Involvement of viperin in prevention of intrauterine transmission of hepatitis B virus

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• 作者：Xiaoxia Bai ; Huiyan Yang ; Liuxia Wan ; Peng Wang ; Hanzhi Wang ; Xiaofu Yang ; Zhengping Wang and Minyue Dong
• 刊名：APMIS
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：125
• 期：2
• 页码：170-175
• 全文大小：397K
• ISSN：1600-0463

文摘

The aim of this study was to explore the role of viperin in the prevention of intrauterine infection of hepatitis B virus (HBV). Placental samples were collected from seven HBV-positive pregnant women with their infants infected via intrauterine transmission (infected group), 30 HBV-positive women with non-infected infants (non-infected group), and 30 HBV-negative women (controls). The expression of viperin in placenta was analyzed with immunohistochemistry and Western blotting. The expression of viperin of placental trophoblast cell line Swan71 was determined after exposed to HBV. Viperin was localized to syncytiotrophoblast, and there was a significant difference in placental viperin levels among control, non-infected group and infected group (F = 12.824, p < 0.001). The expression of viperin was significantly higher in non-infected group than in control (p = 0.019) and in infected group (p < 0.001), and viperin expression was significantly lower in infected group than in control (p = 0.001). The exposure to HBV significantly increased the expression of viperin in Swan 71 (p < 0.001). Exposure to HBV up-regulates viperin expression in vivo and in vitro in placental trophoblast, and lack of this up-regulation is associated with intrauterine transmission of HBV.