三亚临春岭森林公园土壤细菌群落多样性
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摘要
为探明旅游开发以及旅游干扰活动对三亚临春岭森林公园土壤细菌群落丰度和多样性的影响,为热带地区森林资源的保护与合理开发提供依据,研究选取三亚临春岭森林公园为调查对象,沿登山步道垂直方向由近及远选取三个区域,游人干扰区(0～3 m)、景观绿化区(10～30 m)、人工成熟林区(100～150 m),分别提取土壤总DNA并利用Illumina高通量测序技术分析土壤细菌的群落构成和多样性。三个区域表层土壤共获得134 713条序列,OTU总数为1 936个,其中三个样地的共有OUT数目为424个,约占OUT总数的21.9%。三个区域的土壤细菌可分为13个门类,变形菌门(Proteobacteria)在三个区域中均占绝对优势。人工成熟林区土壤微生物多样性最高,而游人干扰区和景观绿化区微生物分布更相近。
Taking Sanya Linchunling Forest Park as example, we studied the effect of tourism development and tourism trampling disturbance on diversity and richness of soil bacteria, in order to offer a foundation for the development of forest tourism in tropic regions. In vertical directions along the mountain trails, the area can be divided into three zones, tourist disturbance area(0～3 m), Landscape green area(10～30 m) and mature artificial forest region(100～150 m). The total soil DNA was extracted and sequenced using Illumina's sequencing technology for soil microbial diversity and richness.In the three zones, totally 134 713 sequences were obtained and divided into 1 936 OTUs, homologous OTU number of the three zones is 424 which is about 21.9 % of total OTUs.The soil bacteria in three zones which belonged to 13 phylums, and Proteobacteria make up absolute predominance in the three zones.Soil bacteria diversity in pristine forest region was far greater than the other two zones and the bacterial species composition of Landscape green area and artificial forest region were more similar.
Taking Sanya Linchunling Forest Park as example, we studied the effect of tourism development and tourism trampling disturbance on diversity and richness of soil bacteria, in order to offer a foundation for the development of forest tourism in tropic regions. In vertical directions along the mountain trails, the area can be divided into three zones, tourist disturbance area(0～3 m), Landscape green area(10～30 m) and mature artificial forest region(100～150 m). The total soil DNA was extracted and sequenced using Illumina's sequencing technology for soil microbial diversity and richness.In the three zones, totally 134 713 sequences were obtained and divided into 1 936 OTUs, homologous OTU number of the three zones is 424 which is about 21.9 % of total OTUs.The soil bacteria in three zones which belonged to 13 phylums, and Proteobacteria make up absolute predominance in the three zones.Soil bacteria diversity in pristine forest region was far greater than the other two zones and the bacterial species composition of Landscape green area and artificial forest region were more similar.
引文
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[21] 朱群华,钟昌琴,谌建宇,等.水源地桉树人工林林区水体潜在风险浅析[J].净水技术,2017,36(8):26-31.
[22] 高璇,屈恋,赖芳,等.窿缘桉叶和果实的挥发性成分分析[J].广西科学,2017,24(4):396-400.
[23] 刘小香,谢龙莲,陈秋波,等.桉树化感作用研究进展[J].热带农业科学,2004(2):54-61.
[2] 李鹏,濮励杰,章锦河.旅游活动对土壤环境影响的国内研究进展[J].地理科学进展,2012,31(8):1097-1105.
[3] 巩劼,陆林.旅游环境影响研究进展与启示[J].自然资源学报,2007,22(4):545-556.
[4] 巩劼,陆林,晋秀龙,等.黄山风景区旅游干扰对植物群落及其土壤性质的影响[J].生态学报,2009,29(5):2239-2251.
[5] 王云,朴正吉,关磊,等.环长白山旅游公路对野生动物的影响[J].生态学杂志,2013,32(2):425-435.
[6] 张桂萍,张峰,茹文明.旅游干扰对历山亚高山草甸植物多样性的影响[J].生态学报,2008(1):407-415.
[7] 谭周进,戴素明,谢桂先,等.旅游踩踏对土壤微生物生物量碳、氮、磷的影响[J].环境科学学报,2006(11):1921-1926.
[8] 汪洪旭.旅游干扰对九宫山景区土壤养分、微生物群落结构及酶活性的影响[J].山东农业大学学报(自然科学版),2014,45(5):735-740.
[9] 程建国,纪昌海,罗燕,等.四川九龙山自然保护区林麝栖息地典型植被土壤微生物群落特征[J].西北林学院学报,2017,32(2):205-211.
[10] 杨黎旭.三亚市虎豹岭森林健身公园植物景观营造探讨[J].热带林业,2010,38(2):38-40.
[11] SHI Q,LI C G,DENG J Y.Assessment of impacts of visitors' activities on vegetation in Zhangjiajie National Forest Park[J].Journal of Forestry Research,2002(2):137-140+165.
[12] 钟林生,柴江豪,谢婷,等.旅游活动对黄石寨景区步道的影响评估[J].地理研究,2008,27(5):1071-1077.
[13] 鲍士旦.土壤农化分析(第3版)[M].北京:中国农业出版社,2000.
[14] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[15] 贺晓凌,宋超,张蕾萍,等.垃圾渗滤液对土壤微生物多样性的影响[J].天津工业大学学报,2017,36(1):36-40.
[16] 李会琳,张静,王岚,等.土地利用方式改变对两种酸性土壤微生物群落结构的影响[J].西华师范大学学报(自然科学版),2017,38(4):373-381.
[17] 刘光荣.旅游干扰对庐山风景区微生物多样性的影响[J].山东农业大学学报(自然科学版),2015,46(2):274-279.
[18] 段青倩,樊文华,吴艳军,等.旅游活动对五台山土壤微生物数量及微生物量碳、氮的影响[J].山西农业科学,2014,42(11):1192-1196.
[19] 杨海君,杨成建,肖启明.旅游活动对张家界国家森林公园土壤酶活性与微生物分布的影响[J].生态学杂志,2007(5):617-621.
[20] 刘洋,曾全超,黄懿梅.基于454高通量测序的黄土高原不同乔木林土壤细菌群落特征[J].中国环境科学,2016,36(11):3487-3494.
[21] 朱群华,钟昌琴,谌建宇,等.水源地桉树人工林林区水体潜在风险浅析[J].净水技术,2017,36(8):26-31.
[22] 高璇,屈恋,赖芳,等.窿缘桉叶和果实的挥发性成分分析[J].广西科学,2017,24(4):396-400.
[23] 刘小香,谢龙莲,陈秋波,等.桉树化感作用研究进展[J].热带农业科学,2004(2):54-61.