Manganese-enhanced MRI (ME MRI) in evaluation of the auditory pathway in an experimental rat model

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• 作者：Zuohua Tang ; Lingjie Wu ; Zebin Xiao ; Xiaoyuan Feng ; Xinghuai Sun ; Weijun Tang ; Jie Wang and Lixin Jin
• 刊名：NMR in Biomedicine
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：30
• 期：2
• 全文大小：561K
• ISSN：1099-1492

文摘

This study aimed to explore the optimal dose and manner of administration for visualization of the auditory pathway on manganese-enhanced MRI (ME MRI). Twenty-four healthy male Sprague–Dawley rats were randomly divided into three experimental groups (n = 8 for Groups A, B and C). The rats in Groups A, B and C were subjected to MnCl₂ injection through the tympanum, inner ear endolymph and perilymph, respectively (0.2 M for four rats and 0.4 M for the others in each group) and observed at 1, 2, 3, 4, 7 and 10 days after the operation with 3.0 T MRI. The signal intensity (SI) and dynamic changes of the auditory pathways at various times, and at two doses through three injection routes, were compared by statistical analysis. Administration of MnCl₂ through the perilymph best showed the complete auditory pathway (P < 0.01), whereas administration though the tympanum only demonstrated part of the pathway. The SI was highest at 24 h after administration of the tracer and began to decline at 48 h. The SI of the auditory cortex was higher after the injection of 0.4 M MnCl₂ than that of 0.2 M MnCl₂. ME MRI best demonstrated the whole auditory pathway at 24 h after the injection of 0.4 M MnCl₂ through the perilymph in the rat, which provided an optimal method for the study of ME MRI of the auditory pathway in the animal model.