摘要
目的：研究羟基喜树碱(hydroxycamptothecine，简称HCPT)对培养兔晶状体上皮细胞(rabbit lens epithelial cells，RLECs)形态、增殖的影响及其机理，筛选防治后发性白内障的药物。
方法：首先进行RLECs体外培养，在第2、3代RLECs中加入HCPT，分别用光学显微镜和透射电镜观察细胞形态的变化。然后，在第2、3代RLECs中加入不同浓度的HCPT，观察HCPT对细胞增殖的影响。
结果，1 HCPT对RLECs的形态具有显著的影响，透射电镜下主要表现为调亡细胞的形态改变。2 HCPT能抑制细胞的增殖，其抑制作用随药物浓度增加、作用时间延长而增强。
结论：1．HCPT通过凋亡机制引起体外培养RLECs的调亡。2．HCPT能抑制体外培养RLECs的增殖，其抑制作用呈剂量依赖性和时间依赖性。3．HCPT可能成为预防后发性白内障的药物。
[Objective] :To study the effect of hydroxycamptothecine (HCPT) on the morphological changes .proliferation of rabbit lens epithelial cells (RLECs) in vitro and to evaluate the drugs used for posterior capsular opacification( PCO) after cataract extract .
Methods] :1 RLECs were cultured in vitro first and then HCPT was added to the rabbit lens epithelial cells of second or third passage.We observed cells morphological changes with light microscope and electron microscope. . 2 HCPT of different concentrations was added to the rabbit lens epithelial cells of second or third passage. The effect of HCPT on the proliferation was observed.
[Results] :1 HCPT could significantly affect the forms of RLECs. Apoptosis was observed with electron microscope.2 HCPT could inhibit the proliferation of RLECs and the number of cells with apoptosis was increased with the increase of the HCPT concentrations and the longing of time.
(Conclusions] :HCPT can induce apoptosis of rabbit lens epithelial cells cultured in vitro. The number of cells with apoptosis was increased with the increase of the HCPT concentrations and the longing of time.HCPT might be a candidate to prevent the posterior capsular opacification.
引文
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