///To understand better the fundamental environmental changes on Earth’s surface system, detailed analyses on depositional facies and sequences of the Lower Cambrian (Stages 1 and 2) were carried out across the western Hubei Province and northwestern Hunan Province in the Middle Yangtze River region, South China, and 16 types of lithofacies in three main lithological categories of carbonate, chert and fine-grained clastic mudrock were identified. On the other side, from northwestern Hunan to Three Gorges area, western Hubei, bedded cherts commonly intercalated with mudstones, more or less, comprise the basal portions of sequences, which tended to be less voluminous, and finally replaced by mudstones (i.e. at Jiuqunao) toward the platform. Afterwards, the Middle Yangtze River region evolved into a new stage of tectono-sedimentary evolution.