Charge-based separation of particles and cells with similar sizes via the wall-induced electrical lift

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• 作者：Cory Thomas ; Xinyu Lu ; Andrew Todd ; Yash Raval ; Tzuen-Rong Tzeng ; Yongxin Song ; Junsheng Wang ; Dongqing Li and Xiangchun Xuan
• 刊名：ELECTROPHORESIS
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：38
• 期：2
• 页码：320-326
• 全文大小：631K
• ISSN：1522-2683

文摘

The separation of particles and cells in a uniform mixture has been extensively studied as a necessity in many chemical and biomedical engineering and research fields. This work demonstrates a continuous charge-based separation of fluorescent and plain spherical polystyrene particles with comparable sizes in a ψ-shaped microchannel via the wall-induced electrical lift. The effects of both the direct current electric field in the main-branch and the electric field ratio in between the inlet branches for sheath fluid and particle mixture are investigated on this electrokinetic particle separation. A Lagrangian tracking method based theoretical model is also developed to understand the particle transport in the microchannel and simulate the parametric effects on particle separation. Moreover, the demonstrated charge-based separation is applied to a mixture of yeast cells and polystyrene particles with similar sizes. Good separation efficiency and purity are achieved for both the cells and the particles.