Validating the Sensitivity of High-Resolution Melting Analysis for JAK2 V617F Mutation in the Clinical Setting

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• 作者：Chien-Yu Lin ; Cheng-Mao Ho ; Gevorg Tamamyan ; Shu-Fen Yang ; Ching-Tien Peng and Jan-Gowth Chang
• 刊名：Journal of Clinical Laboratory Analysis
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：30
• 期：6
• 页码：838-844
• 全文大小：10928K
• ISSN：1098-2825

文摘

Janus kinase 2 (JAK2) plays an important role in normal hematopoietic growth factor signaling. The detection of the JAK2 V617F mutation (c.1849GNT, GTC → TTC) is crucial for the diagnosis of myeloproliferative neoplasm (MPN) and has become the essential criteria for diagnosis of MPN by the WHO. High-resolution melt (HRM) curve analysis is a nongel-based, closed-tube method, in which PCR amplification and subsequent analysis are sequentially performed in the well, making it more convenient than other scanning methodologies.