摘要
本研究利用来自日本的优质粳稻品种“月之光”和籼稻品种“明恢63”为亲本杂交（月之光×明恢63）构建了含189个家系的F_2群体，建立含127个SSR标记的遗传连锁图谱。采用QTL作图软件WinQTLCartgrapher2.5对F2群体的抽穗期、株高和千粒重等产量相关性状进行了QTL定位分析，定位的QTL信息对抽穗期、株高、千粒重的遗传研究和分子标记辅助育种有重要意义，其主要研究结果如下：
1.选择均匀覆盖整个水稻基因组的SSR引物701对，在两亲本间筛选到167对引物呈多态性，选择带型清晰且在基因组中分布较均匀的标记构建了一个含127个SSR分子标记的连锁图谱，使用MAPMAKER/EXP3.0构建连锁图，重组值用Kosambi函数转换成遗传图距（cM），连锁群总长度为2123.1cM，标记间平均距离为16.7cM，群体标记均匀地分布在12条染色体上。
2.用复合区间定位法对水稻的抽穗期QTL进行定位，共发现4个QTLs，分布于第1、6、8、12条染色体上，表型贡献率为6.4%~48.0%。
3.用复合区间定位法对水稻的株高QTL进行定位，共发现4个QTLs，分布于第1、3、8、12条染色体上，表型贡献率为6.3%~21.1%。
4.用复合区间定位法对水稻的粒重QTL进行定位，共发现3个QTLs，分布于第1、6、8条染色体上，表型贡献率为6.2%~10.3%。
5. QTL研究的最终目的是将研究结果和水稻农艺性状相结合，根据水稻性状的具体特点，采取相应的实施路线，使育种更加快速有效，为水稻分子标记辅助选择育种提供了有价值的遗传信息。
Using a F_2population containing189individiuals derived from a Japonica/Indicacross(YuZhiGuang×MingHui63), A linkage map with127SSR marker was constructed,with which QTLscontrolling heading date,plant height and1000grain weight was mapped by the software Cartgrapher2.5.All the QTL information is useful to rice genetic research and molecular breeding for the traits of headingdate,plant height and1000grain weight.The main results were described as follows:
1. Total701SSR markers were used to screen the polymorphism between two parents(YuZhiGuang×MingHui63), Among167SSR markers with polymorphism,127were finally used toconstruct a genetic linkage map, using MAPMAKER/EXP3.0in a population of189lines. reconstructingvalue with Kosambi function convert from a genetic map distance (cM), It spans2123.1cM in total lengthcovering all12chromosomes, with average distance between markers of16.7cM.
2. Four QTLs for heading date were detected, located on1、6、8and12chromsome, accounting for thephenotypic variation of6.4-48.0%respectively.
3. Meanwhile, Four QTLs relating to plant height were detecteded on1、3、8and12chromsome,explaining6.3%~21.1%of the phenotypic variation respectively.
4. Aswhile, three QTLs were detected on1、6、8chromsome for1000grain weight accounting for6.2%~10.3%of the phenotypic variation respectively.
5. QTL the ultimate purpose of this research is combine study to agronomic traits of rice, according tospecific characteristics of rice traits, take corresponding implementation route, make more quickly andeffectively for rice breeding, molecular maker assisted selection breeding provides valuable geneticinformation..
引文
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