摘要
目的通过对浙江省6家医院收集的原发性痛风患者人群进行中医体质学的调查、分析,探讨痛风人群的中医体质分布规律；利用双向电泳-质谱技术分析痰湿质痛风患者与平和质正常人群的血浆蛋白质的表达差异,研究痰湿体质原发性痛风的差异蛋白特征,从蛋白质组学角度阐释原发性痛风痰湿体质的物质基础。
方法①利用2010年1月至2012年6月,在浙江省6家医院收集的214例样本数据,采用标准化的中医体质量表测评各体质类型的得分；判定每个患者的体质类型。②选取临床诊断8例原发性痰湿质痛风活动期患者；8例平和质正常人；抽取每个人静脉血,分离血浆、制备蛋白质样品,双向电泳,凝胶图像采集、对两组凝胶电泳图谱进行匹配分析,筛选出两者间差异表达蛋白质点数,进行质谱鉴定。
结果①原发性痛风人群中,8种偏颇体质占100%；8种偏颇体质中居于前列的体质类型是痰湿质、阴虚质,分别占21.02%和17.75%；不同的性别、年龄、职业、居住环境与痛风的发病有关。②对两组凝胶电泳图谱进行匹配分析,筛选出两者间差异表达蛋白质点数为26个,选取其中最明显的15个点进行质谱鉴定,成功鉴定出8个蛋白质,其中痰湿质痛风人群与平和质正常人群比较有5个蛋白质(Apolipoprotein C-Ⅱ、 Zinc finger protein562、 Clusterin、Apolipoprotein AI、Transthyretin)表达上调；3个蛋白质(SAPS domain family,member2、LTBP3、Retinol-binding protein4)表达下调。
结论①我们利用《中医体质分类判定标准》及其量表进行对痛风人群进行了横断面调查研究,从而分析原发性痛风的中医体质分布特征及规律,结果发现原发性痛风人群中100%为偏颇体质。②在原发性痛风的发病人群中痰湿质最多占21.02%,其次是阴虚质占17.75%、特禀质占15.42%、气虚质占15.42%；不同的性别、年龄、职业、居住环境与痛风的发病有关；所以原发性痛风的发病是由体质、生活习惯和环境因素的相互作用结果。③我们应用2DE技术比较了痰湿质痛风人群和平和质正常人群的血浆蛋白表达后发现,痰湿质痛风人群有5个蛋白质分子表达量上调,同时有3个蛋白子分子表达量下调。④痰湿质痛风的特异性蛋白质的表达是复杂的,与痰湿体质和炎症因子的表达都有关系。
Objective To describe the gout patients constitution in traditional Chinese medicine distribution, we collected primary gout patients in Zhejiang's6hospitals; We explored the difference protein between phlegm-wetness type gout patients with normal people by using two-dimensional electrophoresis and mass spectrometry.
Methods①214cases data were collected in6hospitals of Zhejiang Province, assessing each patient's constitutional types in Traditional Chinese Medical.②Selected8phlegm-wetness type gout patients and8health people as control; Protein was extracted from blood and two-dimensional electrophoresis, mass spectrometry was used to find the difference protein.
Results①All of the primary gout patients is biased constitution; The incidence of primary gout in phlegm-wetness type accounted for21.02%, followed by Yin deficiency accounted for17.75%. Different gender, age, occupation, living environment has a relationship with gout.②There is5up-regulated protein molecules(Apolipoprotein C-Ⅱ, Zinc finger protein562, Clusterin, Apolipoprotein AI, Transthyretin) and3protein molecular (SAPS domain family, member2, LTBP3, Retinol-binding protein4) cut down, in phlegm-wetness type gout patient.
Conclusion①The " Chinese constitution classification criteria " and its scale was conducted a cross-sectional study on gout patients and found that100%of primary gout crowd for biased constitution.②The incidence of primary gout in phlegm-wetness type accounted for21.02%, followed by Yin deficiency accounted for17.75%; the disease of primary gout is composed of interacting physical, living habits and environmental factors.③There is5up-regulated protein molecules in phlegm-wetness type gout patient, and3protein molecular cut down.④Phlegm-wetness type gout protein has relationship with constitution and inflammatory cytokines.
引文
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