摘要
本研究利用微卫星标记技术,首次对甘肃高山细毛羊优质毛品系153只母羊个体进行了遗传多样性检测和研究,统计了等位基因分布、有效等位基因数、杂合度和多态信息含量,并利用SPSS13.0软件进行了微卫星标记位点与各经济性状的相关性分析。
所选的15个微卫星标记有1个未检测到多态,其余14个均表现出高度多态性。多态性标记在该群体中的平均等位基因数为10个,平均有效等位基因数Ne=7.1,平均杂合度H=0.85,平均多态信息含量PIC=0.83。说明甘肃高山细毛羊优质毛品系拥有丰富的遗传多样性,选择潜力较大,可进一步加强选育。
14个有多态性微卫星座位与经济性状表型数据的关联性分析表明:7个微卫星位点对甘肃高山细毛羊优质毛品系羊毛及体重性状存在显著(p＜0.05)或极显著(p＜0.01)影响。BMS1724与断奶毛长相关显著,BM6506与断奶毛长相关极显著;BL-4、BMS1248与周岁毛长度相关显著,BM6506与周岁毛长度相关极显著;BM6506与周岁腹毛长相关显著;BL-4、BMS1248、BMS1724与周岁污毛量相关显著,BM6506与周岁污毛量相关极显著;BL-4与周岁净毛量相关显著,BMS1724与周岁净毛量和毛纤维细度相关显著,BM6506与周岁净毛量和毛纤维细度相关极显著;URB037与周岁净毛率相关显著,MCM38与周岁净毛率相关极显著;FCB48、MCM38与周岁毛细度变异系数相关显著;BMS1248、BM6506与初生重相关显著;BMS1248、BMS1724与周岁体重相关显著。
通过对有关联的7个微卫星标记进行不同基因型间经济性状的多重比较(Duncan法),找到了以下有利的基因型:在断奶毛长性状上,BM6506位点的186/186为优势基因型,BMS1724位点的156/168、160/176、164/176和164/186为优势基因型;在周岁毛长性状上,BM6506位点的186/186为优势基因型,BMS1248位点的140/160和142/160为优势基因型,BL-4位点的149/169为优势基因型;在周岁腹毛长性状上,BM6506位点的186/186为优势基因型;在周岁污毛量性状上,BMS1248位点的140/160和142/160为优势基因型,BM6506位点的192/192、192/202和194/202为优势基因型,BL-4位点的149/169为优势基因型,BMS1724位点的160/176、164/176、164/186和174/198为优势基因型;在周岁净毛量性状上,BL-4位点的149/169、155/173、155/179和161/179为优势基因型,BM6506位点的192/192、192/202和194/202为优势基因型,BMS1724位点的160/176、164/176、164/186和174/198为优势基因型;在周岁净毛率性状上,MCM38位点的129/151、131/157、135/151、145/163为优势基因型,URB037位点的178/196、196/208、196/212和196/218为优势基因型;在周岁毛纤维细度性状上,BM6506位点的190/190、190/198和198/198为优势基因型,BMS1724位点的178/204为优势基因型;在周岁毛纤维细度变异系数性状上,FCB48位点的135/155、149/159和149/169为优势基因型,MCM38位点的141/157和145/163为优势基因型;在初生重性状上,BMS1248位点的140/160和142/160为优势基因型,BM6506位点的186/186、188/188、192/192和192/202为优势基因型;在周岁体重性状上,BMS1248位点的140/160和142/160为优势基因型,BMS1724位点的160/176、164/176、164/186、174/198和178/204为优势基因型,BM6506位点的186/186、188/188、192/192和192/202为优势基因型。
The genetic diversity of 153 samples in High Quality Fine Wool Strain of Gansu alpine Fine Wool sheep were studied,by using fifteen microsatellite loci.Microsatellite markers-phenotype correlation analysis was made by SPSS13.0.
The results indicated that there had high genetic polymorphism at fourteen loci except one locus among the 15 microsatellite loci.The mean number of alleles was 10, effective number of alleles 7.1,mean heterozygosity 0.85,polymorphisms information content 0.83.It showed that High Quality Fine Wool Strain of Gansu Alpine Fine Wool Sheep had richer genetic diversity and more selection potential,so we can strengthen the process of selection and breeding.
Correlation analysis of those 14 polymorphic Microsatellite loci with economic phenotypes showed that there were significant correlation(P＜0.05) or extreme significant correlation(p＜0.01) among 7 microsatellite loci with wool and weight traits. They are as following:BMS1724 has a significant impact on weaning staple length, BM6506 a extreme significant impact on weaning staple length;BL-4 and BMS1248 a significant impact on staple length at 12 months,BM6506 a extreme significant impact on staple length at 12 months;BM6506 a significant impact on belly staple length at 12 months;BL-4,BMS1248 and BMS 1724 a significant impact on greasy fleece weight at 12 months,BM6506 a extreme significant impact on greasy fleece weight at 12 months; BL-4 a significant impact on clean fleece weight at 12 months,BMS1724 a significant impact on clean fleece weight at 12 months and mean wool fibre diameter at 12 months, BM6506 a extreme significant impact on clean fleece weight at 12 months and mean wool fibre diameter at 12 months;URB037 a significant impact on clean wool yield at 12 months,MCM38 a extreme significant impact on clean wool yield;FCB48 and MCM38 a significant impact on coefficient of variation of wool fibre diameter at 12 months;BMS1248 and BM6506 have a significant impact on body weight at birth; BMS1248 and BMS 1724 have a significant impact on yearling weight.
The multiple comparison was made between genotypes and the markers which have correlations with economic traits.The results are as following:Considering weaning staple length,186/186 is the dominant genotype in the marker BM6506; 156/168,160/176,164/176 and 164/186 are the dominant genotypes in the marker BMS1724.Considering staple length at 12 months,186/186 is the dominant genotype in the marker BM6506;140/160 and 142/160 are the dominant genotypes in the marker BMS1248;149/169 is the dominant genotype in the marker BL-4.Considering belly staple length at 12 months,186/186 is the dominant genotype in the marker BM6506. Considering greasy fleece weigh at 12 months,140/160 and 142/160 are the dominant genotypes in the marker BMS1248;192/192,192/202 and 194/202 in the marker BM6506;149/169 is the dominant genotype in the marker BL-4; 160/176,164/176,164/186 and 174/198 are the dominant genotypes in the marker BMS1724.Considering clean fleece weight at 12 months,149/169,155/173,155/179 and 161/179 are the dominant genotypes in the marker BL-4;192/192,192/202 and 194/202 in the marker BM6506;160/176,164/176,164/186 and 174/198 in the marker BMS1724. Considering clean wool yield at 12 months,129/151,131/157,135/151 and 145/163 are the dominant genotypes in the marker MCM38;178/196,196/208,196/212 and 196/218 in the marker URB037.Considering mean wool fibre diameter at 12 months, 190/190,190/198 and 198/198 are the dominant genotypes in the marker BM6506; 178/204 is the dominant genotype in the marker BMS1724.Considering coefficient of variation of wool fibre diameter at 12 months,135/155,149/159 and 149/169 are the dominant genotypes in the marker FCB48;141/157 and 145/163 in the marker MCM38. Considering body weight at birth,140/160 and 142/160 are the dominant genotypes in the marker BMS1248;186/186,188/188,192/192 and 192/202 in the marker BM6506. Considering yearling weight,140/160 and 142/160 are the dominant genotypes in the marker BMS1248;160/176,164/176,164/186,174/198 and 178/204 in the marker BMS1724;186/186,188/188,192/192 and 192/202 in the marker BM6506.
引文
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