共培养微藻Monoraphidium sp.FXY-10与Chlorella sp.U4341提高油脂产率与沉降率
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摘要
采用共培养技术,在异养条件下培养微藻Chlorella sp.U4341和Monoraphidium sp.FXY-10,提高两株微藻共培养的油脂产率;通过共培养方式促进微藻细胞的自絮凝,加速微藻细胞的沉降,以期降低微藻生物量的采收成本。结果表明:微藻Chlorella sp.U4341与Monoraphidium sp.FXY-10单独培养的油脂产率分别为272.07、268.54 mg/(L·d),而两株微藻共培养的油脂产率提高到315.60 mg/(L·d);两株微藻共培养后,自然沉降5 h,微藻细胞沉降率高于90%,远高于单独培养条件下的沉降率。因此,微藻的共培养技术有望成为解决微藻油脂产率低、采收成本高两大瓶颈问题的解决方案。
The co-cultivation of Chlorella sp. U4341 and Monoraphidium sp. FXY-10 under heterotrophic condition to improve the lipid productivity was studied. The flocculation and sedimentation of microalgae cells were promoted and accelerated by co-cultivation so as to reduce the harvesting cost of microalgae biomass. The results showed that the co-cultivation of Chlorella sp. U4341 and Monoraphidium sp. FXY-10 significantly increased the lipid productivity( 315. 60 mg/( L·d)) compared with mono-cultivations( 272. 07 mg/( L·d) and 268. 54 mg/( L·d)). After sedimentation for 5 h,the flocculation efficiency of microalgae cells in co-cultivation reached above 90%,far higher than that in mono-cultivation. Therefore,co-cultivation of microalgae might be the solution for the low lipid productivity and high harvesting cost of microalgae.
The co-cultivation of Chlorella sp. U4341 and Monoraphidium sp. FXY-10 under heterotrophic condition to improve the lipid productivity was studied. The flocculation and sedimentation of microalgae cells were promoted and accelerated by co-cultivation so as to reduce the harvesting cost of microalgae biomass. The results showed that the co-cultivation of Chlorella sp. U4341 and Monoraphidium sp. FXY-10 significantly increased the lipid productivity( 315. 60 mg/( L·d)) compared with mono-cultivations( 272. 07 mg/( L·d) and 268. 54 mg/( L·d)). After sedimentation for 5 h,the flocculation efficiency of microalgae cells in co-cultivation reached above 90%,far higher than that in mono-cultivation. Therefore,co-cultivation of microalgae might be the solution for the low lipid productivity and high harvesting cost of microalgae.
引文
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[2]YU X,ZHAO P,HE C,et al.Isolation of a novel strain of Monoraphidium sp.and characterization of its potential application as biodiesel feedstock[J].Bioresour Technol,2012,121:256-262.
[3]CHE R Q,DING K,HUANG L,et al.Enhancing biomass and oil accumulation of Monoraphidium sp.FXY-10 by combined fulvic acid and two-step cultivation[J].J Taiwan Inst Cheme,2016,27:19-34.
[4]SHEEHAN J,DUNAHAY T,BENEMANN J,et al.A look back at the U.S.Department of Energy’s Aquatic Species Program—biodiesel from algae[J].Nat Renew Energy Lab,1998(7):1-4.
[5]ZHAO P,YU X Y,LI J J,et al.Enhancing lipid productivity by co-cultivation of Chlorella sp.U4341 and Monoraphidium sp.FXY-10[J].J Biosci Bioeng,2014,3:72-77.
[6]WU Z C,ZHU Y,HUANG W Y,et al.Evaluation of flocculation induced by p H increase for harvesting microalgae and reuse of flocculated medium[J].Bioresour Technol,2012,110:496-502.
[7]SALIM S,VERMUE M H,WIJFFELS R H.Ratio between autoflocculating and target microalgae affects the energyefficient harvesting by bio-flocculation[J].Bioresour Technol,2012,118:49-55.
[8]BLIGH E G,DYER W J.A rapid method of total lipid extraction and purification[J].Can J Biochem Physiol,1959,37:911-917.
[9]王盛林,赵震宇,刘平怀.10种热带富油微藻生物量、总脂含量及脂肪酸组成分析[J].中国油脂,2017,42(8):93-97.
[10]RAMOS M J,FEMNDEZ C M,CASAS A,et al.Influence of fatty acid composition of raw materials on biodiesel properties[J].Bioresour Technol,2009,100:261-268.
[11]张秋云,吴忠富,罗丹,等.新型海藻酸铬复合物的制备及其催化油酸甲酯化合成生物柴油[J].中国油脂,2017,42(7):101-105.
[12]IMASE M,WATANABE K,AOYAGI H,et al.Construction of an artificial symbiotic community using a Chlorella-symbiont association as a model[J].FEMS Microbiol Ecol,2008,63:273-282.
[13]NGUYEN T L,LEE D J,CHANG J S,et al.Effects of ozone and peroxone on algal separation via dispersed air flotation[J].Colloids Surfaces B Biointerf,2013,105:246-250.
[14]XIA S,ZHANG Z,WANG X.Production and characterization of abioflocculant by Proteus mirabilis TJ-1[J].Bioresour Technol,2008,99:6520-6527.
[15]WANG Y,TANG X X.Interactions between Prorocentrum donghaiense Lu and Scrippsiella trochoidea(Stein)Loeblich III under laboratory culture[J].Harmful Algae,2008,7:65-75.
[16]YAMASAKI Y,SHIKATA T,NUKATA A,et al.Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community[J].Int Soc Microb Ecol,2009,3:808-817.
[17]KEARNS K D,HUNTER M D.Toxin-producing Anabaena flos-aquae induces settling of Chlamydomonas reinhardtii,a competing motile algae[J].Microb Ecol,2001,42:80-86.
[18]NASCIMENTO I A,MARQUES S S I,Cabanelas I T D,et al.Screening microalgae strains for biodiesel production:lipid productivity and estimation of fuel quality based on fatty acids profiles as selective criteria[J].Bioenerg Res,2013,6:1-13.
[19]WANG L L,MA F,QU Y Y,et al.Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6[J].World J Microbiol Biotechnol,2011,27:2559-2565.
[20]PEZZOLESI L,SAMORI C,PISTOCCHI R.Flocculation induced by homogeneous and heterogeneous acid treatments in Desmodesmus communis[J].Algal Res,2015,10:145-151.