Functional Supramolecular Polymers: A Fluorescent Microfibrous Network in a Supramolecular Hydrogel for High-Contrast Live Cell-Material Imaging in 3D Environments

详细信息    查看全文

• 作者：Shu-Min Hsu ; Fang-Yi Wu ; Hsun Cheng ; Yu-Tang Huang ; Yi-Ru Hsieh ; Dion Tzu-Huan Tseng ; Mei-Yu Yeh ; Shih-Chieh Hung and Hsin-Chieh Lin
• 刊名：Advanced Healthcare Materials
• 出版年：2016
• 出版时间：September 21, 2016
• 年：2016
• 卷：5
• 期：18
• 页码：2406-2412
• 全文大小：2758K
• ISSN：2192-2659

文摘

A new bottom-up strategy based on aromatic peptide amphiphile is developed for a high-contrast visualization of 3D live cell-material imaging—something that has been difficult to achieve previously because of the problems associated with the diffraction of light by the nanosized peptide materials and the aggregation-caused quenching of aggregated π-conjugated fluorophores in the nanostructures. This study reports an example of a novel supramolecular hydrogelator, naphthaleneimide-phenylalanine (NI-Phe), which forms a self-supporting hydrogel displaying a unique microfibrous network and promising aggregation-induced emission characteristics at pH 7.4. The storage modulus of the NI-Phe gel supports the mass of a cell for 3D cell culturing. This work illustrates a new dopant-free supramolecular approach, complementary to well-established doping procedures that should facilitate the development of live cell imaging in 3D scaffolding materials.