摘要
目的：探讨电针结合经颅磁刺激对焦虑模型大鼠情绪及认知功能的作用。
方法：将50只SD雄性大鼠随机平均分为5组：模型组、模型+电针组(简称模针组)、模型+磁刺激组(简称模磁组)、模型+磁刺激+电针组(简称磁针组)和空白对照组(简称空白组)共5组。运用连续单一刺激(single prolonged stress, SPS)造模后,采用电针大鼠“百会、长强”穴、大鼠颅骨顶点上方经颅磁刺激、电针结合经颅磁刺激三种不同的治疗方法,每日一次、连续3天,治疗结束后,观察大鼠体重变化,采用高架十字迷宫和旷敞实验评价其情绪反应,运用电生理方法技术检测其认知功能的变化。
结果：1.模型组与空白组相比,模型动物的体重增长明显减小(P<0.05),OT%、OE%值明显下降(P<0.01),垂直移动格数显著减少(P<0.05),P3潜伏期显著延迟(P<0.05),CNV潜伏期均显著延迟(P<0.01),CNV波幅显著降低(P<0.01)。
2.模针组与模型组相比,动物的体重增长有明显增高(P<0.05),OT%值、OE%值、垂直移动格数明显增加(P<0.01),P3潜伏期显著提前(P<0.01),CNV波幅显著增高(P<0.01)；与模磁组、磁针组比较,体重增长明显增高(P<0.01)。
3.模磁组与模型组比较,动物的OT%值、OE%值、探头次数、水平移动格数、垂直移动格数均有明显增加(P<0.05或P<0.01),CNV波幅也显著增高(P<0.01)；与模针组比较,CNV波幅显著增高(P<0.01)。4.磁针组与模型组比较,动物的OT%值、OE%值、探头次数、水平移动格数明显增加(P<0.05),CNV波幅显著增高(P<0.01)；与模针组比较,动物站立次数、水平移动格数、垂直移动格数明显增加(P<0.01)；与模磁组比较,动物的水平移动格数、垂直移动格数明显增加(P<0。05或P<0.01)。
结论：1.电针、经颅磁刺激、电针结合经颅磁刺激三种治疗方法均可不同程度减轻焦虑模型大鼠的情绪反应和改善受损的认知功能。2.单纯电针治疗在保持动物体重增长方面优于单纯经颅磁刺激治疗和电针结合磁刺激治疗；单纯经颅磁刺激治疗在改善认知方面优于单纯电针治疗；电针结合经颅磁刺激治疗在旷敞实验改善焦虑情绪方面优于模针组、模磁组。
Objective The aim of this study was to investigate the effects of electric acupuncture combined with rTMS on emotions and ERPs in rat model of anxiety following SPS stress.
Methods Fifty male rats were randomly divided into 5 groups:the empty group(K)、the model group(M)、the model group accepted electric acupuncture treatment(Z)、the model group accepted both electric acupuncture and rTMS treatment(CZ)、the model group accepted rTMS treatment(C). Rats were subjected to SPS stress, the next day they accepted different treatment in each group, groupZ used electric acupuncture on Baihui point (DU20) and Changqiang point (DU1), groupC used high-frequency (18.3Hz) rTMS, the stimulation consisted of 25 trains of 16 pulses, group CZ used both electric acupuncture and rTMS treatment. Once per day, keep on 3 days. Body weight gain, elevated plus maze test and open field test were performed. After being anesthetized with Chloral Hydrate, P3-like potentials were evoked by the oddball auditory stimulation and CNV-like potentials were elicited by condition-test stimulus.
Results 1. Body weights increased less in groupM than in groupK, increased less in groupC than in groupK, increased less in groupCZ than in groupK, increased less in groupC than in groupZ, increased less in groupCZ than in groupZ.2. OT% was more in groupC than in groupM, detecting head behavioral could be more observed in groupC/groupZ than in groupM, standing behavioral could be more observed in groupCZ than in groupM/groupZ.3. Horizontal movements and vertical movements were both more in groupCZ than in groupM, vertical movements were more in groupZ/groupK.4. P3 latencies is more prolonged in groupM than in groupK/ groupZ.5. GroupK showed shorter CNV latencies than other groups, groupM showed lower CNV amplitudes than other groups, the CNV amplitudes in groupK were higher than those in groupZ/groupCZ, the CNV amplitudes in groupZ were higher than those in groupC.
Conclusion 1. Both electric acupuncture and high-frequency rTMS could reduce emotional reaction and repair the cognition, so dose the electric acupuncture combined with rTMS.2. Compared with groupC/groupCZ, groupZ showed more dominance.
引文
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