长顺绿壳蛋鸡OCX-32基因变异对蛋壳品质的效应研究
|
摘要
为了进一步了解蛋壳基质蛋白OCX-32基因对鸡蛋壳品质的遗传效应,试验以长顺绿壳蛋鸡为研究对象,在测定蛋壳质量后翅静脉采血提取DNA,采用PCR扩增并结合PCR产物直接测序法对OCX-32基因进行单核苷酸多态性(SNP)筛查及与蛋壳品质的关联分析。结果表明:在长顺绿壳蛋鸡OCX-32基因中检测到2个SNP位点,即位于外显子4的g.22 598 071 C>T错义突变,产生2种基因型(CC和CT),其中C为优势等位基因,其频率为0.900,属于低度多态位点;位于内含子4的g.22 598 730T>A突变,产生3种基因型(TT、TA和AA),T为优势等位基因,其频率为0.650,属于中度多态位点。关联性分析结果显示,g.22 598 071 C>T位点对长顺绿壳蛋鸡蛋壳强度、蛋比重、蛋壳比例和蛋白高度有显著影响(P<0.05),g.22 598 730 T>A位点对长顺绿壳蛋鸡的蛋品质10个指标无显著影响(P>0.05)。说明g.22 598 071 C>T位点可能作为鸡蛋壳品质选择的一个遗传标记,OCX-32基因与长顺绿壳蛋鸡蛋品质存在一定的相关性,可作为发掘长顺绿壳蛋鸡蛋品质的分子育种标记。
引文
[1] MANN,MANN M.The proteome of the calcified layer organic matrix of turkey (Meleagris gallopavo) eggshell[J].Proteome Sci,2013,11(1):40.
[2] MANN K,MANN M.Proteomic analysis of quail calcified eggshell matrix:a comparison to chicken and turkey eggshell proteomes[J].Proteome Sci,2015,13(1):22.
[3] MARIE P,LABAS V,BRIONNE A,et al.Quantitative proteomics provides new insights into chicken eggshell matrix protein functions during the primary events of mineralisation and the active calcification phase[J].J Proteomics,2015,126(3):140-154.
[4] RODRIGUEZ-NAVARRO A B,MARIE P,NYS Y,et al.Amorphous calcium carbonate controls avian eggshell mineralization:A new paradigm for understanding rapid eggshell calcification[J].J Struct Biol,2015,190(3):291-303.
[5] 吴景兰,苏安英,丰艳,等.应用Western印迹杂交技术对大鼠组织cAMP受体蛋白的研究[J].郑州大学学报(医学版),1993(3):274-278.
[6] 刘亚平,马美湖.蛋壳特异性基质蛋白结构与功能特性研究进展[J].中国家禽,2015,37(13):50-56.
[7] 肖俊峰,武书庚,张海军,等.四种壳基质蛋白研究进展[J].中国家禽,2012,34(9):44-47.
[8] KAWASAKI K,WEISS K M.Evolutionary genetics of vertebrate tissue mineralization:the origin and evolution of the secretory calcium-binding phosphoprotein family [J].J Exp Zool B Mol Dev Evol,2010,306(3):295-316.
[9] SOCHA J K,SECHMAN A,MIKA M,et al.Effect of growth hormone on steroid concentrations and mRNA expression of their receptor,and selected egg-specific protein genes in the chicken oviduct during pause in laying induced by fasting[J].Domest Anim Endocrinol,2017,61:1-10.
[10] 杨凌霄,刘长国.鸡蛋壳Ovocleidin-116(OC-116)研究进展[J].中国家禽,2015,37(22):38-40.
[11] 叶幼荣,孙杰,张璐,等.蛋鸡不同产蛋期生殖器官中OC17,OC116和OCX32 mRNA相对定量研究[J].中国兽医学报,2015,35(9):1518-1525.
[12] 杨宁.家禽生产学[M].北京:中国农业出版社,2010.
[13] 李万贵,王单单,潘兰兵,等.鸭CD36基因多态与血清生化指标及肉质的关联分析[J].中国畜牧杂志,2015,51(5):1-5.
[14] 潘兰兵.鸭 MyoD1、SCD1、DRD1 基因变异对肉质和血清生化指标的遗传效应研究[D].贵阳:贵州大学,2014.
[15] 刘亚平,马美湖.蛋壳特异性基质蛋白结构与功能特性研究进展[J].中国家禽,2015,37(13):50-56.
