In vitro and long-term (2-year follow-up) in vivo osteogenic activities of human periosteum-derived osteoblasts seeded into growth factor-releasing polycaprolactone/pluronic F127 beads scaffolds

详细信息    查看全文

• 作者：Jin Ho Lee ; Dong Kyun Woo ; Tae Ho Kim ; Jun Goo Kang ; Jeong-Won Yun ; Jin-Ho Park ; Bong-Wook Park ; Young-Hoon Kang ; Gyu-Jin Rho ; Si-Jung Jang ; Ji-Sung Park ; Hee-Chun Lee ; Young Min Yoon ; Tae Sung Hwang ; Deok Ryong Kim ; Sun-Chul Hwang ; Dong Hoon Lee ; Ho Young Kim ; Se Heang Oh and June-Ho Byun
• 刊名：Journal of Biomedical Materials Research Part A
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：105
• 期：2
• 页码：363-376
• 全文大小：1183K
• ISSN：1552-4965

文摘

Polycaprolactone (PCL) is a biodegradable polyester that is bioresorbable and biocompatible, and is widely used in medical fields. This study examines in vitro and in vivo osteogenic activities of cultured human periosteum-derived osteoblasts (POs) seeded into growth factor (bone morphogenic protein 2 [BMP-2] or vascular endothelial growth factor [VEGF])-releasing scaffolds of PCL beads coated with Pluronic F127. Each growth factor immobilized in the PCL/F127 is cumulatively released from the beads for more than 40 days (up to 3.04 ± 0.08 ng mg⁻¹ BMP-2 and 3.41 ± 0.18 ng mg⁻¹ VEGF in 42 days). Long-term (∼2 years) experimental results obtained in a miniature pig model suggest that POs seeded into BMP-2 + VEGF-releasing PCL/P-F127 beads are the most effective for bone repair. In in vitro assays, osteogenic activities were higher in POs seeded into BMP-2-releasing PCL/Pluronic F127 beads at earlier time points and in POs seeded into BMP-2 + VEGF-releasing PCL/Pluronic F127 beads at later time points. These results suggest that the combination of BMP-2 and VEGF more sufficiently stimulates (in particular at late time points) osteoblast differentiation of POs seeded in the PCL/F127 in vitro and in vivo, and thus allows effective bone regeneration.