Y C.A study on the influence factors of measurement[J].Modern Preventive Medicine,2004,31(3):359-360.
[7]林智君,陈煜森,郑选梅,等.螺旋藻多糖对阿尔茨海默病模型小鼠脑线粒体氧化应激保护[J].海南医学,2016,27(11):1731-1733.Lin Z J,Chen Y S,Zheng X M,et al.Protective effect of spirulina polysaccharide against cerebral mitochondrial oxidative stress in Alzheimer's disease mice model[J].Hainan Med J,2016,27(11):1731-1733.
[8]张雪琳.关于运动性疲劳的氧自由基--脂质过氧化理论概述[J].河北体育学院学报,2000,14(3):67-70.Zhang X L.A review of the theory of lipid peroxidation in oxygen free radicals in exercise induced fatigue[J].Journal of Hebei Institute of Physical Education,2000,14(3):67-70.
[9]王清吉,王友绍,何磊,等.茶多酚和银杏叶有效成分的抗辐射作用研究[J].核技术,2004,27(2):148-150.Wang Q J,Wang Y S,He L,et al.Antiradiation effect of tea polyphenol and effective components from ginkgo leaves[J].Nuclear Technology,2004,27(2):148-150.
[10]于蕾妍,邹本革,周颖,等.螺旋藻复合多糖与银杏提取物对小鼠耐缺氧能力的影响[J].动物医学进展,2017,38(4):72-74.Yu L Y,Zou B G,Zhou Y,et al.Effect of spirulina complex polysaccharide on hypoxia tolerance in mice[J].Progress in Veterinary Medicine,2017,38(4):72-74.
[11]Wu R E,Huang W C,Liao C C,et al.Resveratrol Protects against Physical Fatigue and Improves Exercise Performance in Mice[J].Molecules,2013,18:4689-4702.
[12]宋伟峰,苏俊芳,罗淑媛.荔枝多糖抗疲劳作用的实验研究[J].中药材,2012,35(9):1485-1487.Song W F,Su J F,Luo S Y.Experimental study on Antifatigue Effect of litchi polysaccharide[J].Jorunal of Chinese Medicinal Materials,2012,35(9):1485-1487.
[13]柳富平,赵琅.多巴胺与运动能力的关系[J].辽宁体育科技,2013,35(2):14-17.Liu F P,Zhao L.The relationship between dopamine and exercise ability[J].Liaoning Sport Science and Technology,2013,35(2):14-17.
[14]章梦瑶,付乐,张蕴琨.GBE对运动大鼠中脑SOD活力、MDA含量和DA浓度的影响[J].南京体育学院学报,2015,14(4):54-57.Zhang M Y,Fu L,Zhang Y K.Effects of GBE Motion Midbrain SOD Activity,MDA Concentration and DA Concentrations[J].Joournal of Nanjing Institute of Physical Education:Natural Science,2015,14(4):54-57.
[15]濮海平,刘华,侯琳,等.银杏叶提取物抗鼠脑缺血缺氧的药理学机制研究[J].实用医药杂志,2007,24(10):1226-1228.Pu H P,Liu H,Hou L,et al.NES and S-100mRNA level change in newborn rat brain with hypoxic-ischemic brain damage,and the effects of ginkgo biloba extract on their expressions[J].Practical Journal of Medicine Pharmacy,2007,24(10):1226-1228.