[16] FULTON J E,SOLLER M,LUND A R,et al.Variation in the ovocalyxin-32 gene in commercial egg-laying chickens and its relationship with egg production and egg quality traits[J].Anim Genet,2012,43(Suppl1):102-113.
[17] 李俊营,詹凯,毕善辉,等.安徽省4个地方鸡品种OCX-32基因外显子4的多态性及其与淮南麻黄鸡蛋品质的相关分析[J].西北农林科技大学学报(自然科学版),2014,42(5):1-5.
[18] 夏佳豪.汶上芦花鸡蛋品质及其候选基因遗传效应分析[D].泰安:山东农业大学,2018.
[19] 张丽萍,曾涛,卢立志,等.灵昆鸡视黄醇结合蛋白(CRBP4)和蛋壳基质蛋白(OCX-32)基因多态性与产蛋性状的相关性研究[J].农业生物技术学报,2015,23(10):1343-1349.
[2] MANN K,MANN M.Proteomic analysis of quail calcified eggshell matrix:a comparison to chicken and turkey eggshell proteomes[J].Proteome Sci,2015,13(1):22.
[3] MARIE P,LABAS V,BRIONNE A,et al.Quantitative proteomics provides new insights into chicken eggshell matrix protein functions during the primary events of mineralisation and the active calcification phase[J].J Proteomics,2015,126(3):140-154.
[4] RODRIGUEZ-NAVARRO A B,MARIE P,NYS Y,et al.Amorphous calcium carbonate controls avian eggshell mineralization:A new paradigm for understanding rapid eggshell calcification[J].J Struct Biol,2015,190(3):291-303.
[5] 吴景兰,苏安英,丰艳,等.应用Western印迹杂交技术对大鼠组织cAMP受体蛋白的研究[J].郑州大学学报(医学版),1993(3):274-278.
[6] 刘亚平,马美湖.蛋壳特异性基质蛋白结构与功能特性研究进展[J].中国家禽,2015,37(13):50-56.
[7] 肖俊峰,武书庚,张海军,等.四种壳基质蛋白研究进展[J].中国家禽,2012,34(9):44-47.
[8] KAWASAKI K,WEISS K M.Evolutionary genetics of vertebrate tissue mineralization:the origin and evolution of the secretory calcium-binding phosphoprotein family [J].J Exp Zool B Mol Dev Evol,2010,306(3):295-316.
[9] SOCHA J K,SECHMAN A,MIKA M,et al.Effect of growth hormone on steroid concentrations and mRNA expression of their receptor,and selected egg-specific protein genes in the chicken oviduct during pause in laying induced by fasting[J].Domest Anim Endocrinol,2017,61:1-10.
[10] 杨凌霄,刘长国.鸡蛋壳Ovocleidin-116(OC-116)研究进展[J].中国家禽,2015,37(22):38-40.
[11] 叶幼荣,孙杰,张璐,等.蛋鸡不同产蛋期生殖器官中OC17,OC116和OCX32 mRNA相对定量研究[J].中国兽医学报,2015,35(9):1518-1525.
[12] 杨宁.家禽生产学[M].北京:中国农业出版社,2010.
[13] 李万贵,王单单,潘兰兵,等.鸭CD36基因多态与血清生化指标及肉质的关联分析[J].中国畜牧杂志,2015,51(5):1-5.
[14] 潘兰兵.鸭 MyoD1、SCD1、DRD1 基因变异对肉质和血清生化指标的遗传效应研究[D].贵阳:贵州大学,2014.
[15] 刘亚平,马美湖.蛋壳特异性基质蛋白结构与功能特性研究进展[J].中国家禽,2015,37(13):50-56.
[16] FULTON J E,SOLLER M,LUND A R,et al.Variation in the ovocalyxin-32 gene in commercial egg-laying chickens and its relationship with egg production and egg quality traits[J].Anim Genet,2012,43(Suppl1):102-113.
[17] 李俊营,詹凯,毕善辉,等.安徽省4个地方鸡品种OCX-32基因外显子4的多态性及其与淮南麻黄鸡蛋品质的相关分析[J].西北农林科技大学学报(自然科学版),2014,42(5):1-5.
[18] 夏佳豪.汶上芦花鸡蛋品质及其候选基因遗传效应分析[D].泰安:山东农业大学,2018.
[19] 张丽萍,曾涛,卢立志,等.灵昆鸡视黄醇结合蛋白(CRBP4)和蛋壳基质蛋白(OCX-32)基因多态性与产蛋性状的相关性研究[J].农业生物技术学报,2015,23(10):1343-1349.