Calcareous nannofossil analysis were performed on a ～250 m core (DY03, Fengxian, Shanghai) to reconstruct the paleo-environment conditions. The nannofossils exist only from the core depth of 60.8～2 m, in the Chuansha and Nanhui formations of the Upper Pleistocene, and in the Loutang, Shanghai and Qingpu formations of the Holocene. Calcareous nannofossils occur in grey mud, muddy silt or muddy fine sand, but in a lower diversity; Gephyrocapsa oceanica is the predominant species in the core, while Braarudosphaera bigelowii, Helicosphaera hyalina and Helicosphaera wallichii occasionaly occur in some samples, suggesting a mono-species assemblage.