Enhancing enzyme hydrolysis of Sorghum stalk by CO₂-pressurized liquid hot water pretreatment

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• 作者：Meng-Jiao Zhao ; Qin-Qin Xu ; Meng-Yuan Zhen ; Guo-Min Li ; Gang-Xu and Jian-Zhong Yin
• 刊名：Environmental Progress & Sustainable Energy
• 出版年：2017
• 出版时间：January, 2017
• 年：2017
• 卷：36
• 期：1
• 页码：208-213
• 全文大小：516K
• ISSN：1944-7450

文摘

CO₂-pressurized liquid hot water was applied to pretreat Sorghum stalk to increase its accessibility for enzyme hydrolysis. Pretreatment temperatures tested ranged from 180 to 220°C, and reaction times varied between 10 and 30 min. The highest total reducing sugar yield of 81.54% was obtained for the sample pretreated at 200°C and 20 min. Different from the N₂-pressurized liquid hot water process, the generated carbonic acid in the CO₂-pressurized method acted as catalyst in the pretreatment process. It was found that the reducing sugar yield increased significantly at lower temperature and shorter reaction time with the addition of CO₂. Finally, X-ray diffraction and scanning electron microscope analysis were used to investigate the microstructure of the Sorghum stalk before and after the pretreatment.