Preparation of new amphiphilic macroporous nonwoven polymeric adsorbents aimed for selective removal of low-density lipoprotein from plasma

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• 作者：Xiaodong Hou ; Tao Zhang and Amin Cao
• 关键词：low density lipoprotein ; irradiation ; grafting copolymerization ; nonwoven ; adsorbent
• 刊名：Journal of Biomedical Materials Research Part B: Applied Biomaterials
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：103
• 期：1
• 页码：52-61
• 全文大小：708K
• ISSN：1552-4981

文摘

In the present work, new amphiphilic macroporous polymeric adsorbent (AMPA) membranes for LDL-apheresis were prepared by ⁶⁰Co -ray irradiation-induced grafting copolymerization of polypropylene (PP) nonwoven fabric with acrylic acid, followed by bonding cholesterol through linkers of different length. The new AMPA membranes were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, scanning electron microscope (SEM), and contact angle microscopy. Static adsorption and hemo-perfusion tests show these new adsorbents could efficiently remove LDL from human plasma. Meanwhile, the AMPA displayed good adsorption capacity for triglyceride (TG) as well. The static adsorption performance of the AMPA membranes depends on the length of linker. In addition, a balance between the amount of bonded cholesterol and remaining carboxyl group was found necessary to reach the optimal adsorption performance. The best result was achieved by the AMPA membrane PA₁₅C₆−3, by which 62.8 ± 3.8 μg of LDL-C, 16.5 ± 0.71 μg of HDL-C, 132.4 ± 3.0 μg of TG are removed from human plasma per square centimeter.