Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment

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• 作者：A. R. von Gundlach ; V. M. Garamus ; T. M. Willey ; J. Ilavsky ; K. Hilpert and A. Rosenhahn
• 关键词：Escherichia coli ultrastructure ; antibiotics ; small-angle X-ray scattering ; SAXS ; ultra-small-angle X-ray scattering ; USAXS ; transmission electron microscopy ; TEM
• 刊名：Journal of Applied Crystallography
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：49
• 期：6
• 页码：2210-2216
• 全文大小：654K
• ISSN：1600-5767

文摘

The application of small-angle X-ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.