亚低温对番茄hta9hta11双突变体光合碳同化相关指标的影响
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摘要
<正>表观遗传机制可调控植物对逆境胁迫的应答,组蛋白变体H2A.Z为重要的表观修饰因子,参与多种非生物胁迫~([1-3]),并且,其基因家族成员较多~([4-5])。hta9hta11双突变体植株具有多效性表型,且H2A.Z基因家族其他成员的表达不能补偿HTA9和HTA11蛋白功能缺失~([6])。番茄(Lycopersicon esculentum Mill.)为重要的设施蔬菜和常用的模式植物,对低温敏感~([7])。亚低温条件下番茄hta9hta11双突变体
Taking day temperature of 25 ℃ and night temperature of 20 ℃ as the control group, variations of photosynthetic carbon assimilation related indexes of wild type and hta9 hta11 double mutant of Lycopersicon esculentum Mill. in treatment group(day temperature of 15 ℃ and night temperature of 10 ℃) were compared. The results show that chlorophyll content in hta9 hta11 double mutant in treatment group is slightly lower than that of wild type; its net photosynthetic rate, contents of sucrose and starch, activities of fructose-1,6-bisphosphate aldolase and fructose-1,6-bisphosphatase, and relative expression levels of TK, GAPDH, SBPase, PRK, RbcL, RCA, FBPase and FBA genes are significantly lower than those of wild type; while its ribulose-1,5-bisphosphate carboxylase/oxygenase activity and relative expression level of RbcS gene are significantly higher than those of wild type. It is suggested that photosynthetic carbon assimilation ability of hta9 hta11 double mutant of L. esculentum is lower than that of wild type under mild hypothermia condition, and histone variant H2 A.Z may play an important role in regulating photosynthetic carbon assimilation pathway of L. esculentum.
Taking day temperature of 25 ℃ and night temperature of 20 ℃ as the control group, variations of photosynthetic carbon assimilation related indexes of wild type and hta9 hta11 double mutant of Lycopersicon esculentum Mill. in treatment group(day temperature of 15 ℃ and night temperature of 10 ℃) were compared. The results show that chlorophyll content in hta9 hta11 double mutant in treatment group is slightly lower than that of wild type; its net photosynthetic rate, contents of sucrose and starch, activities of fructose-1,6-bisphosphate aldolase and fructose-1,6-bisphosphatase, and relative expression levels of TK, GAPDH, SBPase, PRK, RbcL, RCA, FBPase and FBA genes are significantly lower than those of wild type; while its ribulose-1,5-bisphosphate carboxylase/oxygenase activity and relative expression level of RbcS gene are significantly higher than those of wild type. It is suggested that photosynthetic carbon assimilation ability of hta9 hta11 double mutant of L. esculentum is lower than that of wild type under mild hypothermia condition, and histone variant H2 A.Z may play an important role in regulating photosynthetic carbon assimilation pathway of L. esculentum.
引文
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[2] NGUYEN N H,CHEONG J J.H2A.Z-containing nucleosomes are evicted to activate AtMYB44 transcription in response to salt stress[J].Biochemical and Biophysical Research Communications,2018,499(4):1039-1043.
[3] KUMAR S V,WIGGE P A.H2A.Z-containing nucleosomes mediate the thermosensory response in Arabidopsis[J].Cell,2010,140(1):136-147.
[4] YI H,SARDESAI N,FUJINUMA T,et al.Constitutive expression exposes functional redundancy between the Arabidipsis histone H2A gene HTA1 and other H2A gene family members[J].The Plant Cell,2006,18(7):1575-1589.
[5] HU Y,LAI Y.Identification and expression analysis of rice histone genes[J].Plant Physiology Biochemistry,2015,86(2):55-65.
[6] MARCH-DíAZ R,REYES J C.The beauty of being a variant:H2A.Z and the SWR1 complex in plants[J].Molecular Plant,2009,2(4):565-577.
[7] CHEN H,CHEN X,CHEN D,et al.A comparison of the low temperature transcriptomes of two tomato genotypes that differ in freezing tolerance:Solanum lycopersicum and Solanum habrochaites[J].BMC Plant Biology,2015,15(1):132.
[8] 杨学东,田守波,朱为民,等.番茄组蛋白变体H2A.Z基因双突变体的获得及其功能研究[J].园艺学报,2018,45(6):1081-1088.
[9] 刘彧,王琳,杨伊如,等.干旱胁迫下转ClNAC9基因露地菊品种‘纽9717’叶片光合及叶绿素荧光特性的比较[J].植物资源与环境学报,2018,27(3):41-48.
[10] YUAN Y,ZHONG M,SHU S,et al.Effects of exogenous putrescine on leaf anatomy and carbohydrate metabolism in cucumber (Cucumis sativus L.) under salt stress[J].Journal of Plant Growth Regulation,2015,34(3):451-464.
[11] MUSTROPH A,ALBRECHT G.Tolerance of crop plants to oxygen deficiency stress:fermentative activity and photosynthetic capacity of entire seedlings under hypoxia and anoxia[J].Physiologia Plantarum,2003,117(4):508-520.
[12] HOLADAY A S,MARTINDALE W,ALRED R,et al.Changes in activities of enzymes of carbon metabolism in leaves during exposure of plants to low temperature[J].Plant Physiology,1992,98(3):1105-1114.
[13] 李翔,桑勤勤,束胜,等.外源油菜素内酯对弱光下番茄幼苗光合碳同化关键酶及其基因的影响[J].园艺学报,2016,43(10):2012-2020.