摘要
以2个山月桂品种薄荷糖(‘Peppermint')和牛眼(‘Bullseye')的组培苗为材料,置于25℃、30℃、35℃、40℃、45℃和50℃6个温度下,处理2个小时后,测定植株的相对电导率、游离脯氨酸、可溶性蛋白和叶绿素含量以及超氧化物歧化酶(SOD)活性,并配以Logistic方程拟合求出半致死温度,得出如下主要结论:山月桂‘Peppermint'的半致死温度为42.6℃,山月桂‘Bullseye'的半致死温度为44.9℃。随着人工胁迫温度的升高,山月桂‘Peppermint'和‘Bullseye'的相对电导率逐渐增高,SOD活性逐渐降低,游离脯氨酸、可溶性蛋白、叶绿素A含量和叶绿素B含量会随着温度的升高先增加后减少。两个品种的生理生化指标的数值上存在显著差异,采用隶属函数法对山月桂‘Peppermint'‘Bullseye'的耐热性作综合评价,山月桂‘Bullseye'耐热性强于山月桂‘Peppermint'。
Using the aseptic seedling of Kalmia latifolia‘Peppermint' and‘Bullseye' as experimental materials,the heat tolerance of K.latifolia and some relative physiological indices were determined and analyzed under the stress conditions of 25℃,30℃,35℃,40℃,45℃ and 50℃.The relative conductivity,Chlorophyll,soluble protein content and free proline content of each plant were measured.Caculated the lethal temperature by Logistic equation,and evaluated the cold-hardiness by the subordinate function.The result showed that the LT_(50) for‘Bullseye' and‘Peppermint' were 42.6℃ and 44.9℃.With temperature increasing,the relative electric conductivity of K.latifolia‘Peppermint' and‘Bullseye' increased and SOD activities were gradually dropped,but Pro,soluble protein content and SOD were increased firstly than decreased.There were significant gender differences in physiological-biochemical indexes of‘Peppermint' and‘Bullseye' plants(P<0.05).Results of the subordinative function analysis showed that‘Bullseye' had much stronger hot-resistance than that of‘Peppermint'.
引文
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