Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter

详细信息    查看全文

• 作者：Sarah L. Rouse ; Wlliam J. Hawthorne ; Sebastian Lambert ; Marc L. Morgan ; Stephen A. Hare and Stephen Matthews
• 关键词：amyloid transporter ; bacterial outer membrane ; FapF ; Pseudomonas ; C8E4
• 刊名：Acta Crystallographica Section F
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：72
• 期：12
• 页码：892-896
• 全文大小：580K
• ISSN：1744-3091

文摘

Bacteria often produce extracellular amyloid fibres via a multi-component secretion system. Aggregation-prone, unstructured subunits cross the periplasm and are secreted through the outer membrane, after which they self-assemble. Here, significant progress is presented towards solving the high-resolution crystal structure of the novel amyloid transporter FapF from Pseudomonas, which facilitates the secretion of the amyloid-forming polypeptide FapC across the bacterial outer membrane. This represents the first step towards obtaining structural insight into the products of the Pseudomonasfap operon. Initial attempts at crystallizing full-length and N-terminally truncated constructs by refolding techniques were not successful; however, after preparing FapF^106–430 from the membrane fraction, reproducible crystals were obtained using the sitting-drop method of vapour diffusion. Diffraction data have been processed to 2.5 Å resolution. These crystals belonged to the monoclinic space group C121, with unit-cell parameters a = 143.4, b = 124.6, c = 80.4 Å, α = γ = 90, β = 96.32° and three monomers in the asymmetric unit. It was found that the switch to complete detergent exchange into C8E4 was crucial for forming well diffracting crystals, and it is suggested that this combined with limited proteolysis is a potentially useful protocol for membrane β-barrel protein crystallography. The three-dimensional structure of FapF will provide invaluable information on the mechanistic differences of biogenesis between the curli and Fap functional amyloid systems.