加味大黄■虫颗粒对精索静脉曲张大鼠附睾区段结构性“微环境”的干预
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摘要
目的:通过观察实验性精索静脉曲张大鼠附睾区段结构性"微环境"主要构件的改变,探讨加味大黄■虫颗粒对实验性精索静脉曲张大鼠附睾精子发育成熟的保护机制。方法:按Turner方法制作模型组、假手术组、迈之灵组及不同剂量加味大黄■虫颗粒组左精索静脉曲张大鼠模型。造模完成后,模型组及假手术组每日予1次等量生理盐水灌胃;迈之灵组每日予1次迈之灵片等量水溶液灌胃;不同剂量加味大黄■虫颗粒组每日各予1次不同浓度加味大黄■虫颗粒等量水溶液灌胃。给药8周后,检测附睾局部"微环境"主要构件的改变。结果:相较于模型组,迈之灵组和加味大黄■虫颗粒高、中剂量组大鼠附睾精子活率升高(P<0.01)。相较于迈之灵组,加味大黄■虫颗粒高剂量组附睾精子活率和浓度增高明显(P<0.05),附睾SOD活性明显增高(P<0.05)。结论:加味大黄■虫颗粒改善精索静脉曲张的附睾区段"微环境"的物理支撑,提高附睾培育精子成熟"微环境"稳定性,创造精子发育成熟的有利条件。
Objective: To observe the change of microenvironment of epididymis section structure of varicocele rats and explore the protection mechanism of sperm development and maturation of Modified Dahuang Zhechong Pill on epididymis of varicocele rats. Methods: According to Turner method,we established left varicocele rats of model group, normal group, Aescuven forte group, Modified Dahuang Zhechong Pill high, medium and low dose groups. After establishing left varicocele rats, model group and normal group were given equivalent normal saline by gavage once a day. Aescuven forte group was given equivalent aqueous solution of Aescuven forte by gavage once a day. The different doses groups of modified Dahuang Zhechong Pill were given equivalent solution of Modified Dahuang Zhechong Pill. We tested the change of microenvironment of epididymis section structure of varicocele rats after eight feeding. Result:The rats' left epididymal sperm motility in the model group was significantly lower than that of the Modified Dahuang Zhechong group in high and medium dose and Aescuven forte group(P<0.01). The rats' left epididymal sperm motility and concentration and the activity of SOD in the Modified Dahuang Zhechong Pill high dose group was significantly higher than those of Aescuven forte group(P<0.05). Conclusion: The Modified Dahuang Zhechong Pill can effectively improve physics support of microenvironment of epididymis section structure of varicocele, effectively improve the stability of microenvironment to the sperm maturation and create favorable conditions to sperm maturation.
Objective: To observe the change of microenvironment of epididymis section structure of varicocele rats and explore the protection mechanism of sperm development and maturation of Modified Dahuang Zhechong Pill on epididymis of varicocele rats. Methods: According to Turner method,we established left varicocele rats of model group, normal group, Aescuven forte group, Modified Dahuang Zhechong Pill high, medium and low dose groups. After establishing left varicocele rats, model group and normal group were given equivalent normal saline by gavage once a day. Aescuven forte group was given equivalent aqueous solution of Aescuven forte by gavage once a day. The different doses groups of modified Dahuang Zhechong Pill were given equivalent solution of Modified Dahuang Zhechong Pill. We tested the change of microenvironment of epididymis section structure of varicocele rats after eight feeding. Result:The rats' left epididymal sperm motility in the model group was significantly lower than that of the Modified Dahuang Zhechong group in high and medium dose and Aescuven forte group(P<0.01). The rats' left epididymal sperm motility and concentration and the activity of SOD in the Modified Dahuang Zhechong Pill high dose group was significantly higher than those of Aescuven forte group(P<0.05). Conclusion: The Modified Dahuang Zhechong Pill can effectively improve physics support of microenvironment of epididymis section structure of varicocele, effectively improve the stability of microenvironment to the sperm maturation and create favorable conditions to sperm maturation.
引文
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[11] DanielG.CYR.附睾内细胞间的相互作用:结构完整与精子成熟的重要决定因素[J].中华男科学,2001,7(6):351-357.
[12] 赵豫刚,周吉,张雪军,等.大鼠精索静脉曲张后附睾上皮细胞凋亡及管腔α-1,4-葡糖苷酶、唾液酸含量观察[J].中华男科学杂志,2006,12(7):619-621.
[13] 王权胜,蓝广和,宾彬,等.从“肾虚血瘀”论治精索静脉曲张性不育40例临床观察[J].中国中医基础医学杂志,2014,20(1):124-125,133.
