Fine mapping of a day-length-sensitive dwarf gene in rice

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• 作者：Min Wang ; Liping Chen ; Ruizhen Qin ; Haitao Zhang ; Yingrao Shi ; Qinquan Chen ; Sheng Luo ; Fan Wang and Zhijun Cheng
• 刊名：Plant Breeding
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：135
• 期：5
• 页码：580-587
• 全文大小：757K
• ISSN：1439-0523

文摘

Dwarf mutants are valuable and crucial resources for genetic research and crop breeding programme in rice. In this study, we identified a dwarf mutant derive from tissue culture, which exhibited a delayed heading date and dwarfism under long-day growth conditions, suggesting the heading date of dwarf mutant was sensitive to day length. Based on 2000 F₂ mutant-like individuals from the cross of the mutant and a Japonica var. ‘IRAT129’, the dwarf gene was finally narrowed into a 512-kb region near the centromere on chromosome 9. According to the sequence analysis of a delimited region, 21 genes had base alternations either in promoters (15 SNPs) or in coding regions (6 InDels) among 73 annotated genes, and five genes were confirmed sequence alternations resulting from their expression mainly in the vegetative organs. Given to the RNAi plants of the five genes incapable to mimic dwarf and late heading date phenotype, the candidate gene remains to be identified by other genetic or molecular methods. Therefore, all these results give us informative foundation for the day-length-sensitive dwarf gene isolation.