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[15] 孙恒,胡强,金航,等.铁皮石斛化学成分及药理活性研究进展[J].中国实验方剂学杂志,2017,23(11):225-234.
[16] 王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展[J].中华中医药学刊,2015,33(1):206-211.
[17] 谭迎春,陈子江.补肾活血中药对弱精子症大鼠抗氧化作用的研究[J].南京中医药大学学报,2006,22(4):231-233.
[18] 聂克.大黄药理作用研究及思考[J].山东中医药大学学报,2009,33(3):239-240,262.
[19] 黄镇林,杜清华,王宏涛,等.土鳖虫多肽F2-2的体外药效研究[J].中医药信息,2014,31(2):4-6.
[20] 梁进权,宓穗卿,王宁生.水蛭、虻虫配伍的抗凝血和抗血小板聚集的作用[J].中药材,2009,33(9):1347-1350.
[21] 张秋养,邱曙东,马晓年,等.性成熟期大鼠实验性精索静脉曲张对附睾显微和超微结构的影响[J].解剖学报,2003,34(2):182-186.
[2] 黄宇烽.精索静脉曲张与男性不育[J].中华男科学杂志,2010,16(3):195-200.
[3] Turner TT.The study of varicocele through the use of animal models[J].Hum Reprod Update,2001,7(1):78-84.
[4] 世界卫生组织.WHO人类精液及精子-宫颈黏液相互作用实验室检验手册[M].北京:人民卫生出版社,2001:6-28.
[5] 马小茹,王淑秋,刘月霞,等.实验性精索静脉曲张诱导青春期大鼠附睾细胞凋亡的研究[J].中国病理生理杂志,2005,21(6):1220-1224.
[6] Agarwal A,Esteves SC.Varicocele and male infertility:current concepts and future perspectives[J].Asian J Androl,2016,18(2):161-162.
[7] 王权胜,王悦良,唐振宇,等.基于Nrf2/ARE通路的加味大黄虫颗粒对精索静脉曲张模型大鼠的生精效应观察[J].时珍国医国药,2017,28(7):1596-1598.
[8] 王权胜,蒋越,陈豪特,等.加味大黄虫颗粒对精索静脉曲张模型大鼠附睾结构改变的干预作用[J].中华男科学杂志,2018,24(3):241-246.
[9] 尹静,王超,张蜀武,等.强精胶囊对精索静脉曲张大鼠附睾显微结构的影响[J].中国中西医结合外科杂志,2013,19(4):401-404.
[10] 库卓华.人精子在附睾中的成熟[J].中华男科学,2001,7(1):1-9.
[11] DanielG.CYR.附睾内细胞间的相互作用:结构完整与精子成熟的重要决定因素[J].中华男科学,2001,7(6):351-357.
[12] 赵豫刚,周吉,张雪军,等.大鼠精索静脉曲张后附睾上皮细胞凋亡及管腔α-1,4-葡糖苷酶、唾液酸含量观察[J].中华男科学杂志,2006,12(7):619-621.
[13] 王权胜,蓝广和,宾彬,等.从“肾虚血瘀”论治精索静脉曲张性不育40例临床观察[J].中国中医基础医学杂志,2014,20(1):124-125,133.
[14] Abdualkader AM,Merzouk A,Ghawi AM,et al.Some biologicalactivities of Malaysian leech saliva extract[J].IIUM Engin J,2011,12(4):1-9.
[15] 孙恒,胡强,金航,等.铁皮石斛化学成分及药理活性研究进展[J].中国实验方剂学杂志,2017,23(11):225-234.
[16] 王雅芳,李婷,唐正海,等.中药黄芩的化学成分及药理研究进展[J].中华中医药学刊,2015,33(1):206-211.
[17] 谭迎春,陈子江.补肾活血中药对弱精子症大鼠抗氧化作用的研究[J].南京中医药大学学报,2006,22(4):231-233.
[18] 聂克.大黄药理作用研究及思考[J].山东中医药大学学报,2009,33(3):239-240,262.
[19] 黄镇林,杜清华,王宏涛,等.土鳖虫多肽F2-2的体外药效研究[J].中医药信息,2014,31(2):4-6.
[20] 梁进权,宓穗卿,王宁生.水蛭、虻虫配伍的抗凝血和抗血小板聚集的作用[J].中药材,2009,33(9):1347-1350.
[21] 张秋养,邱曙东,马晓年,等.性成熟期大鼠实验性精索静脉曲张对附睾显微和超微结构的影响[J].解剖学报,2003,34(2):182-186.